##gff-version 3
##sequence-region BBa_K1157008 1 2018
BBa_K1157008	.	CDS	187	891	.	+	0	ID=BBa_K592100;Name=BBa_K592100
BBa_K1157008	.	CDS	187	885	.	+	0	ID=annotation2135490;Name=mTagBFP;Parent=BBa_K592100
BBa_K1157008	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K1157008	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1157008	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1157008	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1157008	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1157008	.	terminator	900	1028	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157008	.	terminator	900	979	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157008	.	engineered_region	900	979	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157008	.	stem_loop	911	954	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157008	.	terminator	988	1028	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157008	.	stop_codon	1021	1021	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157008	.	polyA_site	1015	1028	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157008	.	stem_loop	995	1014	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157008	.	engineered_region	988	1028	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157008	.	promoter	1037	1071	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157008	.	ribosome_entry_site	166	180	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157008	.	ribosome_entry_site	173	177	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157008	.	engineered_region	166	180	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157008	.	ribosome_entry_site	166	180	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157008	.	terminator	1890	2018	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157008	.	terminator	1890	1969	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157008	.	engineered_region	1890	1969	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157008	.	stem_loop	1901	1944	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157008	.	terminator	1978	2018	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157008	.	stop_codon	2011	2011	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157008	.	polyA_site	2005	2018	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157008	.	stem_loop	1985	2004	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157008	.	engineered_region	1978	2018	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157008	.	ribosome_entry_site	1080	1094	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157008	.	ribosome_entry_site	1087	1091	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157008	.	engineered_region	1080	1094	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157008	.	ribosome_entry_site	1080	1094	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157008	.	CDS	1101	1881	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K1157008	.	tag	1818	1850	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K1157008	.	CDS	1101	1817	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K1157008	.	sequence_alteration	1580	1580	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K1157008	.	engineered_tag	1857	1881	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
>BBa_K1157008
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagattaaagaggagaaatactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggc
accgttgacaaccaccactttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatca
aagttgtggagggcggtccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcat
taaccatactcagggcattccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtat
gaagatggtggtgtgttgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtg
gcgtcaactttacgagcaatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatcc
ggccgatggtggcctggagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaag
acgacgtaccgcagcaagaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgca
ttaaagaagcgaataatgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagct
gggtcataaactgaattaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggcc
ttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcg
accttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaa
ctacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccag
agcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccg
ccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagc
ggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaa
agcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagt
ggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatggg
aaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata