##gff-version 3
##sequence-region BBa_K1157012 1 2851
BBa_K1157012	.	engineered_region	1779	2714	.	+	0	ID=BBa_J52008;Name=BBa_J52008
BBa_K1157012	.	engineered_region	1779	2714	.	+	0	ID=annotation1902833;Name=Rluc;Parent=BBa_J52008
BBa_K1157012	.	CDS	1779	2714	.	+	0	ID=annotation1902845;Name=Rluc;Parent=BBa_J52008
BBa_K1157012	.	CDS	1021	1749	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157012	.	promoter	939	991	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157012	.	promoter	980	985	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157012	.	start_codon	991	991	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157012	.	promoter	957	962	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157012	.	non_covalent_binding_site	939	958	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157012	.	ribosome_entry_site	1000	1014	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	1007	1011	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157012	.	engineered_region	1000	1014	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	1000	1014	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157012	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157012	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157012	.	CDS	65	793	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157012	.	terminator	2723	2851	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157012	.	terminator	2723	2802	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157012	.	stem_loop	2734	2777	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157012	.	engineered_region	2723	2802	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157012	.	terminator	2811	2851	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157012	.	stem_loop	2818	2837	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157012	.	engineered_region	2811	2851	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157012	.	stop_codon	2844	2844	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157012	.	polyA_site	2838	2851	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157012	.	ribosome_entry_site	1758	1772	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	1765	1769	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157012	.	engineered_region	1758	1772	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157012	.	ribosome_entry_site	1758	1772	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157012	.	terminator	802	930	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157012	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157012	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157012	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157012	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157012	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157012	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157012	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157012	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1157012
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggaatgacgga
ggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgg
aaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagac
cgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatc
ctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgagg
ctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggc
gcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacc
cagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgc
aatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccacca
caagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaa
tctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatgagga
atgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgc
cgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccgg
ccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatc
cggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctg
gaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctt
tccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagt
tgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcga
gatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaac
ttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtc
tcatctaataatactagagattaaagaggagaaatactagatggcttccaaggtgtacgaccccgagcaacgcaaacgc
atgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccg
agaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctca
catcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatat
cgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggcc
acgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagag
tgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggc
gagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgagg
agttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccc
tctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctg
cctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccg
agttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgt
ggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata