LOCUS       BBa_K1157010            1871 bp    DNA     linear       10-Dec-2025
DEFINITION  Pc-RhlR-mCherry
ACCESSION   BBa_K1157010
VERSION     BBa_K1157010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..793
                     /label=BBa_C0171
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     terminator      802..930
                     /label=BBa_B0015
     misc_feature    939..958
                     /label=RhlR
     promoter        957..962
                     /label=-35
     promoter        980..985
                     /label=-10
     misc_feature    991..991
                     /label=start
     promoter        939..991
                     /label=BBa_R0071
     RBS             1000..1014
                     /label=RBS-1\Strong
     RBS             1007..1011
                     /label=RBS
     misc_feature    1000..1014
                     /label=BBa_B0030
     RBS             1000..1014
                     /label=BBa_B0030
     CDS             1021..1731
                     /label=mCherry
     misc_feature    1372..1372
                     /label=C->T (removing PstI site)
     misc_feature    1021..1734
                     /label=BBa_J06504
     stem_loop       1754..1797
                     /label=stem_loop
     misc_feature    1743..1822
                     /label=BBa_B0010
     terminator      1743..1822
                     /label=BBa_B0010
     stem_loop       1838..1857
                     /label=T7 TE
     misc_feature    1864..1864
                     /label=stop
     polyA_site      1858..1871
                     /label=polya
     misc_feature    1831..1871
                     /label=BBa_B0012
     terminator      1831..1871
                     /label=BBa_B0012
     terminator      1743..1871
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca
      121 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg
      181 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga
      241 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc
      301 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag
      361 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg
      421 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga
      481 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat
      541 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt
      601 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg
      661 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat
      721 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg
      781 tctcatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac
      961 cgttagcttt cgaattggct aaaaagtgtt ctactagaga ttaaagagga gaaatactag
     1021 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     1081 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     1141 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     1201 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     1261 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     1321 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cttgcaggac
     1381 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     1441 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     1501 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     1561 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     1621 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     1681 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta ataatactag
     1741 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1801 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1861 ctgcgtttat a
//