##gff-version 3
##sequence-region BBa_K1157010 1 1871
BBa_K1157010	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157010	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157010	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157010	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157010	.	promoter	939	991	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157010	.	promoter	957	962	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157010	.	promoter	980	985	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157010	.	non_covalent_binding_site	939	958	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157010	.	start_codon	991	991	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157010	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157010	.	CDS	65	793	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157010	.	terminator	1743	1871	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157010	.	terminator	1743	1822	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157010	.	stem_loop	1754	1797	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157010	.	engineered_region	1743	1822	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157010	.	terminator	1831	1871	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157010	.	stem_loop	1838	1857	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157010	.	stop_codon	1864	1864	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157010	.	polyA_site	1858	1871	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157010	.	engineered_region	1831	1871	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157010	.	ribosome_entry_site	1000	1014	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157010	.	ribosome_entry_site	1000	1014	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157010	.	ribosome_entry_site	1007	1011	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157010	.	engineered_region	1000	1014	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157010	.	engineered_region	1021	1734	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1157010	.	sequence_alteration	1372	1372	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1157010	.	CDS	1021	1731	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1157010	.	terminator	802	930	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157010	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157010	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157010	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157010	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157010	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157010	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157010	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157010	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1157010
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggaatgacgga
ggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgg
aaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagac
cgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatc
ctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgagg
ctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggc
gcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacc
cagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgc
aatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccacca
caagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaa
tctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatggtga
gcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacgg
ccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaag
ggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccg
ccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcgg
cgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttc
ccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcg
ccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaa
ggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactac
accatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata