BBa_K1159017
type: ComponentDefinition
persistent identity: BBa K1159017
display id: BBa_K1159017
version: 1
was generated by: iGEM to SBOL conversion
description: Membrane-anchored FluA triple mutant in RFC[25] N-Part
created: 2013-05-16T11:00:00Z
modified: 2015-05-08T01:09:30Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159303
component: BBa_K1159316
component: BBa_K1159993
component: BBa_K1159003
sequence annotation: BBa_K1159303
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159003
sequence annotation: BBa_K1159316
sequence: https://synbiohub.org/public/igem/BBa_K1159017_sequence/1

BBa_K1159303
type: Component
persistent identity: component2346544
display id: component2346544
version: 1
top level: BBa_K1159017
definition: BBa_K1159303
access: public

BBa_K1159316
type: Component
persistent identity: component2346570
display id: component2346570
version: 1
top level: BBa_K1159017
definition: BBa_K1159316
access: public

BBa_K1159993
type: Component
persistent identity: component2346553
display id: component2346553
version: 1
top level: BBa_K1159017
definition: BBa_K1159993
access: public

BBa_K1159003
type: Component
persistent identity: component2346551
display id: component2346551
version: 1
top level: BBa_K1159017
definition: BBa_K1159003
access: public

BBa_K1159993
type: Component
persistent identity: component2346546
display id: component2346546
version: 1
top level: BBa_K1159017
definition: BBa_K1159993
access: public

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2346544
display id: annotation2346544
version: 1
top level: BBa_K1159017
location: https://synbiohub.org/public/igem/BBa_K1159017/annotation2346544/range2346544/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159017/annotation2346544/range2346544/1
type: Range
persistent identity: range2346544
display id: range2346544
version: 1
top level: BBa_K1159017
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346546
display id: annotation2346546
version: 1
top level: BBa_K1159017
location: https://synbiohub.org/public/igem/BBa_K1159017/annotation2346546/range2346546/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159017/annotation2346546/range2346546/1
type: Range
persistent identity: range2346546
display id: range2346546
version: 1
top level: BBa_K1159017
direction: direction/reverse
start: 101
end: 106
orientation: inline

BBa_K1159003
type: SequenceAnnotation
persistent identity: annotation2346551
display id: annotation2346551
version: 1
top level: BBa_K1159017
location: https://synbiohub.org/public/igem/BBa_K1159017/annotation2346551/range2346551/1
component: BBa_K1159003

https://synbiohub.org/public/igem/BBa_K1159017/annotation2346551/range2346551/1
type: Range
persistent identity: range2346551
display id: range2346551
version: 1
top level: BBa_K1159017
direction: direction/reverse
start: 107
end: 628
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346553
display id: annotation2346553
version: 1
top level: BBa_K1159017
location: https://synbiohub.org/public/igem/BBa_K1159017/annotation2346553/range2346553/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159017/annotation2346553/range2346553/1
type: Range
persistent identity: range2346553
display id: range2346553
version: 1
top level: BBa_K1159017
direction: direction/reverse
start: 629
end: 634
orientation: inline

BBa_K1159316
type: SequenceAnnotation
persistent identity: annotation2346570
display id: annotation2346570
version: 1
top level: BBa_K1159017
location: https://synbiohub.org/public/igem/BBa_K1159017/annotation2346570/range2346570/1
component: BBa_K1159316

https://synbiohub.org/public/igem/BBa_K1159017/annotation2346570/range2346570/1
type: Range
persistent identity: range2346570
display id: range2346570
version: 1
top level: BBa_K1159017
direction: direction/reverse
start: 635
end: 1630
orientation: inline

BBa_K1159003
type: ComponentDefinition
persistent identity: BBa K1159003
display id: BBa_K1159003
version: 1
was generated by: iGEM to SBOL conversion
description: Engineered Fluorescein-Binding Anticalin FluA (triple mutant variant) in RFC[25]
created: 2013-05-16T11:00:00Z
modified: 2015-05-28T03:07:12Z
mutable provenance: x
owned by: james
owned by: myers
mutable description: x
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 14732
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FluA triple mutant
sequence annotation: Ala --> Ile
sequence annotation: Arg --> Lys
sequence annotation: Ser --> Thr
sequence: https://synbiohub.org/public/igem/BBa_K1159003_sequence/1

