LOCUS       BBa_K1159014            2362 bp    DNA     linear       30-Dec-2025
DEFINITION  Membrane-anchored Erythromycin Esterase Type II in RFC[25] N-Part
ACCESSION   BBa_K1159014
VERSION     BBa_K1159014.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..1360
                     /label=Erythromycin Esterase Type II
     CDS             107..1360
                     /label=BBa_K1159000
     CDS             1..1360
                     /label=BBa_K1159009
     misc_feature    1361..1366
                     /label=25/25 scar
     misc_feature    1361..1366
                     /label=BBa_K1159993
     misc_feature    1373..1396
                     /label=''Strep''-tag II
     misc_feature    1400..1420
                     /label=TEV cleavage site
     misc_feature    1367..1423
                     /label=BBa_K1159309
     misc_feature    1424..1429
                     /label=25/25 scar
     misc_feature    1424..1429
                     /label=BBa_K1159993
     CDS             1430..1615
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    1430..1615
                     /label=BBa_K1159305
     misc_feature    1616..1621
                     /label=25/25 scar
     misc_feature    1616..1621
                     /label=BBa_K1159993
     misc_feature    1622..1645
                     /label=MiddleLinker
     misc_feature    1622..1645
                     /label=BBa_K243005
     misc_feature    1646..1651
                     /label=25/25 scar
     misc_feature    1646..1651
                     /label=BBa_K1159993
     CDS             1652..2362
                     /label=GFPmut1
     CDS             1652..2362
                     /label=BBa_K1159311
     CDS             1430..2362
                     /label=BBa_K1159315
     CDS             1367..2362
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcaggt tcgaagaatg
      121 ggtcaaagat aagcatattc ctttcaaact gaatcaccct gatgataatt acgatgattt
      181 taagccatta agaaaaataa ttggagatac ccgagttgta gcattaggtg aaaattctca
      241 tttcataaaa gagttctttt tgttacgaca tacgcttttg cgttttttta tcgaagatct
      301 aggttttact acgtttgctt ttgaatttgg ttttgctgag ggtcaaatca tcaataactg
      361 gatacatgga caaggaactg acgatgaaat aggcagattc ttaaaacact tctattatcc
      421 agaagagctc aaaaccacat ttctatggct aagggagtac aataaagcag caaaagaaaa
      481 aatcacattt cttggcattg atatacccag aaatggaggt tcatacttac caaatatgga
      541 gatagtgcat gactttttta gaacagcgga taaagaagca ctacacatta tcgatgatgc
      601 atttaatatt gcaaaaaaga ttgattactt ctccacatca caggcagcct taaatttaca
      661 tgagctaaca gattctgaga aatgccgttt aactagccaa ttagctcgag taaaagttcg
      721 ccttgaagct atggccccaa ttcacattga aaaatatggg attgataaat atgagacaat
      781 tctgcattat gccaacggta tgatatactt ggactataac attcaagcta tgtcgggctt
      841 tatttcagga ggcggaatgc agggcgatat gggtgcaaaa gacaaataca tggcagattc
      901 tgtgctgtgg catttaaaaa acccacaaag tgagcagaaa gtgatagtag tagcacataa
      961 tgcacatatt caaaaaacac ccattctgta tgatggattt ctaagttgcc taccaatggg
     1021 ccaaagactt aaaaatgcca ttggtgatga ttatatgtct ttaggtatta cttcttatag
     1081 tgggcataca gcagccctct atccggaagt tgatacaaaa tatggttttc gagttgataa
     1141 cttccaacta caggaaccaa atgaaggttc tgtcgagaaa gctatttctg gttgtggagt
     1201 tactaattct tttgtctttt ttagaaatat tcctgaagat ttacaatcca tcccgaacat
     1261 gattcgattt gattctattt acatgaaagc agaactcgag aaagctttcg atggaatatt
     1321 tcaaattgaa aagtcatctg tatctgaggt cgtttatgaa accggcagcg cttggtctca
     1381 tcctcaattt gaaaagggtg aaaatcttta ttttcaaagc ggaaccggcc ctggacaacc
     1441 tccttttcct cctcctcctc cttttacacc acctcctcca caaactccta acggtgcttc
     1501 tggtgagaac tctactggtg ctattgctgg tggtgttgct gctggtgcag ctcttttgtt
     1561 tgctgctcct gctattggat tcgcttggtg gagaagaaga aggcctattg aggctaccgg
     1621 cggtggttct ggtggtggtt ctggtaccgg cagtaaagga gaagaacttt tcactggagt
     1681 tgtcccaatt cttgttgaat tagatggtga cgttaatggg cacaaatttt ctgtcagtgg
     1741 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     1801 aaaactacct gttccatggc caacacttgt cactactctg acttatggtg ttcaatgctt
     1861 ttcaagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     1921 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
     1981 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     2041 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     2101 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
     2161 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg
     2221 ccctgtcctt tcaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc
     2281 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca
     2341 tggcatggat gagctctaca aa
//