##gff-version 3
##sequence-region BBa_K1159017 1 1630
BBa_K1159017	.	sequence_feature	101	106	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993
BBa_K1159017	.	sequence_feature	101	106	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159017	.	CDS	635	1630	.	+	0	ID=BBa_K1159316;Name=BBa_K1159316
BBa_K1159017	.	sequence_feature	692	697	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159316
BBa_K1159017	.	sequence_feature	692	697	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159017	.	polypeptide_domain	635	691	.	+	0	ID=BBa_K1159309;Name=BBa_K1159309;Parent=BBa_K1159316
BBa_K1159017	.	sequence_feature	668	688	.	+	0	ID=annotation2342591;Name=TEV cleavage site;Parent=BBa_K1159309
BBa_K1159017	.	tag	641	664	.	+	0	ID=annotation2342578;Name=''Strep''-tag II;Parent=BBa_K1159309
BBa_K1159017	.	CDS	698	1630	.	+	0	ID=BBa_K1159315;Name=BBa_K1159315;Parent=BBa_K1159316
BBa_K1159017	.	sequence_feature	890	913	.	+	0	ID=BBa_K243005;Name=BBa_K243005;Parent=BBa_K1159315
BBa_K1159017	.	engineered_region	890	913	.	+	0	ID=annotation2041884;Name=MiddleLinker;Parent=BBa_K243005
BBa_K1159017	.	polypeptide_domain	698	883	.	+	0	ID=BBa_K1159305;Name=BBa_K1159305;Parent=BBa_K1159315
BBa_K1159017	.	CDS	698	883	.	+	0	ID=annotation2343588;Name=Transmembrane Domain of SERK Receptor from ''P. patens'';Parent=BBa_K1159305
BBa_K1159017	.	sequence_feature	884	889	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159017	.	sequence_feature	884	889	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159017	.	sequence_feature	914	919	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159315
BBa_K1159017	.	sequence_feature	914	919	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159017	.	CDS	920	1630	.	+	0	ID=BBa_K1159311;Name=BBa_K1159311;Parent=BBa_K1159315
BBa_K1159017	.	CDS	920	1630	.	+	0	ID=annotation2343590;Name=GFPmut1;Parent=BBa_K1159311
BBa_K1159017	.	sequence_feature	629	634	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993
BBa_K1159017	.	sequence_feature	629	634	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159017	.	polypeptide_domain	1	100	.	+	0	ID=BBa_K1159303;Name=BBa_K1159303
BBa_K1159017	.	CDS	5	100	.	+	0	ID=annotation2341513;Name=SERK Signal Peptide;Parent=BBa_K1159303
BBa_K1159017	.	ribosome_entry_site	1	4	.	+	0	ID=annotation2341512;Name=Consensus Sequence for ''P. patens'';Parent=BBa_K1159303
BBa_K1159017	.	CDS	107	628	.	+	0	ID=BBa_K1159003;Name=BBa_K1159003
BBa_K1159017	.	sequence_alteration	446	448	.	+	0	ID=annotation2343158;Name=Ser --> Thr;Parent=BBa_K1159003
BBa_K1159017	.	sequence_alteration	239	241	.	+	0	ID=annotation2343143;Name=Ala --> Ile;Parent=BBa_K1159003
BBa_K1159017	.	CDS	107	628	.	+	0	ID=annotation2343011;Name=FluA triple mutant;Parent=BBa_K1159003
BBa_K1159017	.	sequence_alteration	389	391	.	+	0	ID=annotation2343157;Name=Arg --> Lys;Parent=BBa_K1159003
>BBa_K1159017
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgct
gagggtgatgctcttaacaccaccggcgacgtgtaccacgacggtgcctgtcccgaagtcaagccagtcgacaacttcg
actggtcccagtaccatggtaaatggtgggaagtcgccaaataccccagtcccaacgggaagtacggaaagtgcggatg
gatcgagtacactcctgaaggcaagagtgtcaaagtttcgcgctacgatgtaatccacggcaaggaatactttatggaa
ggtaccgcctacccagttggtgactccaagattggaaagatctaccacagcaggactgttggaggttacaccaaaaaga
cggtattcaacgtactctccactgacaacaagaactacatcatcggatacacgtgccgctacgacgaggacaagaaggg
acactgggaccatgtctgggtgctctccagaagcatggtccttactggtgaagccaagaccgctgtcgagaactacctt
atcggctccccagtagtcgactcccagaaactggtatacagtgacttctctgaagccgcctgcaaggtcaacaataccg
gcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctcc
ttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgct
attgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaa
ggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagt
tgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgca
acatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactc
tgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccga
aggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggt
gatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaat
acaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagaca
caacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttca
ccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttg
agtttgtaacagctgctgggattacacatggcatggatgagctctacaaa