##gff-version 3
##sequence-region BBa_K1157018 1 2629
BBa_K1157018	.	CDS	1764	2492	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157018	.	ribosome_entry_site	820	834	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	820	834	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	827	831	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157018	.	engineered_region	820	834	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157018	.	terminator	1563	1691	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157018	.	terminator	1651	1691	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157018	.	polyA_site	1678	1691	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157018	.	engineered_region	1651	1691	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157018	.	stem_loop	1658	1677	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157018	.	stop_codon	1684	1684	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157018	.	terminator	1563	1642	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157018	.	stem_loop	1574	1617	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157018	.	engineered_region	1563	1642	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157018	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157018	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157018	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157018	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157018	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157018	.	engineered_region	841	1554	.	+	0	ID=BBa_J06504;Name=BBa_J06504
BBa_K1157018	.	sequence_alteration	1192	1192	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K1157018	.	CDS	841	1551	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K1157018	.	ribosome_entry_site	1743	1757	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	1743	1757	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	1750	1754	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157018	.	engineered_region	1743	1757	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157018	.	promoter	1700	1734	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157018	.	ribosome_entry_site	62	76	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	62	76	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157018	.	ribosome_entry_site	69	73	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157018	.	engineered_region	62	76	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157018	.	CDS	83	811	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157018	.	terminator	2501	2629	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157018	.	terminator	2589	2629	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157018	.	polyA_site	2616	2629	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157018	.	engineered_region	2589	2629	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157018	.	stem_loop	2596	2615	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157018	.	stop_codon	2622	2622	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157018	.	terminator	2501	2580	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157018	.	stem_loop	2512	2555	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157018	.	engineered_region	2501	2580	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1157018
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatac
tagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagg
gcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcc
cttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggg
gccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagcc
ggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgct
cagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgc
atgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatc
gcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagag
cacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcg
gcgctgggtctcatctaataatactagagattaaagaggagaaatactagatggtgagcaagggcgaggaggataacat
ggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggc
gagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcct
gggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaa
gctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggac
tcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgc
agaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagca
gaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctg
cccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaac
gcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgctagctact
agagattaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatg
cagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcct
atggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcg
ataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagc
gacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccga
tccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgagga
aatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtcc
aacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgcca
tcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagac
gctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata