BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_K1157009
1
BBa_K1157009
Quorum sensing receptor pqsR generator
2013-09-09T11:00:00Z
2015-05-08T01:09:30Z
B0030-K1157003-B0015
AbaR is a 12C quorum sensing molecule receptor. This part contains it with RBS and double terminator.
false
false
_1469_
0
16549
9
It's complicated
false
no special consideration
false
Wei-yu Chi
component2337980
1
BBa_B0030
component2337982
1
BBa_K1157001
component2337989
1
BBa_B0015
annotation2337989
1
BBa_B0015
range2337989
1
1029
1157
annotation2337980
1
BBa_B0030
range2337980
1
1
15
annotation2337982
1
BBa_K1157001
range2337982
1
22
1020
BBa_K1157001
1
PQSR
PQSR
2013-08-27T11:00:00Z
2015-05-08T01:09:30Z
It comes from Pseudomonas ATCC 27853 genome, functioning as one of the quorum sensing systems in the bacterium.
PQSR is a quorum sensing receptor which is similar to the LuxI-LuxR system. It is found in Pseudomonas and uses 2-heptyl-3-hydroxy-4-quinolone as quorum sensing molecule. We use it as one of our quorum sensing receptors and hope to function as a biosensor.
false
false
_1469_
0
16435
9
It's complicated
false
There are no PstI, EcoRI, XbaI or SpeI restriction sites in the sequence.
false
Lin Ang Chieh
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1157001_sequence
1
atgcctattcataacctgaatcacgtgaacatgttcctccaggtcatcgcctccggttcgatttcctccgctgcgcggatcctgcgcaagtcgcacaccgcggtcagctcggcggtcagcaacctggaaatcgacctgtgcgtggagctggtccgtcgggacggctacaaggtcgaacccaccgagcaggcgcttcgcctgatcccttacatgcgcagcctgctgaactaccagcagctgatcggcgacatcgccttcaatctcaacaagggtccgcgcaatctccgggtgctgctggacaccgccatcccgccgtcgttctgcgatacggtgagcagcgtactgctcgacgatttcaacatggtcagcctgatacgcacctcgcccgccgatagcctggcgacgatcaagcaggacaacgcggaaatcgatatcgccatcaccatcgacgaggaactgaagatctcccgcttcaaccagtgcgtgctcggctacaccaaggcgttcgtcgtcgcccatccgcagcacccgttgtgcaatgcctccctgcacagcatcgcgagcctggccaattaccggcagatcagcctcggcagccgctccgggcagcattcgaacctgctgcggccggtcagcgacaaggtgctcttcgtggaaaacttcgacgacatgctgcgtctggtggaagccggcgtcggatggggcatcgcgccgcattatttcgtcgaggaacgcctgcgcaacggtaccctggcagtcctcagcgaactctacgaaccgggcggcatcgacaccaaggtgtattgctactacaacaccgcgctggaatccgagcgcagcttcctgcgctttctcgaaagcgcccgccagcgcctgcgcgaactgggccgccagcgtttcgacgatgcgccggcctggcaaccgagcatcgtcgaaacggtgcagcgccgctcaggcccgaaggcgctcgcgtaccgccagcgcgccgcaccagagtag
BBa_K1157009_sequence
1
attaaagaggagaaatactagatgcctattcataacctgaatcacgtgaacatgttcctccaggtcatcgcctccggttcgatttcctccgctgcgcggatcctgcgcaagtcgcacaccgcggtcagctcggcggtcagcaacctggaaatcgacctgtgcgtggagctggtccgtcgggacggctacaaggtcgaacccaccgagcaggcgcttcgcctgatcccttacatgcgcagcctgctgaactaccagcagctgatcggcgacatcgccttcaatctcaacaagggtccgcgcaatctccgggtgctgctggacaccgccatcccgccgtcgttctgcgatacggtgagcagcgtactgctcgacgatttcaacatggtcagcctgatacgcacctcgcccgccgatagcctggcgacgatcaagcaggacaacgcggaaatcgatatcgccatcaccatcgacgaggaactgaagatctcccgcttcaaccagtgcgtgctcggctacaccaaggcgttcgtcgtcgcccatccgcagcacccgttgtgcaatgcctccctgcacagcatcgcgagcctggccaattaccggcagatcagcctcggcagccgctccgggcagcattcgaacctgctgcggccggtcagcgacaaggtgctcttcgtggaaaacttcgacgacatgctgcgtctggtggaagccggcgtcggatggggcatcgcgccgcattatttcgtcgaggaacgcctgcgcaacggtaccctggcagtcctcagcgaactctacgaaccgggcggcatcgacaccaaggtgtattgctactacaacaccgcgctggaatccgagcgcagcttcctgcgctttctcgaaagcgcccgccagcgcctgcgcgaactgggccgccagcgtttcgacgatgcgccggcctggcaaccgagcatcgtcgaaacggtgcagcgccgctcaggcccgaaggcgctcgcgtaccgccagcgcgccgcaccagagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z