LOCUS       BBa_K1157006            1871 bp    DNA     linear       25-Nov-2024
DEFINITION  pRhl-RBS-mCherry-tt-Pc-RBS-RhlR-tt
ACCESSION   BBa_K1157006
VERSION     BBa_K1157006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..793
                     /label=mCherry
     misc_feature    434..434
                     /label=C->T (removing PstI site)
     misc_feature    83..796
                     /label=BBa_J06504
     stem_loop       816..859
                     /label=stem_loop
     misc_feature    805..884
                     /label=BBa_B0010
     terminator      805..884
                     /label=BBa_B0010
     stem_loop       900..919
                     /label=T7 TE
     misc_feature    926..926
                     /label=stop
     polyA_site      920..933
                     /label=polya
     misc_feature    893..933
                     /label=BBa_B0012
     terminator      893..933
                     /label=BBa_B0012
     terminator      805..933
                     /label=BBa_B0015
     promoter        942..976
                     /label=BBa_J23100
     RBS             985..999
                     /label=RBS-1\Strong
     RBS             992..996
                     /label=RBS
     misc_feature    985..999
                     /label=BBa_B0030
     RBS             985..999
                     /label=BBa_B0030
     CDS             1006..1734
                     /label=BBa_C0171
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     terminator      1743..1871
                     /label=BBa_B0015
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatggtgag caagggcgag gaggataaca tggccatcat
      121 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      181 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      241 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      301 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      361 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      421 ccaggactcc tccttgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      481 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      541 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      601 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      661 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      721 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      781 gctgtacaag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      901 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacggct agctcagtcc
      961 taggtacagt gctagctact agagattaaa gaggagaaat actagatgag gaatgacgga
     1021 ggctttttgc tgtggtggga cggtttgcgt agcgagatgc agccgatcca cgacagccag
     1081 ggcgtgttcg ccgtcctgga aaaggaagtg cggcgcctgg gcttcgatta ctacgcctat
     1141 ggcgtgcgcc acacgattcc cttcacccgg ccgaagaccg aggtccatgg cacctatccc
     1201 aaggcctggc tggagcgata ccagatgcag aactacgggg ccgtggatcc ggcgatcctc
     1261 aacggcctgc gctcctcgga aatggtggtc tggagcgaca gcctgttcga ccagagccgg
     1321 atgctctgga acgaggctcg cgattggggc ctctgtgtcg gcgcgacctt gccgatccgc
     1381 gcgccgaaca atttgctcag cgtgctttcc gtggcgcgcg accagcagaa catctccagc
     1441 ttcgagcgcg aggaaatccg cctgcggctg cgttgcatga tcgagttgct gacccagaag
     1501 ctgaccgacc tggagcatcc gatgctgatg tccaacccgg tctgcctgag ccatcgcgaa
     1561 cgcgagatcc tgcaatggac cgccgacggc aagagttccg gggaaatcgc catcatcctg
     1621 agcatctccg agagcacggt gaacttccac cacaagaaca tccagaagaa gttcgacgcg
     1681 ccgaacaaga cgctggctgc cgcctacgcc gcggcgctgg gtctcatcta ataatactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat a
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