BBa_K1159110
1
BBa_K1159110
Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]
2013-09-15T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS) and a flexible linker with a TEV cleavage site. Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
component2347172
1
BBa_K1159109
component2347165
1
BBa_K1159993
component2347163
1
BBa_K1159310
annotation2347165
1
BBa_K1159993
range2347165
1
103
108
annotation2347172
1
BBa_K1159109
range2347172
1
109
618
annotation2347163
1
BBa_K1159310
range2347163
1
1
102
BBa_K1159993
1
BBa_K1159993
25/25 Assembly Scar
2013-09-14T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
false
false
_1471_
0
11507
9
Not in stock
false
x
false
TU Munich 2013
annotation2342863
1
25/25 scar
range2342863
1
1
6
BBa_K1159111
1
BBa_K1159111
Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part
2013-09-15T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
This part encodes a membrane-associated Thermonuclease NucA for the chassis ''Physcomitrella patens''. NucA is a endo/exonuclease that degrades ssDNA/dsDNA/ssRNA/dsRNA. It cannot execute its deadly potential when it is bound to the cytoplasmatic membrane. The region between NucA and the transmembrane domain consists a long linker with a TEV cleavage site and a SV40 nuclear localization signal (NLS). By cleaving the TEV cleavage site with a TEV Protease the nuclease NucA translocates in the nucleus due to its SV40 NLS and degrades the genomic DNA resulting a inevitable induction of programmed cell-death (PCD). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
component2347345
1
BBa_K1159993
component2347343
1
BBa_K1159314
component2347358
1
BBa_K1159110
annotation2347345
1
BBa_K1159993
range2347345
1
293
298
annotation2347358
1
BBa_K1159110
range2347358
1
299
916
annotation2347343
1
BBa_K1159314
range2347343
1
1
292
BBa_K1159314
1
BBa_K1159314
TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
2013-09-15T11:00:00Z
2015-06-11T01:53:32Z
Synthetic
This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25]. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
false
false
_1471_
4206
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
component2347281
1
BBa_K1159303
component2347285
1
BBa_K1159305
component2347283
1
BBa_K1159993
annotation2347281
1
BBa_K1159303
range2347281
1
1
100
annotation2347283
1
BBa_K1159993
range2347283
1
101
106
annotation2347285
1
BBa_K1159305
range2347285
1
107
292
BBa_K1159105
1
BBa_K1159105
Mature Nuclease NucA from <i>Staphylococcus aureus</i> (Thermonuclease) in RFC[25]
2013-09-13T11:00:00Z
2015-05-08T01:09:31Z
''Staphylococcus aureus''
Thermonuclease is a endo-exonuclease from ''Staphylococcus aureus'' that degrades dsDNA, ssDNA, dsRNA and ssRNA. This part is coding the mature form of the nuclease, also called NucA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
annotation2343335
1
Mature Thermonuclease (NucA)
range2343335
1
1
447
BBa_K1159305
1
BBa_K1159305
Transmembrane domain of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]
2013-09-13T11:00:00Z
2015-06-11T01:54:11Z
''Physcomitrella patens''
This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
false
false
_1471_
4206
11507
9
In stock
false
x
false
TU Munich 2013
annotation2343588
1
Transmembrane Domain of SERK Receptor from ''P. patens''
range2343588
1
1
186
BBa_K1159109
1
BBa_K1159109
Micrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]
2013-09-15T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS). Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
component2347156
1
BBa_K801030
component2347160
1
BBa_K1159105
component2347158
1
BBa_K1159993
annotation2347156
1
BBa_K801030
range2347156
1
1
57
annotation2347158
1
BBa_K1159993
range2347158
1
58
63
annotation2347160
1
BBa_K1159105
range2347160
1
64
510
BBa_K801030
1
BBa_K801030
Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
2012-09-20T11:00:00Z
2015-05-08T01:13:24Z
designed by oligo hybridisation based on plasmid "pGADT7 AD" from Clontech, w/ RFC25-pre and suffix for protein fusions
SV40NLS nuclear localization sequence
false
false
_1057_
0
11507
9
In stock
false
-
false
Dong-Jiunn Jeffery TRUONG
annotation2198843
1
SV40 nuclear localization sequence (recognized by importin alpha)
range2198843
1
22
42
BBa_K1159303
1
BBa_K1159303
Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25]
2013-09-13T11:00:00Z
2015-06-11T01:54:49Z
''Physcomitrella patens''
This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
false
false
_1471_
4206
11507
9
In stock
false
x
false
TU Munich 2013
annotation2341512
1
Consensus Sequence for ''P. patens''
range2341512
1
1
4
annotation2341513
1
SERK Signal Peptide
range2341513
1
5
100
BBa_K1159310
1
BBa_K1159310
34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]
2013-09-14T11:00:00Z
2015-05-08T01:09:31Z
Synthetic
This part encodes a 34 amino acids long flexible linker ((GlyGlyGlyGlySer)x5) with a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
false
false
_1471_
0
11507
9
In stock
false
x
false
Dong-Jiunn Jeffery TRUONG
annotation2342592
1
TEV cleavage site
range2342592
1
79
99
annotation2342593
1
(GlyGlyGlySer)x5
range2342593
1
1
75
BBa_K801030_sequence
1
gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgcc
BBa_K1159993_sequence
1
accggc
BBa_K1159303_sequence
1
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc
BBa_K1159110_sequence
1
ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
BBa_K1159109_sequence
1
gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
BBa_K1159305_sequence
1
cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
BBa_K1159105_sequence
1
gcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
BBa_K1159314_sequence
1
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
BBa_K1159111_sequence
1
aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
BBa_K1159310_sequence
1
ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcagga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z