##gff-version 3
##sequence-region BBa_K1157013 1 3627
BBa_K1157013	.	terminator	3499	3627	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157013	.	terminator	3499	3578	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157013	.	engineered_region	3499	3578	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157013	.	stem_loop	3510	3553	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157013	.	terminator	3587	3627	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157013	.	stem_loop	3594	3613	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157013	.	stop_codon	3620	3620	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157013	.	engineered_region	3587	3627	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157013	.	polyA_site	3614	3627	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157013	.	CDS	1002	1730	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157013	.	promoter	938	972	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157013	.	engineered_region	79	792	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1157013	.	CDS	79	789	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1157013	.	sequence_alteration	430	430	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1157013	.	terminator	1739	1867	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157013	.	terminator	1739	1818	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157013	.	engineered_region	1739	1818	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157013	.	stem_loop	1750	1793	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157013	.	terminator	1827	1867	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157013	.	stem_loop	1834	1853	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157013	.	stop_codon	1860	1860	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157013	.	engineered_region	1827	1867	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157013	.	polyA_site	1854	1867	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157013	.	CDS	2716	3490	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K1157013	.	engineered_tag	3466	3490	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1157013	.	CDS	2719	3426	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1157013	.	tag	3427	3459	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1157013	.	terminator	801	929	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157013	.	terminator	801	880	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157013	.	engineered_region	801	880	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157013	.	stem_loop	812	855	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157013	.	terminator	889	929	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157013	.	stem_loop	896	915	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157013	.	stop_codon	922	922	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157013	.	engineered_region	889	929	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157013	.	polyA_site	916	929	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157013	.	ribosome_entry_site	1937	1951	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	1937	1951	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157013	.	engineered_region	1937	1951	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	1944	1948	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	981	995	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	981	995	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157013	.	engineered_region	981	995	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	988	992	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	2695	2709	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	2695	2709	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157013	.	engineered_region	2695	2709	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	2702	2706	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157013	.	promoter	1876	1928	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157013	.	promoter	1917	1922	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157013	.	start_codon	1928	1928	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157013	.	promoter	1894	1899	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157013	.	non_covalent_binding_site	1876	1895	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157013	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157013	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157013	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157013	.	CDS	1958	2686	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157013	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1157013	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1157013	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1157013	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1157013	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1157013	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
>BBa_K1157013
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgt
gaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtg
accaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagc
accccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgagga
cggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcacc
aacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgagg
acggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccac
ctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgag
gactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctag
ctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggaatgacggaggctttttgct
gtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtg
cggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatg
gcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcct
gcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattgg
ggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagc
agaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgac
cgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgcc
gacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatcc
agaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaatctggcagtta
ccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatgaggaatgacggaggc
tttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaa
aggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagaccga
ggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcctc
aacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggctc
gcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcg
cgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccag
aagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaat
ggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaa
gaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaataa
tactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacg
tcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatg
gggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattc
tcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagcc
gtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaaccttt
accaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggta
attccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgt
tgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcag
gtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcg
ctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtg
ctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata