LasR-mCher
type: ComponentDefinition
persistent identity: BBa K1157014
display id: BBa_K1157014
version: 1
was generated by: iGEM to SBOL conversion
description: pLas-LasR-GFPmut3-Pc-LasR
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:30Z
mutable provenance: This is built by the original biobricks provided by igem official
owned by: james
owned by: myers
mutable description: This biosensor is built to sense C-12 AHL which originates from Pseudomonas aeruginosa. It uses GFP as a reporter, and constitutively express Rhl receptor. When binding with AHL molecules, Rhl receptor will regulate promoter sequence(pRhl) and express GFP which can be detected.
discontinued: false
dominant: false
experience: experience/None
group u list: _1469_
m user id: 0
owner id: 16435
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: These parts are built by igem official biobricks, so there is no big considerations of sequence design.
star: false
creator: Lin Ang Chieh
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_C0179
component: BBa_B0030
component: BBa_J23100
component: BBa_E0040
component: BBa_R0079
sequence annotation: BBa_R0079
sequence annotation: BBa_B0030
sequence annotation: BBa_C0179
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_J23100
sequence: https://synbiohub.org/public/igem/BBa_K1157014_sequence/1

BBa_B0015
type: Component
persistent identity: component2342983
display id: component2342983
version: 1
top level: LasR-mCher
definition: BBa_B0015
access: public

BBa_C0179
type: Component
persistent identity: component2342976
display id: component2342976
version: 1
top level: LasR-mCher
definition: lasR
access: public

BBa_B0030
type: Component
persistent identity: component2342974
display id: component2342974
version: 1
top level: LasR-mCher
definition: BBa_B0030
access: public

BBa_J23100
type: Component
persistent identity: component2342972
display id: component2342972
version: 1
top level: LasR-mCher
definition: BBa_J23100
access: public

BBa_B0015
type: Component
persistent identity: component2342971
display id: component2342971
version: 1
top level: LasR-mCher
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2342964
display id: component2342964
version: 1
top level: LasR-mCher
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2342961
display id: component2342961
version: 1
top level: LasR-mCher
definition: BBa_B0030
access: public

BBa_C0179
type: Component
persistent identity: component2342959
display id: component2342959
version: 1
top level: LasR-mCher
definition: lasR
access: public

BBa_B0030
type: Component
persistent identity: component2342957
display id: component2342957
version: 1
top level: LasR-mCher
definition: BBa_B0030
access: public

BBa_R0079
type: Component
persistent identity: component2342955
display id: component2342955
version: 1
top level: LasR-mCher
definition: LasR+PAI
access: public

BBa_R0079
type: SequenceAnnotation
persistent identity: annotation2342955
display id: annotation2342955
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342955/range2342955/1
component: BBa_R0079

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342955/range2342955/1
type: Range
persistent identity: range2342955
display id: range2342955
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 1
end: 157
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2342957
display id: annotation2342957
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342957/range2342957/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342957/range2342957/1
type: Range
persistent identity: range2342957
display id: range2342957
version: 1
top level: LasR-mCher
direction: direction/forward
start: 166
end: 180
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2342959
display id: annotation2342959
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342959/range2342959/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342959/range2342959/1
type: Range
persistent identity: range2342959
display id: range2342959
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 187
end: 909
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2342961
display id: annotation2342961
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342961/range2342961/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342961/range2342961/1
type: Range
persistent identity: range2342961
display id: range2342961
version: 1
top level: LasR-mCher
direction: direction/forward
start: 918
end: 932
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2342964
display id: annotation2342964
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342964/range2342964/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342964/range2342964/1
type: Range
persistent identity: range2342964
display id: range2342964
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 939
end: 1658
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2342971
display id: annotation2342971
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342971/range2342971/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342971/range2342971/1
type: Range
persistent identity: range2342971
display id: range2342971
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 1667
end: 1795
orientation: inline

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2342972
display id: annotation2342972
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342972/range2342972/1
component: BBa_J23100

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342972/range2342972/1
type: Range
persistent identity: range2342972
display id: range2342972
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 1804
end: 1838
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2342974
display id: annotation2342974
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342974/range2342974/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342974/range2342974/1
type: Range
persistent identity: range2342974
display id: range2342974
version: 1
top level: LasR-mCher
direction: direction/forward
start: 1847
end: 1861
orientation: inline

BBa_C0179
type: SequenceAnnotation
persistent identity: annotation2342976
display id: annotation2342976
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342976/range2342976/1
component: BBa_C0179

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342976/range2342976/1
type: Range
persistent identity: range2342976
display id: range2342976
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 1868
end: 2590
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2342983
display id: annotation2342983
version: 1
top level: LasR-mCher
location: https://synbiohub.org/public/igem/BBa_K1157014/annotation2342983/range2342983/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1157014/annotation2342983/range2342983/1
type: Range
persistent identity: range2342983
display id: range2342983
version: 1
top level: LasR-mCher
direction: direction/reverse
start: 2599
end: 2727
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

LasR+PAI
type: ComponentDefinition
persistent identity: BBa R0079
display id: BBa_R0079
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (LasR & PAI regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: "Analysis of the Pseudomonas aeruginosa Elastase (lasB) Regulatory Region". Lynn Rust, Everett Pesci, and Barbara Iglewski
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Binding region for LasR protein (positive regulation)
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: OP2
sequence annotation: OP1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0079_sequence/1

OP2
type: SequenceAnnotation
persistent identity: annotation300985
display id: annotation300985
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300985/range300985/1
type: Range
persistent identity: range300985
display id: range300985
version: 1
top level: LasR+PAI
direction: direction/forward
start: 46
end: 63
orientation: inline

OP1
type: SequenceAnnotation
persistent identity: annotation300992
display id: annotation300992
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0079/annotation300992/range300992/1
type: Range
persistent identity: range300992
display id: range300992
version: 1
top level: LasR+PAI
direction: direction/forward
start: 106
end: 125
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318495
display id: annotation318495
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0079/annotation318495/range318495/1
type: Range
persistent identity: range318495
display id: range318495
version: 1
top level: LasR+PAI
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318496
display id: annotation318496
version: 1
top level: LasR+PAI
location: https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0079/annotation318496/range318496/1
type: Range
persistent identity: range318496
display id: range318496
version: 1
top level: LasR+PAI
direction: direction/forward
start: 140
end: 145
orientation: inline

BBa_J23100
type: ComponentDefinition
persistent identity: BBa J23100
display id: BBa_J23100
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23100_sequence/1

lasR
type: ComponentDefinition
persistent identity: BBa C0179
display id: BBa_C0179
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(no LVA)
created: 2004-05-26T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: same as C0079 except no LVA tag
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_C0179_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1157014_sequence/1
type: Sequence
persistent identity: BBa K1157014 sequence
display id: BBa_K1157014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0079_sequence/1
type: Sequence
persistent identity: BBa R0079 sequence
display id: BBa_R0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23100_sequence/1
type: Sequence
persistent identity: BBa J23100 sequence
display id: BBa_J23100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0179_sequence/1
type: Sequence
persistent identity: BBa C0179 sequence
display id: BBa_C0179_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z