##gff-version 3
##sequence-region BBa_K1157015 1 3627
BBa_K1157015	.	promoter	939	991	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157015	.	promoter	957	962	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157015	.	promoter	980	985	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157015	.	non_covalent_binding_site	939	958	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157015	.	start_codon	991	991	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157015	.	terminator	802	930	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157015	.	terminator	890	930	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157015	.	polyA_site	917	930	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157015	.	stem_loop	897	916	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157015	.	stop_codon	923	923	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157015	.	engineered_region	890	930	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157015	.	terminator	802	881	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157015	.	stem_loop	813	856	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157015	.	engineered_region	802	881	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157015	.	terminator	2562	2690	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157015	.	terminator	2650	2690	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157015	.	polyA_site	2677	2690	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157015	.	stem_loop	2657	2676	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157015	.	stop_codon	2683	2683	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157015	.	engineered_region	2650	2690	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157015	.	terminator	2562	2641	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157015	.	stem_loop	2573	2616	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157015	.	engineered_region	2562	2641	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157015	.	ribosome_entry_site	2756	2770	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	2756	2770	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	2763	2767	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157015	.	engineered_region	2756	2770	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157015	.	terminator	3499	3627	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157015	.	terminator	3587	3627	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157015	.	polyA_site	3614	3627	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157015	.	stem_loop	3594	3613	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157015	.	stop_codon	3620	3620	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157015	.	engineered_region	3587	3627	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157015	.	terminator	3499	3578	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157015	.	stem_loop	3510	3553	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157015	.	engineered_region	3499	3578	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157015	.	promoter	2699	2747	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1157015	.	engineered_region	2699	2747	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1157015	.	non_covalent_binding_site	2723	2739	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1157015	.	non_covalent_binding_site	2699	2715	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1157015	.	promoter	2736	2741	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1157015	.	promoter	2713	2718	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1157015	.	CDS	1779	2553	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K1157015	.	CDS	1782	2489	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1157015	.	tag	2490	2522	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1157015	.	engineered_tag	2529	2553	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1157015	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157015	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157015	.	CDS	65	793	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157015	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157015	.	CDS	1021	1749	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157015	.	ribosome_entry_site	1758	1772	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	1758	1772	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	1765	1769	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157015	.	engineered_region	1758	1772	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157015	.	engineered_region	2777	3490	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1157015	.	CDS	2777	3487	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1157015	.	sequence_alteration	3128	3128	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1157015	.	ribosome_entry_site	1000	1014	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	1000	1014	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157015	.	ribosome_entry_site	1007	1011	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157015	.	engineered_region	1000	1014	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_K1157015
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatgaggaatgacgga
ggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgg
aaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagac
cgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatc
ctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgagg
ctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggc
gcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacc
cagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgc
aatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccacca
caagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatctaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcctgtgaaa
tctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatactagatgagga
atgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgc
cgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccgg
ccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatc
cggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctg
gaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctt
tccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagt
tgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcga
gatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaac
ttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtc
tcatctaataatactagagattaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagctt
gaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagaca
agatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgct
tgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggtt
agtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagc
ttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttga
ggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccct
gagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcaggg
atagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggg
gaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataa
cgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagagg
agaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacat
ggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgcc
aagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaagg
cctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgat
gaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaag
ctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcgga
tgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctga
ggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacc
tcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagc
tgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata