LOCUS       BBa_K1159110             618 bp    DNA     linear       22-Nov-2024
DEFINITION  Thermonuclease with N-terminal SV40 NLS and flexible linker with 
            TEV site in RFC[25]
ACCESSION   BBa_K1159110
VERSION     BBa_K1159110.1  
FEATURES             Location/Qualifiers
     misc_feature    79..99
                     /label=TEV cleavage site
     misc_feature    1..75
                     /label=(GlyGlyGlySer)x5
     misc_feature    1..102
                     /label=BBa_K1159310
     misc_feature    103..108
                     /label=25/25 scar
     misc_feature    103..108
                     /label=BBa_K1159993
     misc_feature    130..150
                     /label=SV40 nuclear localization sequence (recognized by 
                     importin alpha)
     misc_feature    109..165
                     /label=BBa_K801030
     misc_feature    166..171
                     /label=25/25 scar
     misc_feature    166..171
                     /label=BBa_K1159993
     CDS             172..618
                     /label=Mature Thermonuclease (NucA)
     CDS             172..618
                     /label=BBa_K1159105
     CDS             109..618
                     /label=BBa_K1159109
ORIGIN
        1 ggtggaggcg gttcaggcgg aggtggctct ggcggtggcg gatcgggagg cggtggctcc
       61 ggaggtggag gctcaggaga gaatttgtat tttcagtcag gaaccggcgc ggaattaatt
      121 cccgagcctc caaaaaagaa gagaaaggtc gaattgggta ccgccaccgg cgcaacttca
      181 actaaaaaat tacataaaga acctgcgact ttaattaaag cgattgatgg tgatacggtt
      241 aaattaatgt acaaaggtca accaatgaca ttcagactat tattggttga tacacctgaa
      301 acaaagcatc ctaaaaaagg tgtagagaaa tatggtcctg aagcaagtgc atttacgaaa
      361 aaaatggtag aaaatgcaaa gaaaattgaa gtcgagtttg acaaaggtca aagaactgat
      421 aaatatggac gtggcttagc gtatatttat gctgatggaa aaatggtaaa cgaagcttta
      481 gttcgtcaag gcttggctaa agttgcttat gtttacaaac ctaacaatac acatgaacaa
      541 catttaagaa aaagtgaagc acaagcgaaa aaagagaaat taaatatttg gagcgaagac
      601 aacgctgatt caggtcaa
//