LOCUS BBa_K1152007 5846 bp DNA linear 25-Nov-2024 DEFINITION Helper construct for NRP-Indigoidine-tagging ACCESSION BBa_K1152007 VERSION BBa_K1152007.1 FEATURES Location/Qualifiers promoter 138..143 /label=-35 promoter 162..167 /label=-10 misc_feature 174..174 /label=start: misc_feature 228..901 /label=ccdB misc_feature 902..1822 /label=C_TycC2 misc_feature 1823..5677 /label=indC_plum misc_feature 5812..5831 /label=T7 TE polyA_site 5832..5845 /label=polya misc_feature 5838..5838 /label=stop: ORIGIN 1 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 61 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 121 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 181 agcggataac aatttcacac atactagaga aagaggagaa atactagact ggctgtgtat 241 aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt 301 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat 361 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg 421 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg 481 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata 541 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca 601 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg 661 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat 721 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt 781 catagcatac ctctttttga catacttcgg gtatacatat cagtatatat tcttataccg 841 caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc ggatccacgc 901 gtcggaaaaa accgagtaca ccgcgattca acccgtggca gcgcaggagt tttacccggt 961 ttcatctgcg caaaaaagaa tgtatatcct gcaacagttc gaaggcaacg gaatcagcta 1021 caacatttcg ggtgcgattc tcctggaagg aaagctggac tacgcccggt ttgccagcgc 1081 tgtgcaacag ctggcagagc gccacgaagc tttgcgcacc tcgttccacc ggatcgacgg 1141 cgagcctgtg caaaaagtgc acgaggaagt agaagtgccg cttttcatgc tggaggctcc 1201 cgaagaccag gcggagaaaa tcatgcgcga gtttgtccgt ccgtttgatc tcggggtcgc 1261 tccgctgatg cgaacaggtt tgctcaagct gggcaaagac cgccatttgt ttttgctcga 1321 catgcaccat atcatctcgg acggcgtttc ttcgcaaatt ttgctgcgtg aatttgccga 1381 gttgtaccag ggagcagact tgcagccgct ttcgctgcaa tacaaagatt tcgctgcttg 1441 gcaaaatgag ctgtttcaga cggaggcata caagaagcag gagcagcact ggctgaacac 1501 gtttgctgat gaaattccgc tcttgaacct gccgactgac tatccgcgcc ctagcgtgca 1561 aagctttgca ggcgatctcg tcctttttgc cgccggaaaa gaactgctgg agcggttgca 1621 acaggtagcg tcagaaacag gcaccacctt gtacatgatt ttgcttgccg cctacaatgt 1681 gctgctgtcc aagtataccg gccaggaaga catcatcgtc gggacgcctg tcgctggacg 1741 ttcccatgcg gacgtggaaa acatcatggg catattcgtg aacacattgg cgctgcgcaa 1801 ccagcctgcc agcagcaaaa cgatgttaga aaataatatt acacaatgtg actcaatcaa 1861 tgatgtttat cttaaagaag aagcaataac attgatggat atgcttgaga gtcaacttaa 1921 gcaccaggca gatggatatg ttgttattga tcaagaagaa tctctcagtt acgctgattt 1981 ctatttgagg gtgaaagaga tagggtattg tctgtcagaa attagctcaa agagttcggt 2041 gggtattggg cttttttgtg atccttctat agatttaatt tgtggtgcat ggggtatttt 2101 gtcagcggat aaagcttatt tgccgttatc gcctgactat ccaactgaac gcctcaaata 2161 tatgatagaa gattctggta ttgatgtgat ttttacgcaa tcgcacttaa aagcacagct 2221 acaggacatt gcaccaaaat cagtattaat tatgacacca gaagatgtcg ctctgacgat 2281 aaaaacacga acaatagaag atattctggg cacagttcaa gttcctaaac ccacgagtct 2341 ggcttatatt atttatacct ctggtagcac gggtaagcca aagggagtga tgattgaaca 2401 tcacagtatt gtaaatcaaa tgagatttct tgcaaaagcg ttcaaattag gatgtcattc 2461 ccggatttta cagaaaacac caatgagttt tgatgcggct caatgggaaa ttctagcgcc 2521 tgcaattggt ggtcaagtga ttatgggtcc tttaggttgc tatcgcgatc cggatgcaat 2581 tattaaaacc attcttcagc atcaagtaac gactttgcaa tgtgttccta ctttgctaca 2641 agcgttactg gataatccta attttttgga ttgcttatca ttgactcaag tattcagtgg 2701 gggagaagcg ctgacaacca aattagccac gcaatttttg aatagtttta ctcactgtga 2761 