BBa_K1159113
type: ComponentDefinition
persistent identity: BBa K1159113
display id: BBa_K1159113
version: 1
was generated by: iGEM to SBOL conversion
description: Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a light-switchable TEV Protease system consisting of two fusion proteins ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159107 BBa_K1159107]) seperated by an polioviral IRES [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159300 BBa_K1159300] which allows polycistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159107
component: BBa_K1159994
component: BBa_K1159300
component: BBa_K1159996
component: BBa_K1159106
sequence annotation: BBa_K1159106
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence annotation: BBa_K1159994
sequence annotation: BBa_K1159107
sequence: https://synbiohub.org/public/igem/BBa_K1159113_sequence/1

BBa_K1159107
type: Component
persistent identity: component2347568
display id: component2347568
version: 1
top level: BBa_K1159113
definition: BBa_K1159107
access: public

BBa_K1159994
type: Component
persistent identity: component2347557
display id: component2347557
version: 1
top level: BBa_K1159113
definition: BBa_K1159994
access: public

BBa_K1159300
type: Component
persistent identity: component2347555
display id: component2347555
version: 1
top level: BBa_K1159113
definition: BBa_K1159300
access: public

BBa_K1159996
type: Component
persistent identity: component2347553
display id: component2347553
version: 1
top level: BBa_K1159113
definition: BBa_K1159996
access: public

BBa_K1159106
type: Component
persistent identity: component2347551
display id: component2347551
version: 1
top level: BBa_K1159113
definition: BBa_K1159106
access: public

BBa_K1159106
type: SequenceAnnotation
persistent identity: annotation2347551
display id: annotation2347551
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347551/range2347551/1
component: BBa_K1159106

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347551/range2347551/1
type: Range
persistent identity: range2347551
display id: range2347551
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 1
end: 3195
orientation: inline

BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2347553
display id: annotation2347553
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347553/range2347553/1
component: BBa_K1159996

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347553/range2347553/1
type: Range
persistent identity: range2347553
display id: range2347553
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3196
end: 3212
orientation: inline

BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2347555
display id: annotation2347555
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347555/range2347555/1
component: BBa_K1159300

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347555/range2347555/1
type: Range
persistent identity: range2347555
display id: range2347555
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3213
end: 3839
orientation: inline

BBa_K1159994
type: SequenceAnnotation
persistent identity: annotation2347557
display id: annotation2347557
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347557/range2347557/1
component: BBa_K1159994

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347557/range2347557/1
type: Range
persistent identity: range2347557
display id: range2347557
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3840
end: 3854
orientation: inline

BBa_K1159107
type: SequenceAnnotation
persistent identity: annotation2347568
display id: annotation2347568
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347568/range2347568/1
component: BBa_K1159107

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347568/range2347568/1
type: Range
persistent identity: range2347568
display id: range2347568
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3855
end: 4625
orientation: inline

BBa_K1159300
type: ComponentDefinition
persistent identity: BBa K1159300
display id: BBa_K1159300
version: 1
was generated by: iGEM to SBOL conversion
description: Polioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotes
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:55:50Z
mutable provenance: Human Poliovirus 1 Mahoney Strain
owned by: james
owned by: myers
mutable description: This part contains the Internal Ribosome Entry Site (IRES) of the human poliovirus 1 Mahoney strain which allows polycistronic expression systems in eukaryotic systems. This part is flanked by RFC[10] pre- and suffix. By cloning this part betweens CDSs, many proteins/peptides can expressed by a single promoter and terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Polioviral IRES
sequence: https://synbiohub.org/public/igem/BBa_K1159300_sequence/1

Polioviral IRES
type: SequenceAnnotation
persistent identity: annotation2343585
display id: annotation2343585
version: 1
top level: BBa_K1159300
location: https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
type: Range
persistent identity: range2343585
display id: range2343585
version: 1
top level: BBa_K1159300
direction: direction/forward
start: 1
end: 627
orientation: inline

BBa_K1159994
type: ComponentDefinition
persistent identity: BBa K1159994
display id: BBa_K1159994
version: 1
was generated by: iGEM to SBOL conversion
description: 10/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] Biobrick (not RFC[25] N-part!).
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 10/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159994_sequence/1

