LOCUS       BBa_K1159016            2632 bp    DNA     linear       22-Nov-2024
DEFINITION  Membrane-anchored Laccase in RFC[25] N-Part
ACCESSION   BBa_K1159016
VERSION     BBa_K1159016.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..1630
                     /label=Laccase from ''Bacillus pumilus''
     CDS             107..1630
                     /label=BBa_K1159002
     CDS             1..1630
                     /label=BBa_K1159011
     misc_feature    1631..1636
                     /label=25/25 scar
     misc_feature    1631..1636
                     /label=BBa_K1159993
     misc_feature    1643..1666
                     /label=''Strep''-tag II
     misc_feature    1670..1690
                     /label=TEV cleavage site
     misc_feature    1637..1693
                     /label=BBa_K1159309
     misc_feature    1694..1699
                     /label=25/25 scar
     misc_feature    1694..1699
                     /label=BBa_K1159993
     CDS             1700..1885
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    1700..1885
                     /label=BBa_K1159305
     misc_feature    1886..1891
                     /label=25/25 scar
     misc_feature    1886..1891
                     /label=BBa_K1159993
     misc_feature    1892..1915
                     /label=MiddleLinker
     misc_feature    1892..1915
                     /label=BBa_K243005
     misc_feature    1916..1921
                     /label=25/25 scar
     misc_feature    1916..1921
                     /label=BBa_K1159993
     CDS             1922..2632
                     /label=GFPmut1
     CDS             1922..2632
                     /label=BBa_K1159311
     CDS             1700..2632
                     /label=BBa_K1159315
     CDS             1637..2632
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcaacc tagaaaaatt
      121 tgttgacgag ctgccaattc cagaagttgc gaagcccgtc aaaaagaacc caaaacaaac
      181 gtattatgaa atcgctatgg aggaggtatt tctaaaagtt catagagatc tgcccccaac
      241 caagctatgg acctataatg gcagtttgcc tggtccaacc attcatgcga atcgaaatga
      301 aaaagtcaaa gtgaaatgga tgaacaaatt gccacttaag cattttctac cagtcgatca
      361 caccattcac gaaggccatc atgatgaacc agaagttaaa accgtcgttc atttacatgg
      421 tggcgtcaca ccagcaagca gtgatggcta tccagaggct tggttttcac gagactttga
      481 agcaaccggc cccttctttg aacgggaggt gtacgaatac ccaaatcatc agcaagcctg
      541 cacattgtgg tatcacgatc atgcgatggc attgacacga ttaaatgtgt atgcgggctt
      601 agctggattt tatttgatct cagatgcgtt tgaaaagtcg ctagaattac cgaagggtga
      661 gtatgatatt ccgctaatga tcatggaccg tacgtttcag gaggatggcg cactgtttta
      721 tccaagcagg ccaaacaaca caccagaaga cagtgacata ccagatccgt ctatcgtgcc
      781 tttcttttgc ggagaaacca ttttggtcaa tggaaaagta tggccgtatt tagaagtaga
      841 gccgcgaaaa tatcgttttc gtattttaaa tgcttccaat acaagaactt acgagctgca
      901 tcttgacaac gatgcgacga ttttgcaaat tggatctgat ggcggctttt taccaagacc
      961 tgttcaccat caatccttta gcattgctcc tgctgaacga tttgatgtca tcatcgattt
     1021 ttcagcttac gaaaacaaaa cgatcaccct taaaaataaa gctggctgcg gacaggaagt
     1081 aaatcctgaa acagatgcaa acatcatgca atttaaagtc actcgaccgc taaaagggag
     1141 agcacctaaa acattacggc ctattttcaa accgcttcca ccacttcggc cttgtcgagc
     1201 tgataaagag cgtacgctca ctcttacggg tacacaggat aaatacggcc gtcctatttt
     1261 attgctagat aaccaatttt ggaatgaccc tgtcacggaa aatcctcgtc ttggcagtgt
     1321 ggaggtttgg tctatcgtca atccaacaag gggcacacat cctattcatt tacaccttgt
     1381 tcaattcaga gtgatagaca gaagaccatt tgatactgag gtctatcaat cgacagggga
     1441 cattgtgtat acaggaccaa acgaagcacc tcccttacat gaacaaggct acaaggacac
     1501 cattcaagcg catgccggtg aagtcattcg gatcatcgct cgctttgttc catacagcgg
     1561 caggtatgtg tggcattgtc atatattaga gcacgaggat tatgacatga tgcggccgat
     1621 ggatatcatc accggcagcg cttggtctca tcctcaattt gaaaagggtg aaaatcttta
     1681 ttttcaaagc ggaaccggcc ctggacaacc tccttttcct cctcctcctc cttttacacc
     1741 acctcctcca caaactccta acggtgcttc tggtgagaac tctactggtg ctattgctgg
     1801 tggtgttgct gctggtgcag ctcttttgtt tgctgctcct gctattggat tcgcttggtg
     1861 gagaagaaga aggcctattg aggctaccgg cggtggttct ggtggtggtt ctggtaccgg
     1921 cagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1981 cgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     2041 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     2101 cactactctg acttatggtg ttcaatgctt ttcaagatac ccagatcata tgaaacagca
     2161 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     2221 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     2281 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     2341 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     2401 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     2461 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt tcaccagaca accattacct
     2521 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     2581 tgagtttgta acagctgctg ggattacaca tggcatggat gagctctaca aa
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