LOCUS       BBa_K1159111             916 bp    DNA     linear       17-May-2024
DEFINITION  Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS 
            in RFC[25] N-Part
ACCESSION   BBa_K1159111
VERSION     BBa_K1159111.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for ''P. patens''
     CDS             5..100
                     /label=SERK Signal Peptide
     misc_feature    1..100
                     /label=BBa_K1159303
     misc_feature    101..106
                     /label=25/25 scar
     misc_feature    101..106
                     /label=BBa_K1159993
     CDS             107..292
                     /label=Transmembrane Domain of SERK Receptor from ''P. 
                     patens''
     misc_feature    107..292
                     /label=BBa_K1159305
     CDS             1..292
                     /label=BBa_K1159314
     misc_feature    293..298
                     /label=25/25 scar
     misc_feature    293..298
                     /label=BBa_K1159993
     misc_feature    377..397
                     /label=TEV cleavage site
     misc_feature    299..373
                     /label=(GlyGlyGlySer)x5
     misc_feature    299..400
                     /label=BBa_K1159310
     misc_feature    401..406
                     /label=25/25 scar
     misc_feature    401..406
                     /label=BBa_K1159993
     misc_feature    428..448
                     /label=SV40 nuclear localization sequence (recognized by 
                     importin alpha)
     misc_feature    407..463
                     /label=BBa_K801030
     misc_feature    464..469
                     /label=25/25 scar
     misc_feature    464..469
                     /label=BBa_K1159993
     CDS             470..916
                     /label=Mature Thermonuclease (NucA)
     CDS             470..916
                     /label=BBa_K1159105
     CDS             407..916
                     /label=BBa_K1159109
     CDS             299..916
                     /label=BBa_K1159110
ORIGIN
        1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat
       61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggccctg gacaacctcc
      121 ttttcctcct cctcctcctt ttacaccacc tcctccacaa actcctaacg gtgcttctgg
      181 tgagaactct actggtgcta ttgctggtgg tgttgctgct ggtgcagctc ttttgtttgc
      241 tgctcctgct attggattcg cttggtggag aagaagaagg cctattgagg ctaccggcgg
      301 tggaggcggt tcaggcggag gtggctctgg cggtggcgga tcgggaggcg gtggctccgg
      361 aggtggaggc tcaggagaga atttgtattt tcagtcagga accggcgcgg aattaattcc
      421 cgagcctcca aaaaagaaga gaaaggtcga attgggtacc gccaccggcg caacttcaac
      481 taaaaaatta cataaagaac ctgcgacttt aattaaagcg attgatggtg atacggttaa
      541 attaatgtac aaaggtcaac caatgacatt cagactatta ttggttgata cacctgaaac
      601 aaagcatcct aaaaaaggtg tagagaaata tggtcctgaa gcaagtgcat ttacgaaaaa
      661 aatggtagaa aatgcaaaga aaattgaagt cgagtttgac aaaggtcaaa gaactgataa
      721 atatggacgt ggcttagcgt atatttatgc tgatggaaaa atggtaaacg aagctttagt
      781 tcgtcaaggc ttggctaaag ttgcttatgt ttacaaacct aacaatacac atgaacaaca
      841 tttaagaaaa agtgaagcac aagcgaaaaa agagaaatta aatatttgga gcgaagacaa
      901 cgctgattca ggtcaa
//