10/25 scar
type: SequenceAnnotation
persistent identity: annotation2342865
display id: annotation2342865
version: 1
top level: BBa_K1159994
location: https://synbiohub.org/public/igem/BBa_K1159994/annotation2342865/range2342865/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159994/annotation2342865/range2342865/1
type: Range
persistent identity: range2342865
display id: range2342865
version: 1
top level: BBa_K1159994
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K1159996
type: ComponentDefinition
persistent identity: BBa K1159996
display id: BBa_K1159996
version: 1
was generated by: iGEM to SBOL conversion
description: 25/10 NC Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[10] non-coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/10 NC scar
sequence: https://synbiohub.org/public/igem/BBa_K1159996_sequence/1

25/10 NC scar
type: SequenceAnnotation
persistent identity: annotation2342869
display id: annotation2342869
version: 1
top level: BBa_K1159996
location: https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
type: Range
persistent identity: range2342869
display id: range2342869
version: 1
top level: BBa_K1159996
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_K1159106
type: ComponentDefinition
persistent identity: BBa K1159106
display id: BBa_K1159106
version: 1
was generated by: iGEM to SBOL conversion
description: PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This parts encodes are fusion protein consisting the photosensitive core of PhyB (2-908 AA) at the N-terminus and the C-terminal half of TEV Protease at its C-terminus. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions. Expressed with the second essential protein [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] or [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] containing the part which interacts with PhyB and the N-terminal half of the splitted TEV Protease, it form a light-switchable TEV Protease which is activated by red light and inactivated by far-red light. '''''Note: PhyB requires Phycocyanobilin or Phytochromobilin as a cofactor to fulfill its function as photoswitchable protein; you can reach it by adding exogenous Phycocyanobilin/Phytochromobilin in the medium or by inserting genes for the Phycocyanobilin/Phytochromobilin synthesis. See here for more information: [http://2012.igem.org/Team:TU_Munich/Project/Light_Switchable_Promoter#Biosynthesis_of_Phycocyanobilin] '''''
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K801031
component: BBa_K1159102
component: BBa_K1159993
component: BBa_K243029
sequence annotation: BBa_K801031
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159102
sequence: https://synbiohub.org/public/igem/BBa_K1159106_sequence/1

BBa_K801031
type: Component
persistent identity: component2346997
display id: component2346997
version: 1
top level: BBa_K1159106
definition: BBa_K801031
access: public

BBa_K1159102
type: Component
persistent identity: component2347006
display id: component2347006
version: 1
top level: BBa_K1159106
definition: BBa_K1159102
access: public

BBa_K1159993
type: Component
persistent identity: component2347003
display id: component2347003
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K243029
type: Component
persistent identity: component2347001
display id: component2347001
version: 1
top level: BBa_K1159106
definition: GSATLinker
access: public

BBa_K1159993
type: Component
persistent identity: component2346999
display id: component2346999
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2346997
display id: annotation2346997
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346997/range2346997/1
component: BBa_K801031

https://synbiohub.org/public/igem/BBa_K1159106/annotation2346997/range2346997/1
type: Range
persistent identity: range2346997
display id: range2346997
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 1
end: 2721
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346999
display id: annotation2346999
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346999/range2346999/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159106/annotation2346999/range2346999/1
type: Range
persistent identity: range2346999
display id: range2346999
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2722
end: 2727
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347001
display id: annotation2347001
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347001/range2347001/1
component: BBa_K243029

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347001/range2347001/1
type: Range
persistent identity: range2347001
display id: range2347001
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2728
end: 2835
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347003
display id: annotation2347003
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347003/range2347003/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347003/range2347003/1
type: Range
persistent identity: range2347003
display id: range2347003
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2836
end: 2841
orientation: inline

BBa_K1159102
type: SequenceAnnotation
persistent identity: annotation2347006
display id: annotation2347006
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347006/range2347006/1
component: BBa_K1159102

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347006/range2347006/1
type: Range
persistent identity: range2347006
display id: range2347006
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2842
end: 3195
orientation: inline

BBa_K1159107
type: ComponentDefinition
persistent identity: BBa K1159107
display id: BBa_K1159107
version: 1
was generated by: iGEM to SBOL conversion
description: N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This parts encodes a fusion protein consisting the N-terminal half of the TEV Protease as its terminus and first 100 amino acids of PIF3 as its C-terminus. PIF3 interacts with PhyB in the presence of red light. This reaction is reversible by far-red light. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions. By expressing this part together with the second essential protein [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] that contains the part, which interacts with PIF3, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K243029
component: BBa_K1159103
component: BBa_K1159101
sequence annotation: BBa_K1159101
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159103
sequence: https://synbiohub.org/public/igem/BBa_K1159107_sequence/1