attaatcaat ttatatggcc cgacagaatg tacgattaat tcatcatttt tccgggtgac 2821 aaatgagact ttgccgaatt atcaaacctc tatttcgatt ggtgcacctg tagataatac 2881 cgaatactac gttcttgatg atgatagatt acctgtggcg gttggcgaaa ttggcgagct 2941 ttatatttcg ggtgctcaat tagcacgtgg ttatttgcat aaaccagaaa tgacaaaaga 3001 taaatttatt tgtaatcacc ttgtatcagg aactcaacat caatggttat atcgaacggg 3061 agatctggta accagagggg ctgatggtaa tacttatttt gttggtcggg ttgatagcca 3121 ggtcaaatta cgaggttacc gtattgagct tgatgaaata cgccatgcga ttgaagaaca 3181 tagctggata aagacggcgg caatgttaat taagaaggat gccagaacgg gtttccaaaa 3241 tctcatcgcg tgtgtggaat tagatgagaa agaagctgca ttgatggatc aaggtaatag 3301 tagctcacat cacaaatcaa aagccgataa actacaggtg aaagcccaac tttctaattc 3361 tggttgtcga agtgaagagt tatgtgaaaa tcgccctaca ttcttacttc cttatcaaga 3421 aggggagata aaacagagag aatatgcatt tggacgcaag acatatcgct attttgaggg 3481 aacagaaata acggtagaga aattaaaaaa attgctgaca gccactcaat cgaatgaaat 3541 tagctctttg ccactgagtc atctaaccct gaatgatttc ggttatgcat tgcgttattt 3601 tggtcagttt accagccatc aacgtttatt gcccaaatat gcctatgctt caccgggtgc 3661 tctctatgcg acacaaatgt attttgaatt gcataatgtt ctcggtttgg atgcggggat 3721 ttactattat catccagtga cacataagtt aataaaaatt tcaacattga gtcgtcggca 3781 aatgccaacg ataaaagtgc attttattgg caagcatgaa gccattgagc ccgtttataa 3841 gaacaatata caagaagttc tggaaatgga agcgggccat atgatgggtc tttttgatga 3901 cgtattaccg gaaattggct tgagtattgg taaaagtgaa tatcaagatg aatgtccaga 3961 ttggtatgat ggtgatattc aggattatta tcttggtgca tttgaaatat gtagctatga 4021 acatggattg ccgccatttg agactgatat ttatttacaa acacatgccc ataaaatacc 4081 tgagatgccg tgtggtttat atcacttttc taacggggaa tttgtacgaa taagtgatga 4141 tattgtccga aaaaaggatg ttattgcgat taatcagcaa gtttatgatc gctccagttt 4201 tggcgtgtca attattccac gctgtgtccc tgaatggcat tattatataa cactgggtcg 4261 tcggttacat gcgttacaaa gtaatccatt gtatattgga ttaatgtcat ctggttacag 4321 ttcgaagagc aataacgatt taccttcggc gaaaaggatg cgatctattc tcaatgcact 4381 tgatagacct atggcggcat tttatttctg cataggtggg ggtattagcc aagcgcaata 4441 tatgtgtgaa ggcatgaaag aagatgttgt tcatatgaaa gggccagttg aaatcattaa 4501 agatgatctt caacaacaac tccctcaata tatgattcca aataaggtat tagttttcga 4561 taaattacct ttgacggcca atggaaaagt ggattatcaa tctttatcag aatctaaagc 4621 cgtggagaat gtttcaacac agcgtctatt ggtgccatta catacagata ctgaaataag 4681 gcttggaaaa atttggatgg aagtactgaa atgggattca gtatctgccc tcgatgattt 4741 tttcgaaagt gggggtaatt ctttgatggc cgttgcaatg gttaataaga tcaatgcggc 4801 ctttaatatt cgttttccgt tacagatact ttttcaatct cctaatatag cagaattggc 4861 taagtggatt gaacagacag actctaaaac aatatcaaga ttaattttat tgaatcaggc 4921 aagcaaagac cccatttact gttggccggg tttgggcgga tatcctatga gtttgagatt 4981 gcttgctaat aaagtcgttc ctgatcgggc attttatgga atacaggcat atgggataaa 5041 cgagagtgaa ataccgtttt cttctatcca gagaatggca gaagaggata ttaaagagat 5101 aaagaaaata cagccagaag ggccatatat attgtgggga tattcatttg gtgcccgagt 5161 agcatttgaa gttgcatacc agcttgaaca agcgggagaa gaagttaacg cattgaattt 5221 attggctccg ggatctcctc atcttgatat gaagcaagcg gaatatatgg ataaaggcgc 5281 tgaatttact aatccggctt ttgttaaaat acttttttct gtattttctc gttcaatcaa 5341 cagcccaatg gttaaaactt gcttagaaca agtaaatagt gaaacgacat ttattaactt 5401 tatatgtagt cgttttaaaa acttggaacc atcattagta aaacgtatcg ttaggattgt 5461 gactttgact tatgatttca agtacagtat tgatgagctt tatcacagac acctaaaggc 5521 acctataact attttcaagg cgaatagaga taatgattca tttatcgagg aatcggatgt 5581 gatttcatca atgtcgccta aaataattga attaatatcg gatcactatc aactgttgga 5641 aagtgaaggt gttgctgaga ttgagaaaat aatctaataa taacgctgat agtgctagtg 5701 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 5761 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 5821 tcgggtgggc ctttctgcgt ttatat //