BBa_K1159316 1 BBa_K1159316 SERK-Receptor TMD with C-terminal GFP and N-terminal <i>Strep</i>-tag II TEV-site linker in RFC[25] 2013-09-15T11:00:00Z 2015-06-11T01:53:10Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346352 1 BBa_K1159993 component2346363 1 BBa_K1159315 component2346350 1 BBa_K1159309 annotation2346363 1 BBa_K1159315 range2346363 1 64 996 annotation2346352 1 BBa_K1159993 range2346352 1 58 63 annotation2346350 1 BBa_K1159309 range2346350 1 1 57 BBa_K1159303 1 BBa_K1159303 Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:49Z ''Physcomitrella patens'' This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2341513 1 SERK Signal Peptide range2341513 1 5 100 annotation2341512 1 Consensus Sequence for ''P. patens'' range2341512 1 1 4 BBa_K1159993 1 BBa_K1159993 25/25 Assembly Scar 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2342863 1 25/25 scar range2342863 1 1 6 BBa_K1159019 1 BBa_K1159019 Membrane-anchored Human Protein Phosphatase 1 (PP1) in RFC[25] N-Part 2013-05-16T11:00:00Z 2015-05-08T01:09:31Z x x false false _1471_ 0 14732 9 In stock false x false TU Munich 2013 component2346662 1 BBa_K1159993 component2346679 1 BBa_K1159316 component2346660 1 BBa_K1159013 annotation2346660 1 BBa_K1159013 range2346660 1 1 1078 annotation2346662 1 BBa_K1159993 range2346662 1 1079 1084 annotation2346679 1 BBa_K1159316 range2346679 1 1085 2080 BBa_K1159013 1 BBa_K1159013 Secretory Human Protein Phosphatase 1 (SERK-SigP_PP1) in RFC[25] N-Part 2013-05-16T11:00:00Z 2015-05-08T01:09:30Z x x false false _1471_ 0 14732 9 In stock false x false TU Munich 2013 component2343702 1 BBa_K1159004 component2343698 1 BBa_K1159303 component2343700 1 BBa_K1159993 annotation2343700 1 BBa_K1159993 range2343700 1 101 106 annotation2343702 1 BBa_K1159004 range2343702 1 107 1078 annotation2343698 1 BBa_K1159303 range2343698 1 1 100 BBa_K1159309 1 BBa_K1159309 19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25] 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix. false false _1471_ 0 11507 9 In stock false x false TU Munich 2013 annotation2342578 1 ''Strep''-tag II range2342578 1 7 30 annotation2342591 1 TEV cleavage site range2342591 1 34 54 BBa_K1159311 1 BBa_K1159311 GFPmut1 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z ''Aequarea victoria'' This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2343590 1 GFPmut1 range2343590 1 1 711 BBa_K243005 1 MiddleLink Middle Linker ( Gly-Gly-Ser-Gly)x2 2009-10-13T11:00:00Z 2015-05-08T01:11:37Z Oligos synthesized by sigma. Hybridized by PCR. This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly . false false _352_ 0 4732 9 It's complicated false None. false Freiburg Bioware09 annotation2041884 1 MiddleLinker range2041884 1 1 24 BBa_K1159004 1 BBa_K1159004 Human protein phosphatase 1 (PP1) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:30Z ''Homo sapiens'' Human protein phosphatase 1 binds the toxic oligopeptide produces by gree-blue algae. This part is flanked by RFC[25] pre- and suffix. false false _1471_ 0 11507 9 In stock false x false TU Munich 2013 annotation2343333 1 Human Protein Phosphatase 1 range2343333 1 1 972 BBa_K1159305 1 BBa_K1159305 Transmembrane domain of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:11Z ''Physcomitrella patens'' This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2343588 1 Transmembrane Domain of SERK Receptor from ''P. patens'' range2343588 1 1 186 BBa_K1159315 1 BBa_K1159315 TMD of SERK Receptor from <i>Physcomitrella patens</i> with C-terminal GFP fusion in RFC[25] 2013-09-15T11:00:00Z 2015-05-28T03:06:34Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from <i>Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346336 1 BBa_K1159993 component2346342 1 BBa_K1159311 component2346334 1 BBa_K1159305 component2346338 1 BBa_K243005 component2346340 1 BBa_K1159993 annotation2346340 1 BBa_K1159993 