LOCUS BBa_K1159102 354 bp DNA linear 22-Dec-2024 DEFINITION C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25] ACCESSION BBa_K1159102 VERSION BBa_K1159102.1 FEATURES Location/Qualifiers CDS 1..354 /label=C-terminal half of TEV Protease misc_feature 301..303 /label=Ser --> Val for autolysis resistance ORIGIN 1 aagagcatgt ctagcatggt gtcagacact agctgcacat tcccttcatc tgatggcata 61 ttctggaagc attggattca aaccaaggat gggcagtgtg gcagtccatt agtatcaact 121 agagatgggt tcattgttgg tatacactca gcatcgaatt tcaccaacac aaacaattat 181 ttcacaagcg tgccgaaaaa cttcatggaa ttgttgacaa atcaggaggc gcagcagtgg 241 gttagtggtt ggcgattaaa tgctgactca gtattgtggg ggggccataa agttttcatg 301 gtgaaacctg aagagccttt tcagccagtt aaggaagcga ctcaactcat gaat //