BBa_K1156000 1 BBa_K1156000 RBS_T7_Pet(mCherry) 2013-09-16T11:00:00Z 2015-05-08T01:09:30Z A sequence optimized for E. coli for a PET beta barrel auto-transporter expressing mCherry was obtained from Sevastynovich et al."A generalised module for the selective extracellular accumulation of recombinant proteins." Microbial Cell Factories. 2012. The sequence was assembled from synthesized 500bp blocks through IDTdna "Gblock" synthesis. This is a composite part of an autodisplay technology translocating a fluorescent protein (mCherry). This part includes an T7 promoter with a lacI operator as well as an RBS(AGGA). The fluorescent protein will cleave post-translationally. This part can be used to quantify the surface density and activity of autodisplay technology. PET is a beta-barrel autodisplay technology native to E.coli. mCherry is a derivative of RFP. false false _1468_ 0 18496 9 It's complicated false To comply with BioBrick standard 10, Phe-76 was re-optimized to remove a EcoRI site within the part. A T7 promoter with a lacI operator was added with the RBS (AGGA) to the PET_mCherry. A standard assembly 10 prefix and suffix was then added. false Haylee Bachman annotation2353227 1 Autochaperone range2353227 1 1235 1546 annotation2353261 1 RBS range2353261 1 9 12 annotation2353210 1 Signal Peptide range2353210 1 119 274 annotation2353223 1 mCherry range2353223 1 317 1024 annotation2353238 1 Start range2353238 1 119 121 annotation2353239 1 Stop range2353239 1 2459 2466 annotation2353230 1 Translocator Domain range2353230 1 1547 2458 BBa_K1156000_sequence 1 gaagcgagaggatcttaaggctagagtaccaatacgactcactatagggagaggaattgtgagcggataacaattcccactagaaataattttgtttaacttaagaaggaaatactagatgaacaaaatctactctatcaaatactctgcggcgaccggcggtctgatcgcggtttctgagctcgccaaaaaagttatctgcaaaaccaaccgtaaaatctctgcggcgctgctgtctctggcggttatctcttacaccaacatcatctacgcggcgaacatggatatctctaaagcgtgggcgcgtgactacctaatggtttctaaaggtgaagaagacaacatggctatcatcaaagaatttatgcgtttcaaagttcacatggaaggttctgtgaacggtcacgaatttgaaatcgaaggtgaaggtgaaggtcgtccgtatgaaggcacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtctccgcagttcatgtacggttctaaagcgtatgttaaacacccggctgacatcccggactacctgaaactgtctttcccggaaggtttcaaatgggaacgtgttatgaactttgaagacggtggtgttgttaccgttacccaggactcttctctgcaagacggtgaatttatctacaaagttaaactgcgtggcaccaacttcccgtctgacggtccggttatgcagaaaaaaacgatgggttgggaagcgtcttctgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaacagcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacaaagctaaaaagccggttcaactgccgggtgcttacaacgtgaacatcaaactggacatcacctctcacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactctaccggcggtatggacgaactgtataaagacaaagacaactctgcgacccgttactctcagttcgcgctggcgatgctggacggtttcgacacctcttaccagggttctatcaaagcggcgcagtcttctctggcgatgaacaacgcgctgtggaaagttaccggcaactctgaactgaaaaaacttaactctaccggctctatggttctgttcaacggtggtaaaaacatcttcaacaccctgaccgttgacgaactgaccacctctaactctgcgttcgttatgcgtaccaacacccagcaggcggaccaactgatcgttaaaaacaaactggaaggtgcgaacaacctgctgctggtcgacttcatcgaaaaaaaaggtaacgacaaaaacggtctgaacatcgacctggttaaagcgccggaaaacacctctaaagacgttttcaaaaccgaaacccagaccatcggtttctctgacgttaccccggaaatcaaacagcaggaaaaagacggtaaatctgtttggaccctgaccggctacaaaaccgttgcgaacgcggacgcggcgaaaaaagcgacctctctgatgtctggtggttacaaagcgttcctggcggaagttaacaacctgaacaaacgtatgggtgacctgcgtgacatcaacggtgaagcgggtgcgtgggcgcgtatcatgtctggtaccggttcggccggtggtggtttctctgacaactacacccacgttcaggttggtgcggacaacaaacacgaactggacggtctggacctgttcaccggtgttaccatgacctacaccgactctcacgccggctctgacgctttctctggtgaaaccaaatctgttggtgcgggtctgtacgcttctgcgatgtttgaatctggtgcgtacatcgacctgatcggtaaatacgttcaccacgacaacgaatacactgcaaccttcgcgggtctgggtacccgtgactactcttctcactcttggtacgcgggtgcggaagttggttaccgttaccacgttaccgactctgcgtggatcgaaccgcaggcggaactggtttacggtgcggtttctggtaaacagttctcttggaaagaccagggtatgaacctgaccatgaaagacaaagacttcaacccgctgatcggtcgtaccggcgttgacgtcggtaaatctttctctggtaaagactggaaagttaccgcgcgtgcgggtctgggttaccagttcgacctgttcgctaacggtgaaaccgttctgcgtgacgcttctggtgaaaaacgtatcaaaggtgaaaaagacggtcgtatgctgatgaacgtgggtctgaacgcggaaatccgtgacaacgtgcgtttcggtctggaatttgagaaatctgcgttcggtaaatacaacgtggacaacgcgatcaacgcgaacttccgttactctttctgataataatac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z