BBa_K1159993
type: Component
persistent identity: component2347121
display id: component2347121
version: 1
top level: BBa_K1159107
definition: BBa_K1159993
access: public

BBa_K243029
type: Component
persistent identity: component2347119
display id: component2347119
version: 1
top level: BBa_K1159107
definition: GSATLinker
access: public

BBa_K1159103
type: Component
persistent identity: component2347123
display id: component2347123
version: 1
top level: BBa_K1159107
definition: BBa_K1159103
access: public

BBa_K1159993
type: Component
persistent identity: component2347117
display id: component2347117
version: 1
top level: BBa_K1159107
definition: BBa_K1159993
access: public

BBa_K1159101
type: Component
persistent identity: component2347115
display id: component2347115
version: 1
top level: BBa_K1159107
definition: BBa_K1159101
access: public

BBa_K1159101
type: SequenceAnnotation
persistent identity: annotation2347115
display id: annotation2347115
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347115/range2347115/1
component: BBa_K1159101

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347115/range2347115/1
type: Range
persistent identity: range2347115
display id: range2347115
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 1
end: 354
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347117
display id: annotation2347117
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347117/range2347117/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347117/range2347117/1
type: Range
persistent identity: range2347117
display id: range2347117
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 355
end: 360
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347119
display id: annotation2347119
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347119/range2347119/1
component: BBa_K243029

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347119/range2347119/1
type: Range
persistent identity: range2347119
display id: range2347119
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 361
end: 468
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347121
display id: annotation2347121
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347121/range2347121/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347121/range2347121/1
type: Range
persistent identity: range2347121
display id: range2347121
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 469
end: 474
orientation: inline

BBa_K1159103
type: SequenceAnnotation
persistent identity: annotation2347123
display id: annotation2347123
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347123/range2347123/1
component: BBa_K1159103

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347123/range2347123/1
type: Range
persistent identity: range2347123
display id: range2347123
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 475
end: 771
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159102
type: ComponentDefinition
persistent identity: BBa K1159102
display id: BBa_K1159102
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Tabacco Etch Virus
owned by: james
owned by: myers
mutable description: C-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary N-terminal half can be found under BBa_K1159101. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C-terminal half of TEV Protease
sequence annotation: Ser --> Val for autolysis resistance
sequence: https://synbiohub.org/public/igem/BBa_K1159102_sequence/1

C-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341474
display id: annotation2341474
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
type: Range
persistent identity: range2341474
display id: range2341474
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 1
end: 354
orientation: inline

Ser --> Val for autolysis resistance
type: SequenceAnnotation
persistent identity: annotation2341475
display id: annotation2341475
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
type: Range
persistent identity: range2341475
display id: range2341475
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 301
end: 303
orientation: inline

BBa_K801031
type: ComponentDefinition
persistent identity: BBa K801031
display id: BBa_K801031
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochrome B (PhyB, first 908 N-terminal amino acids)
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Based on BBa_K365002
owned by: james
owned by: myers
mutable description: improved version of BBa_K365002 to allow N- and C-terminal protein fusions using RFC 25
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: PhyB (2-908 AA) photosensitive core
sequence annotation: Essential Arg for photoconvertible binding of PIF3/6
sequence annotation: Essential Gly for photoconvertible binding of PIF3/6
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
sequence: https://synbiohub.org/public/igem/BBa_K801031_sequence/1

PhyB (2-908 AA) photosensitive core
type: SequenceAnnotation
persistent identity: annotation2343344
display id: annotation2343344
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
type: Range
persistent identity: range2343344
display id: range2343344
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1
end: 2721
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198856
display id: annotation2198856
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
type: Range
persistent identity: range2198856
display id: range2198856
version: 1
top level: BBa_K801031
direction: direction/forward
start: 325
end: 327
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198859
display id: annotation2198859
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
type: Range
persistent identity: range2198859
display id: range2198859
version: 1
top level: BBa_K801031
direction: direction/forward
start: 328
end: 330
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198861
display id: annotation2198861
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
type: Range
persistent identity: range2198861
display id: range2198861
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1039
end: 1041
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198862
display id: annotation2198862
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
type: Range
persistent identity: range2198862
display id: range2198862
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1051
end: 1053
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
type: SequenceAnnotation
persistent identity: annotation2198864
display id: annotation2198864
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
type: Range
persistent identity: range2198864
display id: range2198864
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1066
end: 1068
orientation: inline

