LOCUS       BBa_K1159106            3195 bp    DNA     linear       22-Dec-2024
DEFINITION  PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a 
            light-switchable TEV-Protease) in RFC[25]
ACCESSION   BBa_K1159106
VERSION     BBa_K1159106.1  
FEATURES             Location/Qualifiers
     misc_feature    325..327
                     /label=Essential Arg for photoconvertible binding of 
                     PIF3/6
     misc_feature    328..330
                     /label=Essential Gly for photoconvertible binding of 
                     PIF3/6
     misc_feature    1039..1041
                     /label=Essential Gly for photoconvertible binding of 
                     PIF3/6
     misc_feature    1051..1053
                     /label=Essential Arg for photoconvertible binding of 
                     PIF3/6
     misc_feature    1066..1068
                     /label=Essential Cys for chromophore attachement of 
                     phycocyanobilin/phytochromobilin
     CDS             1..2721
                     /label=PhyB (2-908 AA) photosensitive core
     misc_feature    1..2721
                     /label=BBa_K801031
     misc_feature    2722..2727
                     /label=25/25 scar
     misc_feature    2722..2727
                     /label=BBa_K1159993
     misc_feature    2728..2835
                     /label=GSAT Linker
     misc_feature    2728..2835
                     /label=BBa_K243029
     misc_feature    2836..2841
                     /label=25/25 scar
     misc_feature    2836..2841
                     /label=BBa_K1159993
     CDS             2842..3195
                     /label=C-terminal half of TEV Protease
     misc_feature    3142..3144
                     /label=Ser --> Val for autolysis resistance
     CDS             2842..3195
                     /label=BBa_K1159102
ORIGIN
        1 gtttccggag tcgggggtag tggcggtggc cgtggcggtg gccgtggcgg agaagaagaa
       61 ccgtcgtcaa gtcacactcc taataaccga agaggaggag aacaagctca atcgtcggga
      121 acgaaatctc tcagaccaag aagcaacact gaatcaatga gcaaagcaat tcaacagtac
      181 accgtcgacg caagactcca cgccgttttc gaacaatccg gcgaatcagg gaaatcattc
      241 gactactcac aatcactcaa aacgacgacg tacggttcct ctgtacctga gcaacagatc
      301 acagcttatc tctctcgaat ccagcgaggt ggttacattc agcctttcgg atgtatgatc
      361 gccgtcgatg aatccagttt ccggatcatc ggttacagtg aaaacgccag agaaatgtta
      421 gggattatgc ctcaatctgt tcctactctt gagaaacctg agattctagc tatgggaact
      481 gatgtgagat ctttgttcac ttcttcgagc tcgattctac tcgagcgtgc tttcgttgct
      541 cgagagatta ccttgttaaa tccggtttgg atccattcca agaatactgg taaaccgttt
      601 tacgccattc ttcataggat tgatgttggt gttgttattg atttagagcc agctagaact
      661 gaagatcctg cgctttctat tgctggtgct gttcaatcgc agaaactcgc ggttcgtgcg
      721 atttctcagt tacaggctct tcctggtgga gatattaagc ttttgtgtga cactgtcgtg
      781 gaaagtgtga gggacttgac tggttatgat cgtgttatgg tttataagtt tcatgaagat
      841 gagcatggag aagttgtagc tgagagtaaa cgagacgatt tagagcctta tattggactg
