BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K1157019
1
Pc-PQSR
Pc-PQSR
2013-09-14T11:00:00Z
2015-05-08T01:09:30Z
PQSR is a pcr product of Pseudomonas aeruginosa ATCC 27853. The rest is provided by iGEM official biobrick.
This is a sub-part of quorum sensing molecule biosensor 2-heptyl-3-hydroxy-4(1H)-quinolone sensed by PQS receptor. The constitutive promoter expresses PQS receptor, and in the final construct, if the PQS receptor(PQSR) binds with 2-heptyl-3-hydroxy-4(1H)-quinolone, it will be able to regulate its promoter sequence resulting in expression of reporter mCherry.
false
false
_1469_
0
16435
9
It's complicated
false
There are no restriction sites inside the sequence.
false
Lin Ang Chieh
component2343149
1
BBa_K1157001
component2343147
1
BBa_B0030
component2343156
1
BBa_B0015
component2343145
1
BBa_J23119
annotation2343145
1
BBa_J23119
range2343145
1
1
35
annotation2343147
1
BBa_B0030
range2343147
1
44
58
annotation2343156
1
BBa_B0015
range2343156
1
1072
1200
annotation2343149
1
BBa_K1157001
range2343149
1
65
1063
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_K1157001
1
PQSR
PQSR
2013-08-27T11:00:00Z
2015-05-08T01:09:30Z
It comes from Pseudomonas ATCC 27853 genome, functioning as one of the quorum sensing systems in the bacterium.
PQSR is a quorum sensing receptor which is similar to the LuxI-LuxR system. It is found in Pseudomonas and uses 2-heptyl-3-hydroxy-4-quinolone as quorum sensing molecule. We use it as one of our quorum sensing receptors and hope to function as a biosensor.
false
false
_1469_
0
16435
9
It's complicated
false
There are no PstI, EcoRI, XbaI or SpeI restriction sites in the sequence.
false
Lin Ang Chieh
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1157001_sequence
1
atgcctattcataacctgaatcacgtgaacatgttcctccaggtcatcgcctccggttcgatttcctccgctgcgcggatcctgcgcaagtcgcacaccgcggtcagctcggcggtcagcaacctggaaatcgacctgtgcgtggagctggtccgtcgggacggctacaaggtcgaacccaccgagcaggcgcttcgcctgatcccttacatgcgcagcctgctgaactaccagcagctgatcggcgacatcgccttcaatctcaacaagggtccgcgcaatctccgggtgctgctggacaccgccatcccgccgtcgttctgcgatacggtgagcagcgtactgctcgacgatttcaacatggtcagcctgatacgcacctcgcccgccgatagcctggcgacgatcaagcaggacaacgcggaaatcgatatcgccatcaccatcgacgaggaactgaagatctcccgcttcaaccagtgcgtgctcggctacaccaaggcgttcgtcgtcgcccatccgcagcacccgttgtgcaatgcctccctgcacagcatcgcgagcctggccaattaccggcagatcagcctcggcagccgctccgggcagcattcgaacctgctgcggccggtcagcgacaaggtgctcttcgtggaaaacttcgacgacatgctgcgtctggtggaagccggcgtcggatggggcatcgcgccgcattatttcgtcgaggaacgcctgcgcaacggtaccctggcagtcctcagcgaactctacgaaccgggcggcatcgacaccaaggtgtattgctactacaacaccgcgctggaatccgagcgcagcttcctgcgctttctcgaaagcgcccgccagcgcctgcgcgaactgggccgccagcgtttcgacgatgcgccggcctggcaaccgagcatcgtcgaaacggtgcagcgccgctcaggcccgaaggcgctcgcgtaccgccagcgcgccgcaccagagtag
BBa_K1157019_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgcctattcataacctgaatcacgtgaacatgttcctccaggtcatcgcctccggttcgatttcctccgctgcgcggatcctgcgcaagtcgcacaccgcggtcagctcggcggtcagcaacctggaaatcgacctgtgcgtggagctggtccgtcgggacggctacaaggtcgaacccaccgagcaggcgcttcgcctgatcccttacatgcgcagcctgctgaactaccagcagctgatcggcgacatcgccttcaatctcaacaagggtccgcgcaatctccgggtgctgctggacaccgccatcccgccgtcgttctgcgatacggtgagcagcgtactgctcgacgatttcaacatggtcagcctgatacgcacctcgcccgccgatagcctggcgacgatcaagcaggacaacgcggaaatcgatatcgccatcaccatcgacgaggaactgaagatctcccgcttcaaccagtgcgtgctcggctacaccaaggcgttcgtcgtcgcccatccgcagcacccgttgtgcaatgcctccctgcacagcatcgcgagcctggccaattaccggcagatcagcctcggcagccgctccgggcagcattcgaacctgctgcggccggtcagcgacaaggtgctcttcgtggaaaacttcgacgacatgctgcgtctggtggaagccggcgtcggatggggcatcgcgccgcattatttcgtcgaggaacgcctgcgcaacggtaccctggcagtcctcagcgaactctacgaaccgggcggcatcgacaccaaggtgtattgctactacaacaccgcgctggaatccgagcgcagcttcctgcgctttctcgaaagcgcccgccagcgcctgcgcgaactgggccgccagcgtttcgacgatgcgccggcctggcaaccgagcatcgtcgaaacggtgcagcgccgctcaggcccgaaggcgctcgcgtaccgccagcgcgccgcaccagagtagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z