LOCUS BBa_K1157015 3627 bp DNA linear 25-Nov-2024 DEFINITION pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry ACCESSION BBa_K1157015 VERSION BBa_K1157015.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 CDS 65..793 /label=BBa_C0171 stem_loop 813..856 /label=stem_loop misc_feature 802..881 /label=BBa_B0010 terminator 802..881 /label=BBa_B0010 stem_loop 897..916 /label=T7 TE misc_feature 923..923 /label=stop polyA_site 917..930 /label=polya misc_feature 890..930 /label=BBa_B0012 terminator 890..930 /label=BBa_B0012 terminator 802..930 /label=BBa_B0015 misc_feature 939..958 /label=RhlR promoter 957..962 /label=-35 promoter 980..985 /label=-10 misc_feature 991..991 /label=start promoter 939..991 /label=BBa_R0071 RBS 1000..1014 /label=RBS-1\Strong RBS 1007..1011 /label=RBS misc_feature 1000..1014 /label=BBa_B0030 RBS 1000..1014 /label=BBa_B0030 CDS 1021..1749 /label=BBa_C0171 RBS 1758..1772 /label=RBS-1\Strong RBS 1765..1769 /label=RBS misc_feature 1758..1772 /label=BBa_B0030 RBS 1758..1772 /label=BBa_B0030 CDS 1782..2489 /label=cI lambda misc_feature 2490..2522 /label=LVA misc_feature 2529..2553 /label=Help:Barcodes CDS 1779..2553 /label=BBa_C0051 stem_loop 2573..2616 /label=stem_loop misc_feature 2562..2641 /label=BBa_B0010 terminator 2562..2641 /label=BBa_B0010 stem_loop 2657..2676 /label=T7 TE misc_feature 2683..2683 /label=stop polyA_site 2677..2690 /label=polya misc_feature 2650..2690 /label=BBa_B0012 terminator 2650..2690 /label=BBa_B0012 terminator 2562..2690 /label=BBa_B0015 promoter 2736..2741 /label=-10 promoter 2713..2718 /label=-35 misc_feature 2723..2739 /label=OR1 misc_feature 2699..2715 /label=OR2 misc_feature 2699..2747 /label=BBa_R0051 promoter 2699..2747 /label=BBa_R0051 RBS 2756..2770 /label=RBS-1\Strong RBS 2763..2767 /label=RBS misc_feature 2756..2770 /label=BBa_B0030 RBS 2756..2770 /label=BBa_B0030 CDS 2777..3487 /label=mCherry misc_feature 3128..3128 /label=C->T (removing PstI site) misc_feature 2777..3490 /label=BBa_J06504 stem_loop 3510..3553 /label=stem_loop misc_feature 3499..3578 /label=BBa_B0010 terminator 3499..3578 /label=BBa_B0010 stem_loop 3594..3613 /label=T7 TE misc_feature 3620..3620 /label=stop polyA_site 3614..3627 /label=polya misc_feature 3587..3627 /label=BBa_B0012 terminator 3587..3627 /label=BBa_B0012 terminator 3499..3627 /label=BBa_B0015 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata 61 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca 121 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg 181 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga 241 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc 301 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag 361 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg 421 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga 481 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat 541 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt 601 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg 661 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat 721 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg 781 tctcatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 901 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac 961 cgttagcttt cgaattggct aaaaagtgtt ctactagaga ttaaagagga gaaatactag 1021 atgaggaatg acggaggctt tttgctgtgg tgggacggtt tgcgtagcga gatgcagccg 1081 atccacgaca gccagggcgt gttcgccgtc ctggaaaagg aagtgcggcg cctgggcttc 1141 gattactacg cctatggcgt gcgccacacg attcccttca cccggccgaa gaccgaggtc 1201 catggcacct atcccaaggc ctggctggag cgataccaga tgcagaacta cggggccgtg 1261 gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg tggtctggag cgacagcctg 1321 ttcgaccaga gccggatgct ctggaacgag gctcgcgatt ggggcctctg tgtcggcgcg 1381 accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc tttccgtggc gcgcgaccag 1441 cagaacatct ccagcttcga gcgcgaggaa atccgcctgc ggctgcgttg catgatcgag 1501 ttgctgaccc agaagctgac cgacctggag catccgatgc tgatgtccaa cccggtctgc 1561 ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg acggcaagag ttccggggaa 1621 atcgccatca tcctgagcat ctccgagagc acggtgaact tccaccacaa gaacatccag 1681 aagaagttcg acgcgccgaa caagacgctg gctgccgcct acgccgcggc gctgggtctc 1741 atctaataat actagagatt aaagaggaga aatactagat gagcacaaaa aagaaaccat 1801 taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa 1861 atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg 1921 ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa 1981 aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga 2041 tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt 2101 ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg 2161 agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag 2221 gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc 2281 tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg 2341 atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa 2401 acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg 2461 ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag 2521 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg 2581 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 2641 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta 2701 acaccgtgcg tgttgactat tttacctctg gcggtgataa tggttgctac tagagattaa 2761 agaggagaaa tactagatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 2821 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 2881 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 2941 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 3001 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 3061 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 3121 ctcctccttg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 3181 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 3241 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 3301 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 3361 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 3421 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 3481 caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 3541 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 3601 cttcgggtgg gcctttctgc gtttata //