LOCUS       BBa_K1157017            1227 bp    DNA     linear       06-Dec-2025
DEFINITION  pPQS-GFPmut3
ACCESSION   BBa_K1157017
VERSION     BBa_K1157017.1  
FEATURES             Location/Qualifiers
     promoter        1..341
                     /label=BBa_K1157000
     RBS             350..364
                     /label=RBS-1\Strong
     RBS             357..361
                     /label=RBS
     misc_feature    350..364
                     /label=BBa_B0030
     RBS             350..364
                     /label=BBa_B0030
     CDS             371..1090
                     /label=GFP protein
     CDS             371..1090
                     /label=BBa_E0040
     stem_loop       1110..1153
                     /label=stem_loop
     misc_feature    1099..1178
                     /label=BBa_B0010
     terminator      1099..1178
                     /label=BBa_B0010
     stem_loop       1194..1213
                     /label=T7 TE
     misc_feature    1220..1220
                     /label=stop
     polyA_site      1214..1227
                     /label=polya
     misc_feature    1187..1227
                     /label=BBa_B0012
     terminator      1187..1227
                     /label=BBa_B0012
     terminator      1099..1227
                     /label=BBa_B0015
ORIGIN
        1 gggtgtgcca aatttctcgc ggtttggttc gcgccgattg ccgcggccta cgaagcccgt
       61 ggttcttctc cccgaaactt tttcgttcgg actccgaata tcgcgcttcg cccagcgccg
      121 ctagtttccc gttcctgaca aagcaagcgc tctggctcag gtatctcctg atccggatgc
      181 atatcgctga agagggaacg ttctgtcatg tccacattgg ccaacctgac cgaggttctg
      241 ttccgcctcg atttcgatcc cgataccgcc gtttatcact atcggggcca gactctcagc
      301 cggctgcaat gccggaccta cattctctcc caggccagcc atactagaga ttaaagagga
      361 gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      421 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      481 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      541 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      601 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      661 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      721 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      781 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      841 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      901 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      961 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     1021 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     1081 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1141 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1201 cttcgggtgg gcctttctgc gtttata
//