BBa_K1154001 1 FUS1 pr Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone) 2013-09-16T11:00:00Z 2015-05-08T01:09:29Z The sequence is from the yeast genome (McCaffrey G, Clay FJ, Kelsay K, and Sprague GF Jr (1987) Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7 (8), 2680-2690). The FUS1 promoter is induced by Ste12, which is a key regulatory factor in the yeast mating pheromone response pathway. The promoter contains three copies of the Ste12 binding sequence. Genes driven by the promoter are induced when mating factor receptors on the cell's surface bind mating factor secreted by cells of the opposite mating type. false false _1466_ 0 12086 9 Not in stock false na false Adam Nighswander, Tanner Reeb and Bianca Maled annotation2352022 1 Ste12 binding range2352022 1 41 46 annotation2352020 1 Ste12 binding range2352020 1 14 19 annotation2352021 1 Ste 12 binding range2352021 1 25 30 BBa_K1154005 1 TEF2 pr Saccharomyces cerevisiae constitutive TEF2 promoter variant 2013-09-19T11:00:00Z 2015-05-08T01:09:30Z Yeast genome Constitutive TEF2 promoter variant. This variant has six additional nucleotides (tactag) at the 3' terminus of part BBa_K165037. false false _1466_ 0 12086 9 Not in stock false Edit false Adam Nighswander, Tanner Reeb and Bianca Maled BBa_K1154006 1 BBa_K1154006 Mating pheromone-induced IGPD and constitutive LDH expression in yeast 2013-09-19T11:00:00Z 2015-05-08T01:09:30Z xxx xxx false false _1466_ 0 12086 9 It's complicated false xxx false Adam Nighswander, Tanner Reeb and Bianca Maled component2357195 1 BBa_J63002 component2357192 1 BBa_K1154001 component2357193 1 BBa_K1154002 component2357197 1 BBa_K1154003 component2357199 1 BBa_J63002 component2357196 1 BBa_K1154005 annotation2357193 1 BBa_K1154002 range2357193 1 202 864 annotation2357192 1 BBa_K1154001 range2357192 1 1 201 annotation2357199 1 BBa_J63002 range2357199 1 2498 2722 annotation2357195 1 BBa_J63002 range2357195 1 865 1089 annotation2357197 1 BBa_K1154003 range2357197 1 1499 2497 annotation2357196 1 BBa_K1154005 range2357196 1 1090 1498 BBa_J63002 1 tADH1 ADH1 terminator from S. cerevisiae 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic DNA of S. cerevisiae ADH1 terminator from S. cerevisiae false true _97_ 0 545 97 It's complicated false starts with stop codon false Caroline Ajo-Franklin annotation1902851 1 ADH1 terminator range1902851 1 1 225 BBa_K1154003 1 LDHA Homo sapiens lactate dehydrogenase A (LDHA) 2013-09-16T11:00:00Z 2015-05-08T01:09:29Z Synthetic coding sequence is derived from the polypeptide sequence of Homo sapiens lactate dehydrogenase subunit A. Subunit A of L-lactate-specific dehydrogenase (M-isozyme) catalyzes the interconversion of pyruvate plus NADH and L-lactate plus NAD in the final step of anaerobic glycolysis. false false _1466_ 0 12086 9 Not in stock false The sequence is optimized for high codon bias in yeast and conformity with current BioBrick standards. false Adam Nighswander, Tanner Reeb and Bianca Maled BBa_K1154002 1 IGPD Saccharomyces cerevisiae imidazoleglycerol-phosphate dehydratase (IGPD) 2013-09-16T11:00:00Z 2015-05-08T01:09:29Z Coding sequence is from the HIS3/YOR202W locus on yeast chromosome XV from coordinates 721946 to 722608. The Saccharomyces cerevisiae HIS3 gene encodes imidazoleglycerol-phosphate dehydratase (IGPD), which catalyzes the interconversion of D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate and 3-(imidazol-4-yl)-2-oxopropyl phosphate plus H2O. The dehydration reaction is a key step in the biosynthesis of histidine in many bacteria, fungi and plants. The gene is routinely used as a selectable marker in yeast histidine auxotrophs harboring a genomic his3 mutation (e.g., his3-delta1). Although, it also compliments Escherichia coli hisB mutations (e.g., ΔhisB720::kan). false false _1466_ 0 12086 9 Not in stock false na false Adam Nighswander, Tanner Reeb and Bianca Maled BBa_K1154002_sequence 1 