LOCUS BBa_K1157013 3627 bp DNA linear 22-Nov-2024 DEFINITION pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI ACCESSION BBa_K1157013 VERSION BBa_K1157013.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..72 /label=RBS-1\Strong RBS 65..69 /label=RBS misc_feature 58..72 /label=BBa_B0030 RBS 58..72 /label=BBa_B0030 CDS 79..789 /label=mCherry misc_feature 430..430 /label=C->T (removing PstI site) misc_feature 79..792 /label=BBa_J06504 stem_loop 812..855 /label=stem_loop misc_feature 801..880 /label=BBa_B0010 terminator 801..880 /label=BBa_B0010 stem_loop 896..915 /label=T7 TE misc_feature 922..922 /label=stop polyA_site 916..929 /label=polya misc_feature 889..929 /label=BBa_B0012 terminator 889..929 /label=BBa_B0012 terminator 801..929 /label=BBa_B0015 promoter 938..972 /label=BBa_J23100 RBS 981..995 /label=RBS-1\Strong RBS 988..992 /label=RBS misc_feature 981..995 /label=BBa_B0030 RBS 981..995 /label=BBa_B0030 CDS 1002..1730 /label=BBa_C0171 stem_loop 1750..1793 /label=stem_loop misc_feature 1739..1818 /label=BBa_B0010 terminator 1739..1818 /label=BBa_B0010 stem_loop 1834..1853 /label=T7 TE misc_feature 1860..1860 /label=stop polyA_site 1854..1867 /label=polya misc_feature 1827..1867 /label=BBa_B0012 terminator 1827..1867 /label=BBa_B0012 terminator 1739..1867 /label=BBa_B0015 misc_feature 1876..1895 /label=RhlR promoter 1894..1899 /label=-35 promoter 1917..1922 /label=-10 misc_feature 1928..1928 /label=start promoter 1876..1928 /label=BBa_R0071 RBS 1937..1951 /label=RBS-1\Strong RBS 1944..1948 /label=RBS misc_feature 1937..1951 /label=BBa_B0030 RBS 1937..1951 /label=BBa_B0030 CDS 1958..2686 /label=BBa_C0171 RBS 2695..2709 /label=RBS-1\Strong RBS 2702..2706 /label=RBS misc_feature 2695..2709 /label=BBa_B0030 RBS 2695..2709 /label=BBa_B0030 CDS 2719..3426 /label=cI lambda misc_feature 3427..3459 /label=LVA misc_feature 3466..3490 /label=Help:Barcodes CDS 2716..3490 /label=BBa_C0051 stem_loop 3510..3553 /label=stem_loop misc_feature 3499..3578 /label=BBa_B0010 terminator 3499..3578 /label=BBa_B0010 stem_loop 3594..3613 /label=T7 TE misc_feature 3620..3620 /label=stop polyA_site 3614..3627 /label=polya misc_feature 3587..3627 /label=BBa_B0012 terminator 3587..3627 /label=BBa_B0012 terminator 3499..3627 /label=BBa_B0015 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt 61 aaagaggaga aatactagat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 121 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 181 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 241 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 301 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 361 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 421 gactcctcct tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 481 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 541 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 601 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 661 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 721 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 781 tacaagtaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 841 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 901 accttcgggt gggcctttct gcgtttatat actagagttg acggctagct cagtcctagg 961 tacagtgcta gctactagag attaaagagg agaaatacta gatgaggaat gacggaggct 1021 ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc gatccacgac agccagggcg 1081 tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt cgattactac gcctatggcg 1141 tgcgccacac gattcccttc acccggccga agaccgaggt ccatggcacc tatcccaagg 1201 cctggctgga gcgataccag atgcagaact acggggccgt ggatccggcg atcctcaacg 1261 gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct gttcgaccag agccggatgc 1321 tctggaacga ggctcgcgat tggggcctct gtgtcggcgc gaccttgccg atccgcgcgc 1381 cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca gcagaacatc tccagcttcg 1441 agcgcgagga aatccgcctg cggctgcgtt gcatgatcga gttgctgacc cagaagctga 1501 ccgacctgga gcatccgatg ctgatgtcca acccggtctg cctgagccat cgcgaacgcg 1561 agatcctgca atggaccgcc gacggcaaga gttccgggga aatcgccatc atcctgagca 1621 tctccgagag cacggtgaac ttccaccaca agaacatcca gaagaagttc gacgcgccga 1681 acaagacgct ggctgccgcc tacgccgcgg cgctgggtct catctaataa tactagagcc 1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1861 gtttatatac tagagtcctg tgaaatctgg cagttaccgt tagctttcga attggctaaa 1921 aagtgttcta ctagagatta aagaggagaa atactagatg aggaatgacg gaggcttttt 1981 gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc agggcgtgtt 2041 cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct atggcgtgcg 2101 ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc ccaaggcctg 2161 gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc tcaacggcct 2221 gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc ggatgctctg 2281 gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc gcgcgccgaa 2341 caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca gcttcgagcg 2401 cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga agctgaccga 2461 cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg aacgcgagat 2521 cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc tgagcatctc 2581 cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg cgccgaacaa 2641 gacgctggct gccgcctacg ccgcggcgct gggtctcatc taataatact agagattaaa 2701 gaggagaaat actagatgag cacaaaaaag aaaccattaa cacaagagca gcttgaggac 2761 gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg aacttggctt atcccaggaa 2821 tctgtcgcag acaagatggg gatggggcag tcaggcgttg gtgctttatt taatggcatc 2881 aatgcattaa atgcttataa cgccgcattg cttgcaaaaa ttctcaaagt tagcgttgaa 2941 gaatttagcc cttcaatcgc cagagaaatc tacgagatgt atgaagcggt tagtatgcag 3001 ccgtcactta gaagtgagta tgagtaccct gttttttctc atgttcaggc agggatgttc 3061 tcacctgagc ttagaacctt taccaaaggt gatgcggaga gatgggtaag cacaaccaaa 3121 aaagccagtg attctgcatt ctggcttgag gttgaaggta attccatgac cgcaccaaca 3181 ggctccaagc caagctttcc tgacggaatg ttaattctcg ttgaccctga gcaggctgtt 3241 gagccaggtg atttctgcat agccagactt gggggtgatg agtttacctt caagaaactg 3301 atcagggata gcggtcaggt gtttttacaa ccactaaacc cacagtaccc aatgatccca 3361 tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta gtcagtggcc tgaagagacg 3421 tttggcgctg caaacgacga aaactacgct ttagtagctt aataacgctg atagtgctag 3481 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 3541 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 3601 cttcgggtgg gcctttctgc gtttata //