##gff-version 3
##sequence-region BBa_K1157021 1 2635
BBa_K1157021	.	promoter	1	53	.	+	0	ID=BBa_R0071;Name=BBa_R0071
BBa_K1157021	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation297102;Name=RhlR;Parent=BBa_R0071
BBa_K1157021	.	promoter	19	24	.	+	0	ID=annotation297103;Name=-35;Parent=BBa_R0071
BBa_K1157021	.	promoter	42	47	.	+	0	ID=annotation297104;Name=-10;Parent=BBa_R0071
BBa_K1157021	.	start_codon	53	53	.	+	0	ID=annotation297105;Name=start;Parent=BBa_R0071
BBa_K1157021	.	promoter	1706	1740	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_K1157021	.	ribosome_entry_site	62	76	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	69	73	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157021	.	engineered_region	62	76	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	62	76	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	1749	1763	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	1756	1760	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157021	.	engineered_region	1749	1763	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	1749	1763	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157021	.	CDS	841	1560	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K1157021	.	CDS	841	1560	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1157021	.	ribosome_entry_site	820	834	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	827	831	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1157021	.	engineered_region	820	834	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1157021	.	ribosome_entry_site	820	834	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1157021	.	CDS	1770	2498	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157021	.	terminator	2507	2635	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157021	.	terminator	2507	2586	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157021	.	stem_loop	2518	2561	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157021	.	engineered_region	2507	2586	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157021	.	terminator	2595	2635	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157021	.	stem_loop	2602	2621	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157021	.	stop_codon	2628	2628	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157021	.	engineered_region	2595	2635	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157021	.	polyA_site	2622	2635	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1157021	.	CDS	83	811	.	+	0	ID=BBa_C0171;Name=BBa_C0171
BBa_K1157021	.	terminator	1569	1697	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1157021	.	terminator	1569	1648	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1157021	.	stem_loop	1580	1623	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1157021	.	engineered_region	1569	1648	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1157021	.	terminator	1657	1697	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1157021	.	stem_loop	1664	1683	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1157021	.	stop_codon	1690	1690	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1157021	.	engineered_region	1657	1697	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1157021	.	polyA_site	1684	1697	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1157021
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagattaaagaggagaaatac
tagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagg
gcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcc
cttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggg
gccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagcc
ggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgct
cagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgc
atgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatc
gcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagag
cacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcg
gcgctgggtctcatctaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactgg
agttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgat
gcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcacta
ctttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcc
cgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaa
ggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattgg
aatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattag
acacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctt
ttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttc
ttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacggctagctcagtcctaggtacagtgcta
gctactagagattaaagaggagaaatactagatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagc
gagatgcagccgatccacgacagccagggcgtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattact
acgcctatggcgtgcgccacacgattcccttcacccggccgaagaccgaggtccatggcacctatcccaaggcctggct
ggagcgataccagatgcagaactacggggccgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtc
tggagcgacagcctgttcgaccagagccggatgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgacct
tgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcg
cgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgacccagaagctgaccgacctggagcatccgatgctg
atgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaa
tcgccatcatcctgagcatctccgagagcacggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaa
caagacgctggctgccgcctacgccgcggcgctgggtctcatctaataatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttata