LOCUS BBa_K1159113 4625 bp DNA linear 22-Nov-2024 DEFINITION Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based) ACCESSION BBa_K1159113 VERSION BBa_K1159113.1 FEATURES Location/Qualifiers misc_feature 325..327 /label=Essential Arg for photoconvertible binding of PIF3/6 misc_feature 328..330 /label=Essential Gly for photoconvertible binding of PIF3/6 misc_feature 1039..1041 /label=Essential Gly for photoconvertible binding of PIF3/6 misc_feature 1051..1053 /label=Essential Arg for photoconvertible binding of PIF3/6 misc_feature 1066..1068 /label=Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin CDS 1..2721 /label=PhyB (2-908 AA) photosensitive core misc_feature 1..2721 /label=BBa_K801031 misc_feature 2722..2727 /label=25/25 scar misc_feature 2722..2727 /label=BBa_K1159993 misc_feature 2728..2835 /label=GSAT Linker misc_feature 2728..2835 /label=BBa_K243029 misc_feature 2836..2841 /label=25/25 scar misc_feature 2836..2841 /label=BBa_K1159993 CDS 2842..3195 /label=C-terminal half of TEV Protease misc_feature 3142..3144 /label=Ser --> Val for autolysis resistance CDS 2842..3195 /label=BBa_K1159102 CDS 1..3195 /label=BBa_K1159106 misc_feature 3196..3212 /label=25/10 NC scar misc_feature 3196..3212 /label=BBa_K1159996 RBS 3213..3839 /label=Polioviral IRES RBS 3213..3839 /label=BBa_K1159300 misc_feature 3840..3854 /label=10/25 scar misc_feature 3840..3854 /label=BBa_K1159994 CDS 3855..4208 /label=N-terminal half of TEV Protease CDS 3855..4208 /label=BBa_K1159101 misc_feature 4209..4214 /label=25/25 scar misc_feature 4209..4214 /label=BBa_K1159993 misc_feature 4215..4322 /label=GSAT Linker misc_feature 4215..4322 /label=BBa_K243029 misc_feature 4323..4328 /label=25/25 scar misc_feature 4323..4328 /label=BBa_K1159993 CDS 4329..4625 /label=PIF3 (2-100 AA) CDS 4329..4625 /label=BBa_K1159103 CDS 3855..4625 /label=BBa_K1159107 ORIGIN 1 gtttccggag tcgggggtag tggcggtggc cgtggcggtg gccgtggcgg agaagaagaa 61 ccgtcgtcaa gtcacactcc taataaccga agaggaggag aacaagctca atcgtcggga 121 acgaaatctc tcagaccaag aagcaacact gaatcaatga gcaaagcaat tcaacagtac 181 accgtcgacg caagactcca cgccgttttc gaacaatccg gcgaatcagg gaaatcattc 241 gactactcac aatcactcaa aacgacgacg tacggttcct ctgtacctga gcaacagatc 301 acagcttatc tctctcgaat ccagcgaggt ggttacattc agcctttcgg atgtatgatc 361 gccgtcgatg aatccagttt ccggatcatc ggttacagtg aaaacgccag agaaatgtta 421 gggattatgc ctcaatctgt tcctactctt gagaaacctg agattctagc tatgggaact 481 gatgtgagat ctttgttcac ttcttcgagc tcgattctac tcgagcgtgc tttcgttgct 541 cgagagatta ccttgttaaa tccggtttgg atccattcca agaatactgg taaaccgttt 601 tacgccattc ttcataggat tgatgttggt gttgttattg atttagagcc agctagaact 661 gaagatcctg cgctttctat tgctggtgct gttcaatcgc agaaactcgc ggttcgtgcg 721 atttctcagt tacaggctct tcctggtgga gatattaagc ttttgtgtga cactgtcgtg 781 gaaagtgtga gggacttgac tggttatgat cgtgttatgg tttataagtt tcatgaagat 841 gagcatggag aagttgtagc tgagagtaaa cgagacgatt tagagcctta tattggactg 901 cattatcctg ctactgatat tcctcaagcg tcaaggttct tgtttaagca gaaccgtgtc 961 cgaatgatag tagattgcaa tgccacacct gttcttgtgg tccaggacga taggctaact 1021 cagtctatgt gcttggttgg ttctactctt agggctcctc atggttgtca ctctcagtat 1081 atggctaaca tgggatctat tgcgtcttta gcaatggcgg ttataatcaa tggaaatgaa 1141 gatgatggga gcaatgtagc tagtggaaga agctcgatga ggctttgggg tttggttgtt 1201 tgccatcaca cttcttctcg ctgcataccg tttccgctaa ggtatgcttg tgagtttttg 1261 atgcaggctt tcggtttaca gttaaacatg gaattgcagt tagctttgca aatgtcagag 1321 aaacgcgttt tgagaacgca gacactgtta tgtgatatgc ttctgcgtga ctcgcctgct 1381 ggaattgtta cacagagtcc cagtatcatg gacttagtga aatgtgacgg tgcagcattt 1441 ctttaccacg ggaagtatta cccgttgggt gttgctccta gtgaagttca gataaaagat 1501 gttgtggagt ggttgcttgc gaatcatgcg gattcaaccg