LOCUS       BBa_K1157021            2635 bp    DNA     linear       26-Dec-2024
DEFINITION  pRhl-RhlR-GFP-Pc-RhlR
ACCESSION   BBa_K1157021
VERSION     BBa_K1157021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=RhlR
     promoter        19..24
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     promoter        1..53
                     /label=BBa_R0071
     RBS             62..76
                     /label=RBS-1\Strong
     RBS             69..73
                     /label=RBS
     misc_feature    62..76
                     /label=BBa_B0030
     RBS             62..76
                     /label=BBa_B0030
     CDS             83..811
                     /label=BBa_C0171
     RBS             820..834
                     /label=RBS-1\Strong
     RBS             827..831
                     /label=RBS
     misc_feature    820..834
                     /label=BBa_B0030
     RBS             820..834
                     /label=BBa_B0030
     CDS             841..1560
                     /label=GFP protein
     CDS             841..1560
                     /label=BBa_E0040
     stem_loop       1580..1623
                     /label=stem_loop
     misc_feature    1569..1648
                     /label=BBa_B0010
     terminator      1569..1648
                     /label=BBa_B0010
     stem_loop       1664..1683
                     /label=T7 TE
     misc_feature    1690..1690
                     /label=stop
     polyA_site      1684..1697
                     /label=polya
     misc_feature    1657..1697
                     /label=BBa_B0012
     terminator      1657..1697
                     /label=BBa_B0012
     terminator      1569..1697
                     /label=BBa_B0015
     promoter        1706..1740
                     /label=BBa_J23100
     RBS             1749..1763
                     /label=RBS-1\Strong
     RBS             1756..1760
                     /label=RBS
     misc_feature    1749..1763
                     /label=BBa_B0030
     RBS             1749..1763
                     /label=BBa_B0030
     CDS             1770..2498
                     /label=BBa_C0171
     stem_loop       2518..2561
                     /label=stem_loop
     misc_feature    2507..2586
                     /label=BBa_B0010
     terminator      2507..2586
                     /label=BBa_B0010
     stem_loop       2602..2621
                     /label=T7 TE
     misc_feature    2628..2628
                     /label=stop
     polyA_site      2622..2635
                     /label=polya
     misc_feature    2595..2635
                     /label=BBa_B0012
     terminator      2595..2635
                     /label=BBa_B0012
     terminator      2507..2635
                     /label=BBa_B0015
ORIGIN
        1 tcctgtgaaa tctggcagtt accgttagct ttcgaattgg ctaaaaagtg ttctactaga
       61 gattaaagag gagaaatact agatgaggaa tgacggaggc tttttgctgt ggtgggacgg
      121 tttgcgtagc gagatgcagc cgatccacga cagccagggc gtgttcgccg tcctggaaaa
      181 ggaagtgcgg cgcctgggct tcgattacta cgcctatggc gtgcgccaca cgattccctt
      241 cacccggccg aagaccgagg tccatggcac ctatcccaag gcctggctgg agcgatacca
      301 gatgcagaac tacggggccg tggatccggc gatcctcaac ggcctgcgct cctcggaaat
      361 ggtggtctgg agcgacagcc tgttcgacca gagccggatg ctctggaacg aggctcgcga
      421 ttggggcctc tgtgtcggcg cgaccttgcc gatccgcgcg ccgaacaatt tgctcagcgt
      481 gctttccgtg gcgcgcgacc agcagaacat ctccagcttc gagcgcgagg aaatccgcct
      541 gcggctgcgt tgcatgatcg agttgctgac ccagaagctg accgacctgg agcatccgat
      601 gctgatgtcc aacccggtct gcctgagcca tcgcgaacgc gagatcctgc aatggaccgc
      661 cgacggcaag agttccgggg aaatcgccat catcctgagc atctccgaga gcacggtgaa
      721 cttccaccac aagaacatcc agaagaagtt cgacgcgccg aacaagacgc tggctgccgc
      781 ctacgccgcg gcgctgggtc tcatctaata atactagaga ttaaagagga gaaatactag
      841 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      901 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      961 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1021 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1081 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1141 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1201 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1261 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1321 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1381 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1441 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1501 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1561 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1621 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1681 gcctttctgc gtttatatac tagagttgac ggctagctca gtcctaggta cagtgctagc
     1741 tactagagat taaagaggag aaatactaga tgaggaatga cggaggcttt ttgctgtggt
     1801 gggacggttt gcgtagcgag atgcagccga tccacgacag ccagggcgtg ttcgccgtcc
     1861 tggaaaagga agtgcggcgc ctgggcttcg attactacgc ctatggcgtg cgccacacga
     1921 ttcccttcac ccggccgaag accgaggtcc atggcaccta tcccaaggcc tggctggagc
     1981 gataccagat gcagaactac ggggccgtgg atccggcgat cctcaacggc ctgcgctcct
     2041 cggaaatggt ggtctggagc gacagcctgt tcgaccagag ccggatgctc tggaacgagg
     2101 ctcgcgattg gggcctctgt gtcggcgcga ccttgccgat ccgcgcgccg aacaatttgc
     2161 tcagcgtgct ttccgtggcg cgcgaccagc agaacatctc cagcttcgag cgcgaggaaa
     2221 tccgcctgcg gctgcgttgc atgatcgagt tgctgaccca gaagctgacc gacctggagc
     2281 atccgatgct gatgtccaac ccggtctgcc tgagccatcg cgaacgcgag atcctgcaat
     2341 ggaccgccga cggcaagagt tccggggaaa tcgccatcat cctgagcatc tccgagagca
     2401 cggtgaactt ccaccacaag aacatccaga agaagttcga cgcgccgaac aagacgctgg
     2461 ctgccgccta cgccgcggcg ctgggtctca tctaataata ctagagccag gcatcaaata
     2521 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2581 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
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