##gff-version 3
##sequence-region BBa_K1157006 1 1871
BBa_K1157006	.	promoter	942	976	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157006	.	terminator	1743	1871	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157006	.	terminator	1831	1871	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157006	.	engineered_region	1831	1871	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157006	.	stem_loop	1838	1857	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157006	.	polyA_site	1858	1871	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157006	.	stop_codon	1864	1864	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157006	.	terminator	1743	1822	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157006	.	engineered_region	1743	1822	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157006	.	stem_loop	1754	1797	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157006	.	CDS	1006	1734	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157006	.	ribosome_entry_site	985	999	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157006	.	ribosome_entry_site	992	996	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157006	.	engineered_region	985	999	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157006	.	ribosome_entry_site	985	999	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157006	.	terminator	805	933	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157006	.	terminator	893	933	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157006	.	engineered_region	893	933	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157006	.	stem_loop	900	919	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157006	.	polyA_site	920	933	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157006	.	stop_codon	926	926	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157006	.	terminator	805	884	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157006	.	engineered_region	805	884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157006	.	stem_loop	816	859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157006	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157006	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157006	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157006	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157006	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157006	.	engineered_region	83	796	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1157006	.	CDS	83	793	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1157006	.	sequence_alteration	434	434	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1157006	.	ribosome_entry_site	62	76	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157006	.	ribosome_entry_site	69	73	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157006	.	engineered_region	62	76	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157006	.	ribosome_entry_site	62	76	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K1157006
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatac
tagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggct
ccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaa
ggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtg
aagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcg
aggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcgg
caccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtacccc
gaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaaga
ccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaa
cgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaag
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacgg
ctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggaatgacggaggctttt
tgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaagga
agtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtc
catggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacg
gcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcga
ttggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgac
cagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagc
tgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggac
cgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaac
atccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata