Membrane-anchored Laccase in RFC[25] N-Part
Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
Secretory Laccase (SERK-SigP_Laccase) in RFC[25] N-Part
SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]
This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration.
Laccase from Bacillus pumilus in RFC[25]
Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
19 AA Linker with N-terminal Strep-tag II and C-terminal TEV cleavage site in RFC[25]
This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]
This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
Transmembrane Domain of SERK Receptor from ''P. patens''
This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix.
Oligos synthesized by sigma. Hybridized by PCR.
This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
Conversion of the iGEM parts registry to SBOL2.1