BBa_K1156000
1
BBa_K1156000
RBS_T7_Pet(mCherry)
2013-09-16T11:00:00Z
2015-05-08T01:09:30Z
A sequence optimized for E. coli for a PET beta barrel auto-transporter expressing mCherry was obtained from Sevastynovich et al."A generalised module for the selective extracellular accumulation of recombinant proteins." Microbial Cell Factories. 2012. The sequence was assembled from synthesized 500bp blocks through IDTdna "Gblock" synthesis.
This is a composite part of an autodisplay technology translocating a fluorescent protein (mCherry). This part includes an T7 promoter with a lacI operator as well as an RBS(AGGA). The fluorescent protein will cleave post-translationally. This part can be used to quantify the surface density and activity of autodisplay technology. PET is a beta-barrel autodisplay technology native to E.coli. mCherry is a derivative of RFP.
false
false
_1468_
0
18496
9
It's complicated
false
To comply with BioBrick standard 10, Phe-76 was re-optimized to remove a EcoRI site within the part. A T7 promoter with a lacI operator was added with the RBS (AGGA) to the PET_mCherry. A standard assembly 10 prefix and suffix was then added.
false
Haylee Bachman
annotation2353227
1
Autochaperone
range2353227
1
1235
1546
annotation2353261
1
RBS
range2353261
1
9
12
annotation2353210
1
Signal Peptide
range2353210
1
119
274
annotation2353223
1
mCherry
range2353223
1
317
1024
annotation2353238
1
Start
range2353238
1
119
121
annotation2353239
1
Stop
range2353239
1
2459
2466
annotation2353230
1
Translocator Domain
range2353230
1
1547
2458
BBa_K1156000_sequence
1
gaagcgagaggatcttaaggctagagtaccaatacgactcactatagggagaggaattgtgagcggataacaattcccactagaaataattttgtttaacttaagaaggaaatactagatgaacaaaatctactctatcaaatactctgcggcgaccggcggtctgatcgcggtttctgagctcgccaaaaaagttatctgcaaaaccaaccgtaaaatctctgcggcgctgctgtctctggcggttatctcttacaccaacatcatctacgcggcgaacatggatatctctaaagcgtgggcgcgtgactacctaatggtttctaaaggtgaagaagacaacatggctatcatcaaagaatttatgcgtttcaaagttcacatggaaggttctgtgaacggtcacgaatttgaaatcgaaggtgaaggtgaaggtcgtccgtatgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtctccgcagttcatgtacggttctaaagcgtatgttaaacacccggctgacatcccggactacctgaaactgtctttcccggaaggtttcaaatgggaacgtgttatgaactttgaagacggtggtgttgttaccgttacccaggactcttctctgcaagacggtgaatttatctacaaagttaaactgcgtggcaccaacttcccgtctgacggtccggttatgcagaaaaaaacgatgggttgggaagcgtcttctgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaacagcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacaaagctaaaaagccggttcaactgccgggtgcttacaacgtgaacatcaaactggacatcacctctcacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactctaccggcggtatggacgaactgtataaagacaaagacaactctgcgacccgttactctcagttcgcgctggcgatgctggacggtttcgacacctcttaccagggttctatcaaagcggcgcagtcttctctggcgatgaacaacgcgctgtggaaagttaccggcaactctgaactgaaaaaacttaactctaccggctctatggttctgttcaacggtggtaaaaacatcttcaacaccctgaccgttgacgaactgaccacctctaactctgcgttcgttatgcgtaccaacacccagcaggcggaccaactgatcgttaaaaacaaactggaaggtgcgaacaacctgctgctggtcgacttcatcgaaaaaaaaggtaacgacaaaaacggtctgaacatcgacctggttaaagcgccggaaaacacctctaaagacgttttcaaaaccgaaacccagaccatcggtttctctgacgttaccccggaaatcaaacagcaggaaaaagacggtaaatctgtttggaccctgaccggctacaaaaccgttgcgaacgcggacgcggcgaaaaaagcgacctctctgatgtctggtggttacaaagcgttcctggcggaagttaacaacctgaacaaacgtatgggtgacctgcgtgacatcaacggtgaagcgggtgcgtgggcgcgtatcatgtctggtaccggttcggccggtggtggtttctctgacaactacacccacgttcaggttggtgcggacaacaaacacgaactggacggtctggacctgttcaccggtgttaccatgacctacaccgactctcacgccggctctgacgctttctctggtgaaaccaaatctgttggtgcgggtctgtacgcttctgcgatgtttgaatctggtgcgtacatcgacctgatcggtaaatacgttcaccacgacaacgaatacactgcaaccttcgcgggtctgggtacccgtgactactcttctcactcttggtacgcgggtgcggaagttggttaccgttaccacgttaccgactctgcgtggatcgaaccgcaggcggaactggtttacggtgcggtttctggtaaacagttctcttggaaagaccagggtatgaacctgaccatgaaagacaaagacttcaacccgctgatcggtcgtaccggcgttgacgtcggtaaatctttctctggtaaagactggaaagttaccgcgcgtgcgggtctgggttaccagttcgacctgttcgctaacggtgaaaccgttctgcgtgacgcttctggtgaaaaacgtatcaaaggtgaaaaagacggtcgtatgctgatgaacgtgggtctgaacgcggaaatccgtgacaacgtgcgtttcggtctggaatttgagaaatctgcgttcggtaaatacaacgtggacaacgcgatcaacgcgaacttccgttactctttctgataataatac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z