atgactgaacaaaaagctttggttaaaagaattactaatgaaactaaaattcaaattgctatttctttgaaaggtggtccattggctattgaacattctatttttccagaaaaagaagctgaagctgttgctgaacaagctactcaatctcaagttattaatgttcatactggtattggttttttggatcatatgattcatgctttggctaaacattctggttggtctttgattgttgaatgtattggtgatttgcatattgatgatcatcatactactgaagattgtggtattgctttgggtcaagcttttaaagaagctttgggtgctgttagaggtgttaaaagatttggttctggttttgctccattggatgaagctttgtcaagagctgttgttgatttgtctaatagaccatatgctgttgttgaattgggtttgcaaagagaaaaagttggtgatttgtcttgtgaaatgattccacattttttggaatcttttgctgaagcttcaagaattactttgcatgttgattgtttgagaggtaaaaatgatcatcatagatctgaatctgcttttaaagctttggctgttgctattagagaagctacttctccaaatggtactaatgatgttccatctactaaaggtgttttgatgtaa BBa_K1154001_sequence 1 tttggtgcgatgatgaaacaaacatgaaacgtctgtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatcttttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaa BBa_J63002_sequence 1 taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K1154006_sequence 1 tttggtgcgatgatgaaacaaacatgaaacgtctgtaatttgaaacaaataacgtaattctcgggattggttttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttggatacgacatcctttatcttttttcctttaagagcaggatataagccatcaagtttctgaaaatcaaaaatgactgaacaaaaagctttggttaaaagaattactaatgaaactaaaattcaaattgctatttctttgaaaggtggtccattggctattgaacattctatttttccagaaaaagaagctgaagctgttgctgaacaagctactcaatctcaagttattaatgttcatactggtattggttttttggatcatatgattcatgctttggctaaacattctggttggtctttgattgttgaatgtattggtgatttgcatattgatgatcatcatactactgaagattgtggtattgctttgggtcaagcttttaaagaagctttgggtgctgttagaggtgttaaaagatttggttctggttttgctccattggatgaagctttgtcaagagctgttgttgatttgtctaatagaccatatgctgttgttgaattgggtttgcaaagagaaaaagttggtgatttgtcttgtgaaatgattccacattttttggaatcttttgctgaagcttcaagaattactttgcatgttgattgtttgagaggtaaaaatgatcatcatagatctgaatctgcttttaaagctttggctgttgctattagagaagctacttctccaaatggtactaatgatgttccatctactaaaggtgttttgatgtaataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctcccattacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaactactagatggctactttgaaagatcaattgatttataatttgttgaaagaagaacaaactccacaaaataaaattactgttgttggtgttggtgctgttggtatggcttgtgctatttctattttgatgaaagatttggctgatgaattggctttggttgatgttattgaagataaattgaaaggtgaaatgatggatttgcaacatggttctttgtttttgagaactccaaaaattgtttctggtaaagattataatgttactgctaattctaaattggttattattactgctggtgctagacaacaagaaggtgaatcaagattgaatttggttcaaagaaatgttaatatttttaaatttattattccaaatgttgttaaatattctccaaattgtaaattgttgattgtttctaatccagttgatattttgacttatgttgcttggaaaatttctggttttccaaaaaatagagttattggttctggttgtaatttggattctgctagatttagatatttgatgggtgaaagattgggtgttcatccattgtcttgtcatggttgggttttgggtgaacatggtgattcttctgttccagtttggtctggtatgaatgttgctggtgtttctttgaaaactttgcatccagatttgggtactgataaagataaagaacaatggaaagaagttcataaacaagttgttgaatctgcttatgaagttattaaattgaaaggttatacttcttgggctattggtttgtctgttgctgatttggctgaatctattatgaaaaatttgagaagagttcatccagtttctactatgattaaaggtttgtatggtattaaagatgatgtttttttgtctgttccatgtattttgggtcaaaatggtatttctgatttggttaaagttactttgacttctgaagaagaagctagattgaaaaaatctgctgatactttgtggggtattcaaaaagaattgcaattttaataataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K1154005_sequence 1 attacccataaggttgtttgtgacggcgtcgtacaagagaacgtgggaactttttaggctcaccaaaaaagaaagaaaaaatacgagttgctgacagaagcctcaagaaaaaaaaaattcttcttcgactatgctggaggcagagatgatcgagccggtagttaactatatatagctaaattggttccatcaccttcttttctggtgtcgctccttctagtgctatttctggcttttcctatttttttttttccatttttctttctctctttctaatatataaattctcttgcattttctatttttctctctatctattctacttgtttattcccttcaaggtttttttttaaggagtacttgtttttagaatatacggtcaacgaactataattaactaaactactag BBa_K1154003_sequence 1 atggctactttgaaagatcaattgatttataatttgttgaaagaagaacaaactccacaaaataaaattactgttgttggtgttggtgctgttggtatggcttgtgctatttctattttgatgaaagatttggctgatgaattggctttggttgatgttattgaagataaattgaaaggtgaaatgatggatttgcaacatggttctttgtttttgagaactccaaaaattgtttctggtaaagattataatgttactgctaattctaaattggttattattactgctggtgctagacaacaagaaggtgaatcaagattgaatttggttcaaagaaatgttaatatttttaaatttattattccaaatgttgttaaatattctccaaattgtaaattgttgattgtttctaatccagttgatattttgacttatgttgcttggaaaatttctggttttccaaaaaatagagttattggttctggttgtaatttggattctgctagatttagatatttgatgggtgaaagattgggtgttcatccattgtcttgtcatggttgggttttgggtgaacatggtgattcttctgttccagtttggtctggtatgaatgttgctggtgtttctttgaaaactttgcatccagatttgggtactgataaagataaagaacaatggaaagaagttcataaacaagttgttgaatctgcttatgaagttattaaattgaaaggttatacttcttgggctattggtttgtctgttgctgatttggctgaatctattatgaaaaatttgagaagagttcatccagtttctactatgattaaaggtttgtatggtattaaagatgatgtttttttgtctgttccatgtattttgggtcaaaatggtatttctgatttggttaaagttactttgacttctgaagaagaagctagattgaaaaaatctgctgatactttgtggggtattcaaaaagaattgcaattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z