##gff-version 3
##sequence-region BBa_K1157016 1 1960
BBa_K1157016	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157016	.	CDS	1119	1823	.	+	0	ID=BBa_K592100;Name=BBa_K592100
BBa_K1157016	.	CDS	1119	1817	.	+	0	ID=annotation2135490;Name=mTagBFP;Parent=BBa_K592100
BBa_K1157016	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157016	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157016	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157016	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157016	.	terminator	1832	1960	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157016	.	terminator	1832	1911	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157016	.	engineered_region	1832	1911	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157016	.	stem_loop	1843	1886	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157016	.	terminator	1920	1960	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157016	.	stem_loop	1927	1946	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157016	.	stop_codon	1953	1953	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157016	.	engineered_region	1920	1960	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157016	.	polyA_site	1947	1960	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157016	.	promoter	933	1089	.	+	0	ID=BBa_R0079;Name=BBa_R0079
BBa_K1157016	.	promoter	1072	1077	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K1157016	.	non_covalent_binding_site	978	995	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K1157016	.	non_covalent_binding_site	1038	1057	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K1157016	.	promoter	1049	1054	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K1157016	.	CDS	65	787	.	+	0	ID=BBa_C0179;Name=BBa_C0179
BBa_K1157016	.	terminator	796	924	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157016	.	terminator	796	875	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157016	.	engineered_region	796	875	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157016	.	stem_loop	807	850	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157016	.	terminator	884	924	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157016	.	stem_loop	891	910	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157016	.	stop_codon	917	917	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157016	.	engineered_region	884	924	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157016	.	polyA_site	911	924	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157016	.	ribosome_entry_site	1098	1112	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157016	.	ribosome_entry_site	1098	1112	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157016	.	engineered_region	1098	1112	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157016	.	ribosome_entry_site	1105	1109	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_K1157016
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagatggccttggttgac
ggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggat
tctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggc
cgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactg
ccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctgg
tgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccg
ggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgcc
ggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgcca
tcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcg
gcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctctaataatac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgc
gtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcga
aatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagattaaagag
gagaaatactagatgagcgaactgatcaaagagaacatgcacatgaagctgtacatggaaggcaccgttgacaaccacc
actttaagtgcacgtctgagggtgagggtaagccgtacgaaggcacccaaaccatgcgtatcaaagttgtggagggcgg
tccactgccgttcgcttttgacattctggcgaccagcttcctgtacggttccaaaacgttcattaaccatactcagggc
attccggatttcttcaaacagagctttccggaaggtttcacctgggagcgtgtcaccacgtatgaagatggtggtgtgt
tgaccgccacccaagatacctccctgcaagatggctgtctgatctataacgtgaaaattcgtggcgtcaactttacgag
caatggtccggtgatgcagaagaaaaccctgggttgggaggcgtttacggaaaccctgtatccggccgatggtggcctg
gagggccgtaacgacatggcactgaagctggttggtggcagccatttgatcgcaaatatcaagacgacgtaccgcagca
agaaaccggcgaaaaatctgaagatgccgggtgtttactatgtcgactaccgtctggaacgcattaaagaagcgaataa
tgagacttacgtggagcagcacgaggttgcagtcgcgcgctattgcgacttgcctagcaagctgggtcataaactgaat
taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg
tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata