LOCUS       BBa_K1157020            2635 bp    DNA     linear       30-Dec-2025
DEFINITION  Pc-RhlR-pRhl-RhlR-GFPmut3
ACCESSION   BBa_K1157020
VERSION     BBa_K1157020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..793
                     /label=BBa_C0171
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
     terminator      802..930
                     /label=BBa_B0015
     misc_feature    939..958
                     /label=RhlR
     promoter        957..962
                     /label=-35
     promoter        980..985
                     /label=-10
     misc_feature    991..991
                     /label=start
     promoter        939..991
                     /label=BBa_R0071
     RBS             1000..1014
                     /label=RBS-1\Strong
     RBS             1007..1011
                     /label=RBS
     misc_feature    1000..1014
                     /label=BBa_B0030
     RBS             1000..1014
                     /label=BBa_B0030
     CDS             1021..1749
                     /label=BBa_C0171
     RBS             1758..1772
                     /label=RBS-1\Strong
     RBS             1765..1769
                     /label=RBS
     misc_feature    1758..1772
                     /label=BBa_B0030
     RBS             1758..1772
                     /label=BBa_B0030
     CDS             1779..2498
                     /label=GFP protein
     CDS             1779..2498
                     /label=BBa_E0040
     stem_loop       2518..2561
                     /label=stem_loop
     misc_feature    2507..2586
                     /label=BBa_B0010
     terminator      2507..2586
                     /label=BBa_B0010
     stem_loop       2602..2621
                     /label=T7 TE
     misc_feature    2628..2628
                     /label=stop
     polyA_site      2622..2635
                     /label=polya
     misc_feature    2595..2635
                     /label=BBa_B0012
     terminator      2595..2635
                     /label=BBa_B0012
     terminator      2507..2635
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagattaaag aggagaaata
       61 ctagatgagg aatgacggag gctttttgct gtggtgggac ggtttgcgta gcgagatgca
      121 gccgatccac gacagccagg gcgtgttcgc cgtcctggaa aaggaagtgc ggcgcctggg
      181 cttcgattac tacgcctatg gcgtgcgcca cacgattccc ttcacccggc cgaagaccga
      241 ggtccatggc acctatccca aggcctggct ggagcgatac cagatgcaga actacggggc
      301 cgtggatccg gcgatcctca acggcctgcg ctcctcggaa atggtggtct ggagcgacag
      361 cctgttcgac cagagccgga tgctctggaa cgaggctcgc gattggggcc tctgtgtcgg
      421 cgcgaccttg ccgatccgcg cgccgaacaa tttgctcagc gtgctttccg tggcgcgcga
      481 ccagcagaac atctccagct tcgagcgcga ggaaatccgc ctgcggctgc gttgcatgat
      541 cgagttgctg acccagaagc tgaccgacct ggagcatccg atgctgatgt ccaacccggt
      601 ctgcctgagc catcgcgaac gcgagatcct gcaatggacc gccgacggca agagttccgg
      661 ggaaatcgcc atcatcctga gcatctccga gagcacggtg aacttccacc acaagaacat
      721 ccagaagaag ttcgacgcgc cgaacaagac gctggctgcc gcctacgccg cggcgctggg
      781 tctcatctaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata tactagagtc ctgtgaaatc tggcagttac
      961 cgttagcttt cgaattggct aaaaagtgtt ctactagaga ttaaagagga gaaatactag
     1021 atgaggaatg acggaggctt tttgctgtgg tgggacggtt tgcgtagcga gatgcagccg
     1081 atccacgaca gccagggcgt gttcgccgtc ctggaaaagg aagtgcggcg cctgggcttc
     1141 gattactacg cctatggcgt gcgccacacg attcccttca cccggccgaa gaccgaggtc
     1201 catggcacct atcccaaggc ctggctggag cgataccaga tgcagaacta cggggccgtg
     1261 gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg tggtctggag cgacagcctg
     1321 ttcgaccaga gccggatgct ctggaacgag gctcgcgatt ggggcctctg tgtcggcgcg
     1381 accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc tttccgtggc gcgcgaccag
     1441 cagaacatct ccagcttcga gcgcgaggaa atccgcctgc ggctgcgttg catgatcgag
     1501 ttgctgaccc agaagctgac cgacctggag catccgatgc tgatgtccaa cccggtctgc
     1561 ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg acggcaagag ttccggggaa
     1621 atcgccatca tcctgagcat ctccgagagc acggtgaact tccaccacaa gaacatccag
     1681 aagaagttcg acgcgccgaa caagacgctg gctgccgcct acgccgcggc gctgggtctc
     1741 atctaataat actagagatt aaagaggaga aatactagat gcgtaaagga gaagaacttt
     1801 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     1861 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
     1921 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg
     1981 ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca
     2041 tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga
     2101 cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta
     2161 ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
     2221 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta
     2281 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     2341 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     2401 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     2461 ggattacaca tggcatggat gaactataca aataataata ctagagccag gcatcaaata
     2521 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2581 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
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