BBa_K1159111
type: ComponentDefinition
persistent identity: BBa K1159111
display id: BBa_K1159111
version: 1
was generated by: iGEM to SBOL conversion
description: Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a membrane-associated Thermonuclease NucA for the chassis ''Physcomitrella patens''. NucA is a endo/exonuclease that degrades ssDNA/dsDNA/ssRNA/dsRNA. It cannot execute its deadly potential when it is bound to the cytoplasmatic membrane. The region between NucA and the transmembrane domain consists a long linker with a TEV cleavage site and a SV40 nuclear localization signal (NLS). By cleaving the TEV cleavage site with a TEV Protease the nuclease NucA translocates in the nucleus due to its SV40 NLS and degrades the genomic DNA resulting a inevitable induction of programmed cell-death (PCD). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159110
component: BBa_K1159993
component: BBa_K1159314
sequence annotation: BBa_K1159314
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159110
sequence: https://synbiohub.org/public/igem/BBa_K1159111_sequence/1

BBa_K1159110
type: Component
persistent identity: component2347358
display id: component2347358
version: 1
top level: BBa_K1159111
definition: BBa_K1159110
access: public

BBa_K1159993
type: Component
persistent identity: component2347345
display id: component2347345
version: 1
top level: BBa_K1159111
definition: BBa_K1159993
access: public

BBa_K1159314
type: Component
persistent identity: component2347343
display id: component2347343
version: 1
top level: BBa_K1159111
definition: BBa_K1159314
access: public

BBa_K1159314
type: SequenceAnnotation
persistent identity: annotation2347343
display id: annotation2347343
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347343/range2347343/1
component: BBa_K1159314

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347343/range2347343/1
type: Range
persistent identity: range2347343
display id: range2347343
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 1
end: 292
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347345
display id: annotation2347345
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347345/range2347345/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347345/range2347345/1
type: Range
persistent identity: range2347345
display id: range2347345
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 293
end: 298
orientation: inline

BBa_K1159110
type: SequenceAnnotation
persistent identity: annotation2347358
display id: annotation2347358
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347358/range2347358/1
component: BBa_K1159110

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347358/range2347358/1
type: Range
persistent identity: range2347358
display id: range2347358
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 299
end: 916
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159110
type: ComponentDefinition
persistent identity: BBa K1159110
display id: BBa_K1159110
version: 1
was generated by: iGEM to SBOL conversion
description: Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS) and a flexible linker with a TEV cleavage site. Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159109
component: BBa_K1159993
component: BBa_K1159310
sequence annotation: BBa_K1159310
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159109
sequence: https://synbiohub.org/public/igem/BBa_K1159110_sequence/1

BBa_K1159109
type: Component
persistent identity: component2347172
display id: component2347172
version: 1
top level: BBa_K1159110
definition: BBa_K1159109
access: public

BBa_K1159993
type: Component
persistent identity: component2347165
display id: component2347165
version: 1
top level: BBa_K1159110
definition: BBa_K1159993
access: public

BBa_K1159310
type: Component
persistent identity: component2347163
display id: component2347163
version: 1
top level: BBa_K1159110
definition: BBa_K1159310
access: public

BBa_K1159310
type: SequenceAnnotation
persistent identity: annotation2347163
display id: annotation2347163
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347163/range2347163/1
component: BBa_K1159310

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347163/range2347163/1
type: Range
persistent identity: range2347163
display id: range2347163
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 1
end: 102
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347165
display id: annotation2347165
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347165/range2347165/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347165/range2347165/1
type: Range
persistent identity: range2347165
display id: range2347165
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 103
end: 108
orientation: inline

BBa_K1159109
type: SequenceAnnotation
persistent identity: annotation2347172
display id: annotation2347172
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347172/range2347172/1
component: BBa_K1159109

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347172/range2347172/1
type: Range
persistent identity: range2347172
display id: range2347172
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 109
end: 618
orientation: inline