      901 cattatcctg ctactgatat tcctcaagcg tcaaggttct tgtttaagca gaaccgtgtc
      961 cgaatgatag tagattgcaa tgccacacct gttcttgtgg tccaggacga taggctaact
     1021 cagtctatgt gcttggttgg ttctactctt agggctcctc atggttgtca ctctcagtat
     1081 atggctaaca tgggatctat tgcgtcttta gcaatggcgg ttataatcaa tggaaatgaa
     1141 gatgatggga gcaatgtagc tagtggaaga agctcgatga ggctttgggg tttggttgtt
     1201 tgccatcaca cttcttctcg ctgcataccg tttccgctaa ggtatgcttg tgagtttttg
     1261 atgcaggctt tcggtttaca gttaaacatg gaattgcagt tagctttgca aatgtcagag
     1321 aaacgcgttt tgagaacgca gacactgtta tgtgatatgc ttctgcgtga ctcgcctgct
     1381 ggaattgtta cacagagtcc cagtatcatg gacttagtga aatgtgacgg tgcagcattt
     1441 ctttaccacg ggaagtatta cccgttgggt gttgctccta gtgaagttca gataaaagat
     1501 gttgtggagt ggttgcttgc gaatcatgcg gattcaaccg gattaagcac tgatagttta
     1561 ggcgatgcgg ggtatcccgg tgcagctgcg ttaggggatg ctgtgtgcgg tatggcagtt
     1621 gcatatatca caaaaagaga ctttcttttt tggtttcgat ctcacactgc gaaagaaatc
     1681 aaatggggag gcgctaagca tcatccggag gataaagatg atgggcaacg aatgcatcct
     1741 cgttcgtcct ttcaggcttt tcttgaagtt gttaagagcc ggagtcagcc atgggaaact
     1801 gcggaaatgg atgcgattca ctcgctccag cttattctga gagactcttt taaagaatct
     1861 gaggcggcta tgaactctaa agttgtggat ggtgtggttc agccatgtag ggatatggcg
     1921 ggggaacagg ggattgatga gttaggtgca gttgcaagag agatggttag gctcattgag
     1981 actgcaactg ttcctatatt cgctgtggat gccggaggct gcatcaatgg atggaacgct
     2041 aagattgcag agttgacagg tctctcagtt gaagaagcta tggggaagtc tctggtttct
     2101 gatttaatat acaaagagaa tgaagcaact gtcaataagc ttctttctcg tgctttgaga
     2161 ggggacgagg aaaagaatgt ggaggttaag ctgaaaactt tcagccccga actacaaggg
     2221 aaagcagttt ttgtggttgt gaatgcttgt tccagcaagg actacttgaa caacattgtc
     2281 ggcgtttgtt ttgttggaca agacgttacg agtcagaaaa tcgtaatgga taagttcatc
     2341 aacatacaag gagattacaa ggctattgta catagcccaa accctctaat cccgccaatt
     2401 tttgctgctg acgagaacac gtgctgcctg gaatggaaca tggcgatgga aaagcttacg
     2461 ggttggtctc gcagtgaagt gattgggaaa atgattgtcg gggaagtgtt tgggagctgt
     2521 tgcatgctaa agggtcctga tgctttaacc aagttcatga ttgtattgca taatgcgatt
     2581 ggtggccaag atacggataa gttccctttc ccattctttg accgcaatgg gaagtttgtt
     2641 caggctctat tgactgcaaa caagcgggtt agcctcgagg gaaaggttat tggggctttc
     2701 tgtttcttgc aaatcccgag caccggcggt ggttctgccg gtggctccgg ttctggctcc
     2761 agcggtggca gctctggtgc gtccggcacg ggtactgcgg gtggcactgg cagcggttcc
     2821 ggtactggct ctggcaccgg caagagcatg tctagcatgg tgtcagacac tagctgcaca
     2881 ttcccttcat ctgatggcat attctggaag cattggattc aaaccaagga tgggcagtgt
     2941 ggcagtccat tagtatcaac tagagatggg ttcattgttg gtatacactc agcatcgaat
     3001 ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa acttcatgga attgttgaca
     3061 aatcaggagg cgcagcagtg ggttagtggt tggcgattaa atgctgactc agtattgtgg
     3121 gggggccata aagttttcat ggtgaaacct gaagagcctt ttcagccagt taaggaagcg
     3181 actcaactca tgaat
//