FluA triple mutant
type: SequenceAnnotation
persistent identity: annotation2343011
display id: annotation2343011
version: 1
top level: BBa_K1159003
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343011/range2343011/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343011/range2343011/1
type: Range
persistent identity: range2343011
display id: range2343011
version: 1
top level: BBa_K1159003
direction: direction/forward
start: 1
end: 522
orientation: inline

Ala --> Ile
type: SequenceAnnotation
persistent identity: annotation2343143
display id: annotation2343143
version: 1
top level: BBa_K1159003
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343143/range2343143/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343143/range2343143/1
type: Range
persistent identity: range2343143
display id: range2343143
version: 1
top level: BBa_K1159003
direction: direction/forward
start: 133
end: 135
orientation: inline

Arg --> Lys
type: SequenceAnnotation
persistent identity: annotation2343157
display id: annotation2343157
version: 1
top level: BBa_K1159003
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343157/range2343157/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343157/range2343157/1
type: Range
persistent identity: range2343157
display id: range2343157
version: 1
top level: BBa_K1159003
direction: direction/forward
start: 283
end: 285
orientation: inline

Ser --> Thr
type: SequenceAnnotation
persistent identity: annotation2343158
display id: annotation2343158
version: 1
top level: BBa_K1159003
location: https://synbiohub.org/public/igem/BBa_K1159003/annotation2343158/range2343158/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K1159003/annotation2343158/range2343158/1
type: Range
persistent identity: range2343158
display id: range2343158
version: 1
top level: BBa_K1159003
direction: direction/forward
start: 340
end: 342
orientation: inline

BBa_K1159303
type: ComponentDefinition
persistent identity: BBa K1159303
display id: BBa_K1159303
version: 1
was generated by: iGEM to SBOL conversion
description: Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:49Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Consensus Sequence for ''P. patens''
sequence annotation: SERK Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159303_sequence/1

Consensus Sequence for ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2341512
display id: annotation2341512
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
type: Range
persistent identity: range2341512
display id: range2341512
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 1
end: 4
orientation: inline

SERK Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341513
display id: annotation2341513
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
type: Range
persistent identity: range2341513
display id: range2341513
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 5
end: 100
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159316
type: ComponentDefinition
persistent identity: BBa K1159316
display id: BBa_K1159316
version: 1
was generated by: iGEM to SBOL conversion
description: SERK-Receptor TMD with C-terminal GFP and N-terminal Strep-tag II TEV-site linker in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:10Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159315
component: BBa_K1159993
component: BBa_K1159309
sequence annotation: BBa_K1159309
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159315
sequence: https://synbiohub.org/public/igem/BBa_K1159316_sequence/1

BBa_K1159315
type: Component
persistent identity: component2346363
display id: component2346363
version: 1
top level: BBa_K1159316
definition: BBa_K1159315
access: public

BBa_K1159993
type: Component
persistent identity: component2346352
display id: component2346352
version: 1
top level: BBa_K1159316
definition: BBa_K1159993
access: public

BBa_K1159309
type: Component
persistent identity: component2346350
display id: component2346350
version: 1
top level: BBa_K1159316
definition: BBa_K1159309
access: public

BBa_K1159309
type: SequenceAnnotation
persistent identity: annotation2346350
display id: annotation2346350
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
component: BBa_K1159309

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346350/range2346350/1
type: Range
persistent identity: range2346350
display id: range2346350
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346352
display id: annotation2346352
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346352/range2346352/1
type: Range
persistent identity: range2346352
display id: range2346352
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 58
end: 63
orientation: inline

BBa_K1159315
type: SequenceAnnotation
persistent identity: annotation2346363
display id: annotation2346363
version: 1
top level: BBa_K1159316
location: https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
component: BBa_K1159315

https://synbiohub.org/public/igem/BBa_K1159316/annotation2346363/range2346363/1
type: Range
persistent identity: range2346363
display id: range2346363
version: 1
top level: BBa_K1159316
direction: direction/reverse
start: 64
end: 996
orientation: inline

BBa_K1159309
type: ComponentDefinition
persistent identity: BBa K1159309
display id: BBa_K1159309
version: 1
was generated by: iGEM to SBOL conversion
description: 19 AA Linker with N-terminal Strep-tag II and C-terminal TEV cleavage site in RFC[25]
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: ''Strep''-tag II
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1159309_sequence/1