BBa_K1159314
type: ComponentDefinition
persistent identity: BBa K1159314
display id: BBa_K1159314
version: 1
was generated by: iGEM to SBOL conversion
description: TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:32Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25]. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159305
component: BBa_K1159993
component: BBa_K1159303
sequence annotation: BBa_K1159303
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159305
sequence: https://synbiohub.org/public/igem/BBa_K1159314_sequence/1

BBa_K1159305
type: Component
persistent identity: component2347285
display id: component2347285
version: 1
top level: BBa_K1159314
definition: BBa_K1159305
access: public

BBa_K1159993
type: Component
persistent identity: component2347283
display id: component2347283
version: 1
top level: BBa_K1159314
definition: BBa_K1159993
access: public

BBa_K1159303
type: Component
persistent identity: component2347281
display id: component2347281
version: 1
top level: BBa_K1159314
definition: BBa_K1159303
access: public

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2347281
display id: annotation2347281
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
type: Range
persistent identity: range2347281
display id: range2347281
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347283
display id: annotation2347283
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
type: Range
persistent identity: range2347283
display id: range2347283
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 101
end: 106
orientation: inline

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2347285
display id: annotation2347285
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347285/range2347285/1
component: BBa_K1159305

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347285/range2347285/1
type: Range
persistent identity: range2347285
display id: range2347285
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 107
end: 292
orientation: inline

BBa_K1159303
type: ComponentDefinition
persistent identity: BBa K1159303
display id: BBa_K1159303
version: 1
was generated by: iGEM to SBOL conversion
description: Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:49Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Consensus Sequence for ''P. patens''
sequence annotation: SERK Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159303_sequence/1

Consensus Sequence for ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2341512
display id: annotation2341512
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
type: Range
persistent identity: range2341512
display id: range2341512
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 1
end: 4
orientation: inline

SERK Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341513
display id: annotation2341513
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
type: Range
persistent identity: range2341513
display id: range2341513
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 5
end: 100
orientation: inline

BBa_K1159305
type: ComponentDefinition
persistent identity: BBa K1159305
display id: BBa_K1159305
version: 1
was generated by: iGEM to SBOL conversion
description: Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:11Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Transmembrane Domain of SERK Receptor from ''P. patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159305_sequence/1

Transmembrane Domain of SERK Receptor from ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2343588
display id: annotation2343588
version: 1
top level: BBa_K1159305
location: https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
type: Range
persistent identity: range2343588
display id: range2343588
version: 1
top level: BBa_K1159305
direction: direction/forward
start: 1
end: 186
orientation: inline

BBa_K1159310
type: ComponentDefinition
persistent identity: BBa K1159310
display id: BBa_K1159310
version: 1
was generated by: iGEM to SBOL conversion
description: 34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a 34 amino acids long flexible linker ((GlyGlyGlyGlySer)x5) with a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: (GlyGlyGlySer)x5
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1159310_sequence/1

(GlyGlyGlySer)x5
type: SequenceAnnotation
persistent identity: annotation2342593
display id: annotation2342593
version: 1
top level: BBa_K1159310
location: https://synbiohub.org/public/igem/BBa_K1159310/annotation2342593/range2342593/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159310/annotation2342593/range2342593/1
type: Range
persistent identity: range2342593
display id: range2342593
version: 1
top level: BBa_K1159310
direction: direction/forward
start: 1
end: 75
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2342592
display id: annotation2342592
version: 1
top level: BBa_K1159310
location: https://synbiohub.org/public/igem/BBa_K1159310/annotation2342592/range2342592/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159310/annotation2342592/range2342592/1
type: Range
persistent identity: range2342592
display id: range2342592
version: 1
top level: BBa_K1159310
direction: direction/forward
start: 79
end: 99
orientation: inline

BBa_K1159109
type: ComponentDefinition
persistent identity: BBa K1159109
display id: BBa_K1159109
version: 1
was generated by: iGEM to SBOL conversion
description: Micrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS). Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159105
component: BBa_K1159993
component: BBa_K801030
sequence annotation: BBa_K801030
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159105
sequence: https://synbiohub.org/public/igem/BBa_K1159109_sequence/1

