BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K1157000
1
pPQS
promoter sequence for PQS quorum sensing
2013-08-22T11:00:00Z
2015-05-08T01:09:30Z
It comes from Psuedomonas Aeruginosa strain ATCC 27853 genomic sequence.
This is a quorum sensing promoter sequence for PQS transcriptional regulator.
The sequence is a 391 bp sequence that is cloned from Psuedomonas Aeruginosa strain ATCC 27853.
It is similar to the LuxR-pLux system, and can be used to sense quorum sensing molecules.
It is added into one of our constructs for quorum sensing biosensors that is targeted for 2-heptyl-3,4-dihydroxyquinoline.
false
false
_1469_
0
16435
9
In stock
false
This is almost the identical sequence as the genome in the bacterium.
false
Lin Ang Chieh
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1157017
1
pPQS-GFP
pPQS-GFPmut3
2013-09-14T11:00:00Z
2015-05-08T01:09:30Z
pPQS is originally made through PCR of whole genome Pseudomonas aeruginosa ATCC 27853. The reporter is constructed by igem biobricks from iGEM official.
This is a sub-part of the quorum sensing molecule 2-heptyl-3-hydroxy-4-quinolone. It is composed by a promoter sequence of PQS and a reporter GFPmut3.
false
false
_1469_
0
16435
9
In stock
false
There are no restriction sites in the inner part of the DNA so there are no specific design to the sequence.
false
Lin Ang Chieh
component2343096
1
BBa_K1157000
component2343098
1
BBa_B0030
component2343108
1
BBa_B0015
component2343101
1
BBa_E0040
annotation2343101
1
BBa_E0040
range2343101
1
371
1090
annotation2343096
1
BBa_K1157000
range2343096
1
1
341
annotation2343098
1
BBa_B0030
range2343098
1
350
364
annotation2343108
1
BBa_B0015
range2343108
1
1099
1227
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1157000_sequence
1
gggtgtgccaaatttctcgcggtttggttcgcgccgattgccgcggcctacgaagcccgtggttcttctccccgaaactttttcgttcggactccgaatatcgcgcttcgcccagcgccgctagtttcccgttcctgacaaagcaagcgctctggctcaggtatctcctgatccggatgcatatcgctgaagagggaacgttctgtcatgtccacattggccaacctgaccgaggttctgttccgcctcgatttcgatcccgataccgccgtttatcactatcggggccagactctcagccggctgcaatgccggacctacattctctcccaggccagcca
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1157017_sequence
1
gggtgtgccaaatttctcgcggtttggttcgcgccgattgccgcggcctacgaagcccgtggttcttctccccgaaactttttcgttcggactccgaatatcgcgcttcgcccagcgccgctagtttcccgttcctgacaaagcaagcgctctggctcaggtatctcctgatccggatgcatatcgctgaagagggaacgttctgtcatgtccacattggccaacctgaccgaggttctgttccgcctcgatttcgatcccgataccgccgtttatcactatcggggccagactctcagccggctgcaatgccggacctacattctctcccaggccagccatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z