''Strep''-tag II
type: SequenceAnnotation
persistent identity: annotation2342578
display id: annotation2342578
version: 1
top level: BBa_K1159309
location: https://synbiohub.org/public/igem/BBa_K1159309/annotation2342578/range2342578/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1159309/annotation2342578/range2342578/1
type: Range
persistent identity: range2342578
display id: range2342578
version: 1
top level: BBa_K1159309
direction: direction/forward
start: 7
end: 30
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2342591
display id: annotation2342591
version: 1
top level: BBa_K1159309
location: https://synbiohub.org/public/igem/BBa_K1159309/annotation2342591/range2342591/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159309/annotation2342591/range2342591/1
type: Range
persistent identity: range2342591
display id: range2342591
version: 1
top level: BBa_K1159309
direction: direction/forward
start: 34
end: 54
orientation: inline

BBa_K1159315
type: ComponentDefinition
persistent identity: BBa K1159315
display id: BBa_K1159315
version: 1
was generated by: iGEM to SBOL conversion
description: TMD of SERK Receptor from Physcomitrella patens with C-terminal GFP fusion in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-28T03:06:34Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain of SERK Receptor from Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159993
component: BBa_K243005
component: BBa_K1159305
component: BBa_K1159311
sequence annotation: BBa_K1159305
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243005
sequence annotation: BBa_K1159311
sequence: https://synbiohub.org/public/igem/BBa_K1159315_sequence/1

BBa_K1159993
type: Component
persistent identity: component2346340
display id: component2346340
version: 1
top level: BBa_K1159315
definition: BBa_K1159993
access: public

BBa_K243005
type: Component
persistent identity: component2346338
display id: component2346338
version: 1
top level: BBa_K1159315
definition: MiddleLink
access: public

BBa_K1159993
type: Component
persistent identity: component2346336
display id: component2346336
version: 1
top level: BBa_K1159315
definition: BBa_K1159993
access: public

BBa_K1159305
type: Component
persistent identity: component2346334
display id: component2346334
version: 1
top level: BBa_K1159315
definition: BBa_K1159305
access: public

BBa_K1159311
type: Component
persistent identity: component2346342
display id: component2346342
version: 1
top level: BBa_K1159315
definition: BBa_K1159311
access: public

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2346334
display id: annotation2346334
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
component: BBa_K1159305

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346334/range2346334/1
type: Range
persistent identity: range2346334
display id: range2346334
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 1
end: 186
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346336
display id: annotation2346336
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346336/range2346336/1
type: Range
persistent identity: range2346336
display id: range2346336
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 187
end: 192
orientation: inline

BBa_K243005
type: SequenceAnnotation
persistent identity: annotation2346338
display id: annotation2346338
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
component: BBa_K243005

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346338/range2346338/1
type: Range
persistent identity: range2346338
display id: range2346338
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 193
end: 216
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346340
display id: annotation2346340
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346340/range2346340/1
type: Range
persistent identity: range2346340
display id: range2346340
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 217
end: 222
orientation: inline

BBa_K1159311
type: SequenceAnnotation
persistent identity: annotation2346342
display id: annotation2346342
version: 1
top level: BBa_K1159315
location: https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
component: BBa_K1159311

https://synbiohub.org/public/igem/BBa_K1159315/annotation2346342/range2346342/1
type: Range
persistent identity: range2346342
display id: range2346342
version: 1
top level: BBa_K1159315
direction: direction/reverse
start: 223
end: 933
orientation: inline

BBa_K1159305
type: ComponentDefinition
persistent identity: BBa K1159305
display id: BBa_K1159305
version: 1
was generated by: iGEM to SBOL conversion
description: Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:11Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Transmembrane Domain of SERK Receptor from ''P. patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159305_sequence/1

Transmembrane Domain of SERK Receptor from ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2343588
display id: annotation2343588
version: 1
top level: BBa_K1159305
location: https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
type: Range
persistent identity: range2343588
display id: range2343588
version: 1
top level: BBa_K1159305
direction: direction/forward
start: 1
end: 186
orientation: inline

BBa_K1159311
type: ComponentDefinition
persistent identity: BBa K1159311
display id: BBa_K1159311
version: 1
was generated by: iGEM to SBOL conversion
description: GFPmut1
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Aequarea victoria''
owned by: james
owned by: myers
mutable description: This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFPmut1
sequence: https://synbiohub.org/public/igem/BBa_K1159311_sequence/1

GFPmut1
type: SequenceAnnotation
persistent identity: annotation2343590
display id: annotation2343590
version: 1
top level: BBa_K1159311
location: https://synbiohub.org/public/igem/BBa_K1159311/annotation2343590/range2343590/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159311/annotation2343590/range2343590/1
type: Range
persistent identity: range2343590
display id: range2343590
version: 1
top level: BBa_K1159311
direction: direction/forward
start: 1
end: 711
orientation: inline

MiddleLink
type: ComponentDefinition
persistent identity: BBa K243005
display id: BBa_K243005
version: 1
was generated by: iGEM to SBOL conversion
description: Middle Linker ( Gly-Gly-Ser-Gly)x2
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Oligos synthesized by sigma. Hybridized by PCR.
owned by: james
owned by: myers
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly .
discontinued: false
dominant: false
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence annotation: MiddleLinker
sequence: https://synbiohub.org/public/igem/BBa_K243005_sequence/1

MiddleLinker
type: SequenceAnnotation
persistent identity: annotation2041884
display id: annotation2041884
version: 1
top level: MiddleLink
location: https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K243005/annotation2041884/range2041884/1
type: Range
persistent identity: range2041884
display id: range2041884
version: 1
top level: MiddleLink
direction: direction/forward
start: 1
end: 24
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159017_sequence/1
type: Sequence
persistent identity: BBa K1159017 sequence
display id: BBa_K1159017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgacgtgtaccacgacggtgcctgtcccgaagtcaagccagtcgacaacttcgactggtcccagtaccatggtaaatggtgggaagtcgccaaataccccagtcccaacgggaagtacggaaagtgcggatggatcgagtacactcctgaaggcaagagtgtcaaagtttcgcgctacgatgtaatccacggcaaggaatactttatggaaggtaccgcctacccagttggtgactccaagattggaaagatctaccacagcaggactgttggaggttacaccaaaaagacggtattcaacgtactctccactgacaacaagaactacatcatcggatacacgtgccgctacgacgaggacaagaagggacactgggaccatgtctgggtgctctccagaagcatggtccttactggtgaagccaagaccgctgtcgagaactaccttatcggctccccagtagtcgactcccagaaactggtatacagtgacttctctgaagccgcctgcaaggtcaacaataccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159003_sequence/1
type: Sequence
persistent identity: BBa K1159003 sequence
display id: BBa_K1159003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gacgtgtaccacgacggtgcctgtcccgaagtcaagccagtcgacaacttcgactggtcccagtaccatggtaaatggtgggaagtcgccaaataccccagtcccaacgggaagtacggaaagtgcggatggatcgagtacactcctgaaggcaagagtgtcaaagtttcgcgctacgatgtaatccacggcaaggaatactttatggaaggtaccgcctacccagttggtgactccaagattggaaagatctaccacagcaggactgttggaggttacaccaaaaagacggtattcaacgtactctccactgacaacaagaactacatcatcggatacacgtgccgctacgacgaggacaagaagggacactgggaccatgtctgggtgctctccagaagcatggtccttactggtgaagccaagaccgctgtcgagaactaccttatcggctccccagtagtcgactcccagaaactggtatacagtgacttctctgaagccgcctgcaaggtcaacaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159303_sequence/1
type: Sequence
persistent identity: BBa K1159303 sequence
display id: BBa_K1159303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159316_sequence/1
type: Sequence
persistent identity: BBa K1159316 sequence
display id: BBa_K1159316_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159309_sequence/1
type: Sequence
persistent identity: BBa K1159309 sequence
display id: BBa_K1159309_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159315_sequence/1
type: Sequence
persistent identity: BBa K1159315 sequence
display id: BBa_K1159315_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159305_sequence/1
type: Sequence
persistent identity: BBa K1159305 sequence
display id: BBa_K1159305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159311_sequence/1
type: Sequence
persistent identity: BBa K1159311 sequence
display id: BBa_K1159311_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243005_sequence/1
type: Sequence
persistent identity: BBa K243005 sequence
display id: BBa_K243005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctggtggtggttctggt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z