range2346340 1 217 222 annotation2346338 1 BBa_K243005 range2346338 1 193 216 annotation2346334 1 BBa_K1159305 range2346334 1 1 186 annotation2346336 1 BBa_K1159993 range2346336 1 187 192 annotation2346342 1 BBa_K1159311 range2346342 1 223 933 BBa_K1159993_sequence 1 accggc BBa_K1159315_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159013_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcggatccgcggatttagataaactcaacatcgacagcattatccaacggctgctggaagtgagagggtccaagcctggtaagaatgtccagcttcaggagaatgaaatcagaggactgtgcttaaagtctcgtgaaatctttctcagtcagcctatcctactagaacttgaagcaccactcaaaatatgtggtgacatccatggacaatactatgatttgctgcgactttttgagtacggtggtttcccaccagaaagcaactacctgtttcttggggactatgtggacaggggaaagcagtcattggagacgatctgcctcttactggcctacaaaataaaatatcctgagaatttttttcttctcagagggaaccatgaatgtgccagcatcaacagaatttatggattttatgatgaatgtaaaagaagatacaacattaaactatggaaaactttcacagactgttttaactgtttaccgatagcagccatcgtggatgagaagatattctgctgtcatggaggtttatcaccagatcttcaatctatggagcagattcggcgaattatgcgaccaactgatgtaccagatcaaggtcttctttgtgatcttttgtggtctgaccccgataaagatgtcttaggctggggtgaaaatgacagaggagtgtccttcacatttggtgcagaagtggttgcaaaatttctccataagcatgatttggatcttatatgtagagcccatcaggtggttgaagatggatatgaattttttgcaaagaggcagttggtcactctgttttctgcgcccaattattgcggagagtttgacaatgcaggtgccatgatgagtgtggatgaaacactaatgtgttcttttcagattttaaagcctgccgagaaaaagaagccaaatgccacgagacctgtaacgcctccaaggggtatgatcacaaagcaagcaaagaaa BBa_K1159303_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc BBa_K243005_sequence 1 ggtggttctggtggtggttctggt BBa_K1159309_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcgga BBa_K1159019_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcggatccgcggatttagataaactcaacatcgacagcattatccaacggctgctggaagtgagagggtccaagcctggtaagaatgtccagcttcaggagaatgaaatcagaggactgtgcttaaagtctcgtgaaatctttctcagtcagcctatcctactagaacttgaagcaccactcaaaatatgtggtgacatccatggacaatactatgatttgctgcgactttttgagtacggtggtttcccaccagaaagcaactacctgtttcttggggactatgtggacaggggaaagcagtcattggagacgatctgcctcttactggcctacaaaataaaatatcctgagaatttttttcttctcagagggaaccatgaatgtgccagcatcaacagaatttatggattttatgatgaatgtaaaagaagatacaacattaaactatggaaaactttcacagactgttttaactgtttaccgatagcagccatcgtggatgagaagatattctgctgtcatggaggtttatcaccagatcttcaatctatggagcagattcggcgaattatgcgaccaactgatgtaccagatcaaggtcttctttgtgatcttttgtggtctgaccccgataaagatgtcttaggctggggtgaaaatgacagaggagtgtccttcacatttggtgcagaagtggttgcaaaatttctccataagcatgatttggatcttatatgtagagcccatcaggtggttgaagatggatatgaattttttgcaaagaggcagttggtcactctgttttctgcgcccaattattgcggagagtttgacaatgcaggtgccatgatgagtgtggatgaaacactaatgtgttcttttcagattttaaagcctgccgagaaaaagaagccaaatgccacgagacctgtaacgcctccaaggggtatgatcacaaagcaagcaaagaaaaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159311_sequence 1 agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159305_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct BBa_K1159316_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159004_sequence 1 ggatccgcggatttagataaactcaacatcgacagcattatccaacggctgctggaagtgagagggtccaagcctggtaagaatgtccagcttcaggagaatgaaatcagaggactgtgcttaaagtctcgtgaaatctttctcagtcagcctatcctactagaacttgaagcaccactcaaaatatgtggtgacatccatggacaatactatgatttgctgcgactttttgagtacggtggtttcccaccagaaagcaactacctgtttcttggggactatgtggacaggggaaagcagtcattggagacgatctgcctcttactggcctacaaaataaaatatcctgagaatttttttcttctcagagggaaccatgaatgtgccagcatcaacagaatttatggattttatgatgaatgtaaaagaagatacaacattaaactatggaaaactttcacagactgttttaactgtttaccgatagcagccatcgtggatgagaagatattctgctgtcatggaggtttatcaccagatcttcaatctatggagcagattcggcgaattatgcgaccaactgatgtaccagatcaaggtcttctttgtgatcttttgtggtctgaccccgataaagatgtcttaggctggggtgaaaatgacagaggagtgtccttcacatttggtgcagaagtggttgcaaaatttctccataagcatgatttggatcttatatgtagagcccatcaggtggttgaagatggatatgaattttttgcaaagaggcagttggtcactctgttttctgcgcccaattattgcggagagtttgacaatgcaggtgccatgatgagtgtggatgaaacactaatgtgttcttttcagattttaaagcctgccgagaaaaagaagccaaatgccacgagacctgtaacgcctccaaggggtatgatcacaaagcaagcaaagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z