GSATLinker
type: ComponentDefinition
persistent identity: BBa K243029
display id: BBa_K243029
version: 1
was generated by: iGEM to SBOL conversion
description: GSAT Linker
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Ordered by Mr.Gene.
owned by: james
owned by: myers
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The GSAT linker was create to connect our protein domains Fok_a and Fok_i to an function final construct.
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: GSAT Linker
sequence: https://synbiohub.org/public/igem/BBa_K243029_sequence/1

GSAT Linker
type: SequenceAnnotation
persistent identity: annotation2055435
display id: annotation2055435
version: 1
top level: GSATLinker
location: https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
type: Range
persistent identity: range2055435
display id: range2055435
version: 1
top level: GSATLinker
direction: direction/forward
start: 1
end: 108
orientation: inline

BBa_K1159103
type: ComponentDefinition
persistent identity: BBa K1159103
display id: BBa_K1159103
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal Phytochrome Interacting Factor 3 (PIF3) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Arabidopsis thaliana''
owned by: james
owned by: myers
mutable description: N-terminal Phytochrome Interacting Factor 3 (PIF3) is known to be recognized by Phytochrome B (PhyB) under red light exposition. This interaction between PhyB and PIF3 can be reverted by irradiation with far-red light. Only the first 100 amino acid residues of PIF3 is necessary for light-dependent binding to PhyB. N- and C-terminal protein fusions seems not to affect the binding of PhyB. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PIF3 (2-100 AA)
sequence: https://synbiohub.org/public/igem/BBa_K1159103_sequence/1

PIF3 (2-100 AA)
type: SequenceAnnotation
persistent identity: annotation2343342
display id: annotation2343342
version: 1
top level: BBa_K1159103
location: https://synbiohub.org/public/igem/BBa_K1159103/annotation2343342/range2343342/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159103/annotation2343342/range2343342/1
type: Range
persistent identity: range2343342
display id: range2343342
version: 1
top level: BBa_K1159103
direction: direction/forward
start: 1
end: 297
orientation: inline

BBa_K1159101
type: ComponentDefinition
persistent identity: BBa K1159101
display id: BBa_K1159101
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Tabacco Etch Virus''
owned by: james
owned by: myers
mutable description: N-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary C-terminal half can be found under BBa_K1159102. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: N-terminal half of TEV Protease
sequence: https://synbiohub.org/public/igem/BBa_K1159101_sequence/1

N-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341473
display id: annotation2341473
version: 1
top level: BBa_K1159101
location: https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
type: Range
persistent identity: range2341473
display id: range2341473
version: 1
top level: BBa_K1159101
direction: direction/forward
start: 1
end: 354
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159113_sequence/1
type: Sequence
persistent identity: BBa K1159113 sequence
display id: BBa_K1159113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159300_sequence/1
type: Sequence
persistent identity: BBa K1159300 sequence
display id: BBa_K1159300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159994_sequence/1
type: Sequence
persistent identity: BBa K1159994 sequence
display id: BBa_K1159994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159996_sequence/1
type: Sequence
persistent identity: BBa K1159996 sequence
display id: BBa_K1159996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159106_sequence/1
type: Sequence
persistent identity: BBa K1159106 sequence
display id: BBa_K1159106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159107_sequence/1
type: Sequence
persistent identity: BBa K1159107 sequence
display id: BBa_K1159107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactaccggcggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggcaccggccctctgtttgaacttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttcaagagctagagagattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159102_sequence/1
type: Sequence
persistent identity: BBa K1159102 sequence
display id: BBa_K1159102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagagcatgtctagcatggtgtcagacactagctgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatggtgaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801031_sequence/1
type: Sequence
persistent identity: BBa K801031 sequence
display id: BBa_K801031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243029_sequence/1
type: Sequence
persistent identity: BBa K243029 sequence
display id: BBa_K243029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159103_sequence/1
type: Sequence
persistent identity: BBa K1159103 sequence
display id: BBa_K1159103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctctgtttgaacttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttcaagagctagagagattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159101_sequence/1
type: Sequence
persistent identity: BBa K1159101 sequence
display id: BBa_K1159101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaact
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z