LOCUS       BBa_K1159019            2080 bp    DNA     linear       22-Nov-2024
DEFINITION  Membrane-anchored Human Protein Phosphatase 1 (PP1) in RFC[25] 
            N-Part
ACCESSION   BBa_K1159019
VERSION     BBa_K1159019.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..1078
                     /label=Human Protein Phosphatase 1
     CDS             107..1078
                     /label=BBa_K1159004
     CDS             1..1078
                     /label=BBa_K1159013
     misc_feature    1079..1084
                     /label=25/25 scar
     misc_feature    1079..1084
                     /label=BBa_K1159993
     misc_feature    1091..1114
                     /label=''Strep''-tag II
     misc_feature    1118..1138
                     /label=TEV cleavage site
     misc_feature    1085..1141
                     /label=BBa_K1159309
     misc_feature    1142..1147
                     /label=25/25 scar
     misc_feature    1142..1147
                     /label=BBa_K1159993
     CDS             1148..1333
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    1148..1333
                     /label=BBa_K1159305
     misc_feature    1334..1339
                     /label=25/25 scar
     misc_feature    1334..1339
                     /label=BBa_K1159993
     misc_feature    1340..1363
                     /label=MiddleLinker
     misc_feature    1340..1363
                     /label=BBa_K243005
     misc_feature    1364..1369
                     /label=25/25 scar
     misc_feature    1364..1369
                     /label=BBa_K1159993
     CDS             1370..2080
                     /label=GFPmut1
     CDS             1370..2080
                     /label=BBa_K1159311
     CDS             1148..2080
                     /label=BBa_K1159315
     CDS             1085..2080
                     /label=BBa_K1159316
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcggat ccgcggattt
      121 agataaactc aacatcgaca gcattatcca acggctgctg gaagtgagag ggtccaagcc
      181 tggtaagaat gtccagcttc aggagaatga aatcagagga ctgtgcttaa agtctcgtga
      241 aatctttctc agtcagccta tcctactaga acttgaagca ccactcaaaa tatgtggtga
      301 catccatgga caatactatg atttgctgcg actttttgag tacggtggtt tcccaccaga
      361 aagcaactac ctgtttcttg gggactatgt ggacagggga aagcagtcat tggagacgat
      421 ctgcctctta ctggcctaca aaataaaata tcctgagaat ttttttcttc tcagagggaa
      481 ccatgaatgt gccagcatca acagaattta tggattttat gatgaatgta aaagaagata
      541 caacattaaa ctatggaaaa ctttcacaga ctgttttaac tgtttaccga tagcagccat
      601 cgtggatgag aagatattct gctgtcatgg aggtttatca ccagatcttc aatctatgga
      661 gcagattcgg cgaattatgc gaccaactga tgtaccagat caaggtcttc tttgtgatct
      721 tttgtggtct gaccccgata aagatgtctt aggctggggt gaaaatgaca gaggagtgtc
      781 cttcacattt ggtgcagaag tggttgcaaa atttctccat aagcatgatt tggatcttat
      841 atgtagagcc catcaggtgg ttgaagatgg atatgaattt tttgcaaaga ggcagttggt
      901 cactctgttt tctgcgccca attattgcgg agagtttgac aatgcaggtg ccatgatgag
      961 tgtggatgaa acactaatgt gttcttttca gattttaaag cctgccgaga aaaagaagcc
     1021 aaatgccacg agacctgtaa cgcctccaag gggtatgatc acaaagcaag caaagaaaac
     1081 cggcagcgct tggtctcatc ctcaatttga aaagggtgaa aatctttatt ttcaaagcgg
     1141 aaccggccct ggacaacctc cttttcctcc tcctcctcct tttacaccac ctcctccaca
     1201 aactcctaac ggtgcttctg gtgagaactc tactggtgct attgctggtg gtgttgctgc
     1261 tggtgcagct cttttgtttg ctgctcctgc tattggattc gcttggtgga gaagaagaag
     1321 gcctattgag gctaccggcg gtggttctgg tggtggttct ggtaccggca gtaaaggaga
     1381 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgacg ttaatgggca
     1441 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
     1501 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactctgac
     1561 ttatggtgtt caatgctttt caagataccc agatcatatg aaacagcatg actttttcaa
     1621 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
     1681 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
     1741 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
     1801 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
     1861 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
     1921 tactccaatt ggcgatggcc ctgtcctttc accagacaac cattacctgt ccacacaatc
     1981 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     2041 agctgctggg attacacatg gcatggatga gctctacaaa
//