##gff-version 3
##sequence-region BBa_K1157014 1 2727
BBa_K1157014	.	terminator	1667	1795	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157014	.	terminator	1667	1746	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157014	.	stem_loop	1678	1721	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157014	.	engineered_region	1667	1746	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157014	.	terminator	1755	1795	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157014	.	engineered_region	1755	1795	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157014	.	stem_loop	1762	1781	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157014	.	stop_codon	1788	1788	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157014	.	polyA_site	1782	1795	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157014	.	ribosome_entry_site	166	180	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	173	177	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157014	.	engineered_region	166	180	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	166	180	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157014	.	promoter	1	157	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K1157014	.	non_covalent_binding_site	106	125	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1157014	.	promoter	117	122	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1157014	.	promoter	140	145	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1157014	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1157014	.	terminator	2599	2727	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157014	.	terminator	2599	2678	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157014	.	stem_loop	2610	2653	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157014	.	engineered_region	2599	2678	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157014	.	terminator	2687	2727	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157014	.	engineered_region	2687	2727	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157014	.	stem_loop	2694	2713	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157014	.	stop_codon	2720	2720	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157014	.	polyA_site	2714	2727	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157014	.	ribosome_entry_site	918	932	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	925	929	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157014	.	engineered_region	918	932	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	918	932	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157014	.	CDS	939	1658	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1157014	.	CDS	939	1658	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1157014	.	CDS	1868	2590	.	+	0	ID=BBa_C0179;Name=BBa_C0179
BBa_K1157014	.	ribosome_entry_site	1847	1861	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	1854	1858	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157014	.	engineered_region	1847	1861	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157014	.	ribosome_entry_site	1847	1861	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157014	.	CDS	187	909	.	+	0	ID=BBa_C0179;Name=BBa_C0179
BBa_K1157014	.	promoter	1804	1838	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K1157014
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagct
actagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattg
gagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggaca
gccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgc
gcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcga
aagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcg
gcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgcc
gaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtg
gttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgca
actgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggc
cattatggccgttaatttgggtcttattactctctaataatactagagattaaagaggagaaatactagatgcgtaaag
gagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcag
tggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttcca
tggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgact
ttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacg
tgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaac
attcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatca
aagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaat
tggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaag
agagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctc
agtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagct
ggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctg
ttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagc
attacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctggga
accgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgacc
atgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaacc
gtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcga
acatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagt
tgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtg
tgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata