LOCUS       BBa_K1159112            1467 bp    DNA     linear       06-Dec-2025
DEFINITION  FRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2
            ) in RFC[25]
ACCESSION   BBa_K1159112
VERSION     BBa_K1159112.1  
FEATURES             Location/Qualifiers
     CDS             1..714
                     /label=eCFP
     CDS             1..714
                     /label=BBa_K1159302
     misc_feature    715..720
                     /label=25/25 scar
     misc_feature    715..720
                     /label=BBa_K1159993
     misc_feature    724..744
                     /label=TEV cleavage site
     CDS             721..747
                     /label=BBa_K1159318
     misc_feature    748..753
                     /label=25/25 scar
     misc_feature    748..753
                     /label=BBa_K1159993
     CDS             754..1467
                     /label=SYFP2
     CDS             754..1467
                     /label=BBa_K1159301
ORIGIN
        1 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
       61 gacgtgaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      181 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      421 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
      481 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      601 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaccggc
      721 ggagagaatt tgtattttca gtcaggaacc ggcgttagca agggcgaaga actttttaca
      781 ggcgtagtac cgatcttagt tgaattagac ggcgacgtta acggtcataa gtttagcgtg
      841 agcggtgagg gtgaaggtga cgcaacttac ggcaagctga ccctgaagct gatttgcacg
      901 acgggtaagc tgccggtccc gtggcctacc ctggtcacga ccttgggtta tggcgttcag
      961 tgtttcgcgc gttatccgga ccacatgaaa caacacgatt tctttaagag cgcgatgcca
     1021 gaaggctatg tgcaggagcg tacgatcttt ttcaaagacg acggtaacta caagacgcgt
     1081 gccgaagtca aattcgaagg cgacaccctg gtgaatcgca ttgagctgaa gggtattgat
     1141 ttcaaagagg atggcaatat cctgggtcac aagctggagt acaattacaa ttcccacaac
     1201 gtttacatca ccgcagataa acagaaaaat ggcatcaaag cgaatttcaa aatccgtcac
     1261 aacattgagg acggtggtgt tcaactggcg gatcattacc agcaaaacac cccgattggt
     1321 gacggtccgg tcctgttgcc ggataaccat tatctgtctt accaaagcaa actgagcaaa
     1381 gatccgaacg agaagcgcga ccacatggtg ctgctggagt ttgtgaccgc tgccggtatt
     1441 accctgggta tggatgagct gtataaa
//