LOCUS       BBa_K1159015            1585 bp    DNA     linear       22-Nov-2024
DEFINITION  Membrane-anchored NanoLuc Luciferase in RFC[25] N-Part
ACCESSION   BBa_K1159015
VERSION     BBa_K1159015.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     CDS             74..583
                     /label=NanoLuc Luciferase
     CDS             74..583
                     /label=BBa_K1159001
     CDS             1..583
                     /label=BBa_K1159006
     misc_feature    584..589
                     /label=25/25 scar
     misc_feature    584..589
                     /label=BBa_K1159993
     misc_feature    596..619
                     /label=''Strep''-tag II
     misc_feature    623..643
                     /label=TEV cleavage site
     misc_feature    590..646
                     /label=BBa_K1159309
     misc_feature    647..652
                     /label=25/25 scar
     misc_feature    647..652
                     /label=BBa_K1159993
     CDS             653..838
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    653..838
                     /label=BBa_K1159305
     misc_feature    839..844
                     /label=25/25 scar
     misc_feature    839..844
                     /label=BBa_K1159993
     misc_feature    845..868
                     /label=MiddleLinker
     misc_feature    845..868
                     /label=BBa_K243005
     misc_feature    869..874
                     /label=25/25 scar
     misc_feature    869..874
                     /label=BBa_K1159993
     CDS             875..1585
                     /label=GFPmut1
     CDS             875..1585
                     /label=BBa_K1159311
     CDS             653..1585
                     /label=BBa_K1159315
     CDS             590..1585
                     /label=BBa_K1159316
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgtgttca ccctcgagga tttcgttgga gattggagac agaccgctgg
      121 atacaacctt gatcaggttt tggagcaggg tggtgtgtct agtcttttcc agaacctcgg
      181 agtgtctgtg acccctatcc agagaatcgt tctctctggt gagaacggac tcaagatcga
      241 tatccacgtg atcatccctt acgagggact ctcaggtgat cagatgggac agatcgagaa
      301 gattttcaag gtggtgtacc ctgttgatga tcaccacttc aaggtgatcc tccactacgg
      361 aactctcgtg atcgatggtg tgaccccaaa catgatcgat tacttcggaa ggccatacga
      421 gggaatcgct gtgttcgatg gaaagaagat taccgttacc ggaaccctct ggaacggaaa
      481 caagattatc gatgagaggc tcatcaaccc tgatggatct ctcttgttca gggtgaccat
      541 caacggtgtg actggttgga gactttgcga gagaatcctt gctaccggca gcgcttggtc
      601 tcatcctcaa tttgaaaagg gtgaaaatct ttattttcaa agcggaaccg gccctggaca
      661 acctcctttt cctcctcctc ctccttttac accacctcct ccacaaactc ctaacggtgc
      721 ttctggtgag aactctactg gtgctattgc tggtggtgtt gctgctggtg cagctctttt
      781 gtttgctgct cctgctattg gattcgcttg gtggagaaga agaaggccta ttgaggctac
      841 cggcggtggt tctggtggtg gttctggtac cggcagtaaa ggagaagaac ttttcactgg
      901 agttgtccca attcttgttg aattagatgg tgacgttaat gggcacaaat tttctgtcag
      961 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1021 tggaaaacta cctgttccat ggccaacact tgtcactact ctgacttatg gtgttcaatg
     1081 cttttcaaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1141 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1201 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1261 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     1321 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     1381 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     1441 tggccctgtc ctttcaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     1501 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     1561 acatggcatg gatgagctct acaaa
//