BBa_K1159105
type: Component
persistent identity: component2347160
display id: component2347160
version: 1
top level: BBa_K1159109
definition: BBa_K1159105
access: public

BBa_K1159993
type: Component
persistent identity: component2347158
display id: component2347158
version: 1
top level: BBa_K1159109
definition: BBa_K1159993
access: public

BBa_K801030
type: Component
persistent identity: component2347156
display id: component2347156
version: 1
top level: BBa_K1159109
definition: BBa_K801030
access: public

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2347156
display id: annotation2347156
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347156/range2347156/1
component: BBa_K801030

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347156/range2347156/1
type: Range
persistent identity: range2347156
display id: range2347156
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347158
display id: annotation2347158
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347158/range2347158/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347158/range2347158/1
type: Range
persistent identity: range2347158
display id: range2347158
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 58
end: 63
orientation: inline

BBa_K1159105
type: SequenceAnnotation
persistent identity: annotation2347160
display id: annotation2347160
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347160/range2347160/1
component: BBa_K1159105

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347160/range2347160/1
type: Range
persistent identity: range2347160
display id: range2347160
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 64
end: 510
orientation: inline

BBa_K1159105
type: ComponentDefinition
persistent identity: BBa K1159105
display id: BBa_K1159105
version: 1
was generated by: iGEM to SBOL conversion
description: Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Staphylococcus aureus''
owned by: james
owned by: myers
mutable description: Thermonuclease is a endo-exonuclease from ''Staphylococcus aureus'' that degrades dsDNA, ssDNA, dsRNA and ssRNA. This part is coding the mature form of the nuclease, also called NucA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Mature Thermonuclease (NucA)
sequence: https://synbiohub.org/public/igem/BBa_K1159105_sequence/1

Mature Thermonuclease (NucA)
type: SequenceAnnotation
persistent identity: annotation2343335
display id: annotation2343335
version: 1
top level: BBa_K1159105
location: https://synbiohub.org/public/igem/BBa_K1159105/annotation2343335/range2343335/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159105/annotation2343335/range2343335/1
type: Range
persistent identity: range2343335
display id: range2343335
version: 1
top level: BBa_K1159105
direction: direction/forward
start: 1
end: 447
orientation: inline

BBa_K801030
type: ComponentDefinition
persistent identity: BBa K801030
display id: BBa_K801030
version: 1
was generated by: iGEM to SBOL conversion
description: Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: designed by oligo hybridisation based on plasmid "pGADT7 AD" from Clontech, w/ RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
mutable description: SV40NLS nuclear localization sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence: https://synbiohub.org/public/igem/BBa_K801030_sequence/1

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2198843
display id: annotation2198843
version: 1
top level: BBa_K801030
location: https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
type: Range
persistent identity: range2198843
display id: range2198843
version: 1
top level: BBa_K801030
direction: direction/forward
start: 22
end: 42
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159111_sequence/1
type: Sequence
persistent identity: BBa K1159111 sequence
display id: BBa_K1159111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159110_sequence/1
type: Sequence
persistent identity: BBa K1159110 sequence
display id: BBa_K1159110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159314_sequence/1
type: Sequence
persistent identity: BBa K1159314 sequence
display id: BBa_K1159314_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159303_sequence/1
type: Sequence
persistent identity: BBa K1159303 sequence
display id: BBa_K1159303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159305_sequence/1
type: Sequence
persistent identity: BBa K1159305 sequence
display id: BBa_K1159305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159310_sequence/1
type: Sequence
persistent identity: BBa K1159310 sequence
display id: BBa_K1159310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcagga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159109_sequence/1
type: Sequence
persistent identity: BBa K1159109 sequence
display id: BBa_K1159109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159105_sequence/1
type: Sequence
persistent identity: BBa K1159105 sequence
display id: BBa_K1159105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801030_sequence/1
type: Sequence
persistent identity: BBa K801030 sequence
display id: BBa_K801030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z