BBa_K1152009 1 BBa_K1152009 Sfp 4'-Phosphopanthetheinyl-transferase 2013-09-18T11:00:00Z 2015-05-08T01:09:29Z sfp originally comes from Bacillus subtilis strain 168. We amplfied it from E. coli BAP1 which carries the sfp gene. sfp encodes for a 4'-Phosphopanthetheinyl-transferase (PPTase) which transfers a 4'-PPT residue from CoA to a conserved serine residue in the T-Domain of NRPS modules, thus activating these enzymes. false false _1464_ 0 16405 9 In stock false We cloned sfp in a pSB3K3-derived plasmid with a lac promoter and the RBS BBa_B0029. false Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis annotation2369271 1 sfp cds range2369271 1 1 678 BBa_K1152009_sequence 1 atgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttcatgactttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagatgctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcagttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgatcttcccgacgctcatttcaacatttctcactccggccgctgggtcattggtgcgtttgattcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaagcgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagacagactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggcttatcgcttccgcttgattccttttcagtgcgcctgcatcaggacggacaagtatccattgagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctacaaaatggctgtatgcgccgcacaccctgatttccccgaggatatcacaatggtctcgtacgaagagcttttataatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z