LOCUS       BBa_K1157008            2018 bp    DNA     linear       15-Nov-2024
DEFINITION  Las specific quorum sensing sensor
ACCESSION   BBa_K1157008
VERSION     BBa_K1157008.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..885
                     /label=mTagBFP
     CDS             187..891
                     /label=BBa_K592100
     stem_loop       911..954
                     /label=stem_loop
     misc_feature    900..979
                     /label=BBa_B0010
     terminator      900..979
                     /label=BBa_B0010
     stem_loop       995..1014
                     /label=T7 TE
     misc_feature    1021..1021
                     /label=stop
     polyA_site      1015..1028
                     /label=polya
     misc_feature    988..1028
                     /label=BBa_B0012
     terminator      988..1028
                     /label=BBa_B0012
     terminator      900..1028
                     /label=BBa_B0015
     promoter        1037..1071
                     /label=BBa_J23100
     RBS             1080..1094
                     /label=RBS-1\Strong
     RBS             1087..1091
                     /label=RBS
     misc_feature    1080..1094
                     /label=BBa_B0030
     RBS             1080..1094
                     /label=BBa_B0030
     CDS             1101..1817
                     /label=lasR
     misc_feature    1818..1850
                     /label=LVA
     misc_feature    1580..1580
                     /label=G
     misc_feature    1857..1881
                     /label=Help:Barcodes
     CDS             1101..1881
                     /label=BBa_C0079
     stem_loop       1901..1944
                     /label=stem_loop
     misc_feature    1890..1969
                     /label=BBa_B0010
     terminator      1890..1969
                     /label=BBa_B0010
     stem_loop       1985..2004
                     /label=T7 TE
     misc_feature    2011..2011
                     /label=stop
     polyA_site      2005..2018
                     /label=polya
     misc_feature    1978..2018
                     /label=BBa_B0012
     terminator      1978..2018
                     /label=BBa_B0012
     terminator      1890..2018
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatga gcgaactgat caaagagaac atgcacatga agctgtacat ggaaggcacc
      241 gttgacaacc accactttaa gtgcacgtct gagggtgagg gtaagccgta cgaaggcacc
      301 caaaccatgc gtatcaaagt tgtggagggc ggtccactgc cgttcgcttt tgacattctg
      361 gcgaccagct tcctgtacgg ttccaaaacg ttcattaacc atactcaggg cattccggat
      421 ttcttcaaac agagctttcc ggaaggtttc acctgggagc gtgtcaccac gtatgaagat
      481 ggtggtgtgt tgaccgccac ccaagatacc tccctgcaag atggctgtct gatctataac
      541 gtgaaaattc gtggcgtcaa ctttacgagc aatggtccgg tgatgcagaa gaaaaccctg
      601 ggttgggagg cgtttacgga aaccctgtat ccggccgatg gtggcctgga gggccgtaac
      661 gacatggcac tgaagctggt tggtggcagc catttgatcg caaatatcaa gacgacgtac
      721 cgcagcaaga aaccggcgaa aaatctgaag atgccgggtg tttactatgt cgactaccgt
      781 ctggaacgca ttaaagaagc gaataatgag acttacgtgg agcagcacga ggttgcagtc
      841 gcgcgctatt gcgacttgcc tagcaagctg ggtcataaac tgaattaata atactagagc
      901 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      961 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1021 cgtttatata ctagagttga cggctagctc agtcctaggt acagtgctag ctactagaga
     1081 ttaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
     1141 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
     1201 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
     1261 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
     1321 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
     1381 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
     1441 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
     1501 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
     1561 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
     1621 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
     1681 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
     1741 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
     1801 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact
     1861 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1921 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1981 cactggctca ccttcgggtg ggcctttctg cgtttata
//