gattaagcac tgatagttta 1561 ggcgatgcgg ggtatcccgg tgcagctgcg ttaggggatg ctgtgtgcgg tatggcagtt 1621 gcatatatca caaaaagaga ctttcttttt tggtttcgat ctcacactgc gaaagaaatc 1681 aaatggggag gcgctaagca tcatccggag gataaagatg atgggcaacg aatgcatcct 1741 cgttcgtcct ttcaggcttt tcttgaagtt gttaagagcc ggagtcagcc atgggaaact 1801 gcggaaatgg atgcgattca ctcgctccag cttattctga gagactcttt taaagaatct 1861 gaggcggcta tgaactctaa agttgtggat ggtgtggttc agccatgtag ggatatggcg 1921 ggggaacagg ggattgatga gttaggtgca gttgcaagag agatggttag gctcattgag 1981 actgcaactg ttcctatatt cgctgtggat gccggaggct gcatcaatgg atggaacgct 2041 aagattgcag agttgacagg tctctcagtt gaagaagcta tggggaagtc tctggtttct 2101 gatttaatat acaaagagaa tgaagcaact gtcaataagc ttctttctcg tgctttgaga 2161 ggggacgagg aaaagaatgt ggaggttaag ctgaaaactt tcagccccga actacaaggg 2221 aaagcagttt ttgtggttgt gaatgcttgt tccagcaagg actacttgaa caacattgtc 2281 ggcgtttgtt ttgttggaca agacgttacg agtcagaaaa tcgtaatgga taagttcatc 2341 aacatacaag gagattacaa ggctattgta catagcccaa accctctaat cccgccaatt 2401 tttgctgctg acgagaacac gtgctgcctg gaatggaaca tggcgatgga aaagcttacg 2461 ggttggtctc gcagtgaagt gattgggaaa atgattgtcg gggaagtgtt tgggagctgt 2521 tgcatgctaa agggtcctga tgctttaacc aagttcatga ttgtattgca taatgcgatt 2581 ggtggccaag atacggataa gttccctttc ccattctttg accgcaatgg gaagtttgtt 2641 caggctctat tgactgcaaa caagcgggtt agcctcgagg gaaaggttat tggggctttc 2701 tgtttcttgc aaatcccgag caccggcggt ggttctgccg gtggctccgg ttctggctcc 2761 agcggtggca gctctggtgc gtccggcacg ggtactgcgg gtggcactgg cagcggttcc 2821 ggtactggct ctggcaccgg caagagcatg tctagcatgg tgtcagacac tagctgcaca 2881 ttcccttcat ctgatggcat attctggaag cattggattc aaaccaagga tgggcagtgt 2941 ggcagtccat tagtatcaac tagagatggg ttcattgttg gtatacactc agcatcgaat 3001 ttcaccaaca caaacaatta tttcacaagc gtgccgaaaa acttcatgga attgttgaca 3061 aatcaggagg cgcagcagtg ggttagtggt tggcgattaa atgctgactc agtattgtgg 3121 gggggccata aagttttcat ggtgaaacct gaagagcctt ttcagccagt taaggaagcg 3181 actcaactca tgaataccgg ttaatactag agttaaaaca gctctggggt tgtacccacc 3241 ccagaggccc acgtggcggc tagtactccg gtattgcggt acccttgtac gcctgtttta 3301 tactcccttc ccgtaactta gacgcacaaa accaagttca atagaagggg gtacaaacca 3361 gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt cgtatagact gcttgcgtgg 3421 ttgaaagcga cggatccgtt atccgcttat gtacttcgag aagcccagta ccacctcgga 3481 atcttcgatg cgttgcgctc agcactcaac cccagagtgt agcttaggct gatgagtctg 3541 gacatccctc accggtgacg gtggtccagg ctgcgttggc ggcctaccta tggctaacgc 3601 catgggacgc tagttgtgaa caaggtgtga agagcctatt gagctacata agaatcctcc 3661 ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg tcacaaacca gtgattggcc 3721 tgtcgtaacg cgcaagtccg tggcggaacc gactactttg ggtgtccgtg tttcctttta 3781 ttttattgtg gctgcttatg gtgacaatca cagattgtta tcataaagcg aattggattt 3841 actagatggc cggcggagaa agcttgttta aggggccgcg tgattacaac ccgatatcga 3901 gcaccatttg tcatttgacg aatgaatctg atgggcacac aacatcgttg tatggtattg 3961 gatttggtcc cttcatcatt acaaacaagc acttgtttag aagaaataat ggaacactgt 4021 tggtccaatc actacatggt gtattcaagg tcaagaacac cacgactttg caacaacacc 4081 tcattgatgg gagggacatg ataattattc gcatgcctaa ggatttccca ccatttcctc 4141 aaaagctgaa atttagagag ccacaaaggg aagagcgcat atgtcttgtg acaaccaact 4201 tccaaactac cggcggtggt tctgccggtg gctccggttc tggctccagc ggtggcagct 4261 ctggtgcgtc cggcacgggt actgcgggtg gcactggcag cggttccggt actggctctg 4321 gcaccggccc tctgtttgaa cttttcaggc tcaccaaagc taagcttgaa tctgctcaag 4381 acaggaaccc ttctccacct gtagatgaag ttgtggagct ggtgtgggaa aatggtcaga 4441 tatcaactca aagtcagtca agtagatcga ggaacattcc tccaccacaa gcaaactctt 4501 caagagctag agagattgga aatggctcaa agacgactat ggtggacgag atccctatgt 4561 cagtgccatc actaatgacg ggtttgagtc aagacgatga ctttgttcca tggttgaatc 4621 atcat //