BBa_K1159117
type: ComponentDefinition
persistent identity: BBa K1159117
display id: BBa_K1159117
version: 1
was generated by: iGEM to SBOL conversion
description: Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K1159115
component: BBa_K1159111
component: BBa_K1159995
sequence annotation: BBa_K1159115
sequence annotation: BBa_K1159995
sequence annotation: BBa_K1159111
sequence: https://synbiohub.org/public/igem/BBa_K1159117_sequence/1

BBa_K1159115
type: Component
persistent identity: component2348221
display id: component2348221
version: 1
top level: BBa_K1159117
definition: BBa_K1159115
access: public

BBa_K1159111
type: Component
persistent identity: component2348247
display id: component2348247
version: 1
top level: BBa_K1159117
definition: BBa_K1159111
access: public

BBa_K1159995
type: Component
persistent identity: component2348223
display id: component2348223
version: 1
top level: BBa_K1159117
definition: BBa_K1159995
access: public

BBa_K1159115
type: SequenceAnnotation
persistent identity: annotation2348221
display id: annotation2348221
version: 1
top level: BBa_K1159117
location: https://synbiohub.org/public/igem/BBa_K1159117/annotation2348221/range2348221/1
component: BBa_K1159115

https://synbiohub.org/public/igem/BBa_K1159117/annotation2348221/range2348221/1
type: Range
persistent identity: range2348221
display id: range2348221
version: 1
top level: BBa_K1159117
direction: direction/reverse
start: 1
end: 5269
orientation: inline

BBa_K1159995
type: SequenceAnnotation
persistent identity: annotation2348223
display id: annotation2348223
version: 1
top level: BBa_K1159117
location: https://synbiohub.org/public/igem/BBa_K1159117/annotation2348223/range2348223/1
component: BBa_K1159995

https://synbiohub.org/public/igem/BBa_K1159117/annotation2348223/range2348223/1
type: Range
persistent identity: range2348223
display id: range2348223
version: 1
top level: BBa_K1159117
direction: direction/reverse
start: 5270
end: 5275
orientation: inline

BBa_K1159111
type: SequenceAnnotation
persistent identity: annotation2348247
display id: annotation2348247
version: 1
top level: BBa_K1159117
location: https://synbiohub.org/public/igem/BBa_K1159117/annotation2348247/range2348247/1
component: BBa_K1159111

https://synbiohub.org/public/igem/BBa_K1159117/annotation2348247/range2348247/1
type: Range
persistent identity: range2348247
display id: range2348247
version: 1
top level: BBa_K1159117
direction: direction/reverse
start: 5276
end: 6191
orientation: inline

BBa_K1159995
type: ComponentDefinition
persistent identity: BBa K1159995
display id: BBa_K1159995
version: 1
was generated by: iGEM to SBOL conversion
description: 10/25 N-Part Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] N-part Biobrick (not RFC[25] standard prefix!). Formally it has the same scar like the assembly scar between RFC[10] coding/non-coding Biobrick and RFC[10] coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 10/25 N-part scar
sequence: https://synbiohub.org/public/igem/BBa_K1159995_sequence/1

10/25 N-part scar
type: SequenceAnnotation
persistent identity: annotation2342867
display id: annotation2342867
version: 1
top level: BBa_K1159995
location: https://synbiohub.org/public/igem/BBa_K1159995/annotation2342867/range2342867/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159995/annotation2342867/range2342867/1
type: Range
persistent identity: range2342867
display id: range2342867
version: 1
top level: BBa_K1159995
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159111
type: ComponentDefinition
persistent identity: BBa K1159111
display id: BBa_K1159111
version: 1
was generated by: iGEM to SBOL conversion
description: Membrane-associated Thermonuclease NucA with TEV site and SV40 NLS in RFC[25] N-Part
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a membrane-associated Thermonuclease NucA for the chassis ''Physcomitrella patens''. NucA is a endo/exonuclease that degrades ssDNA/dsDNA/ssRNA/dsRNA. It cannot execute its deadly potential when it is bound to the cytoplasmatic membrane. The region between NucA and the transmembrane domain consists a long linker with a TEV cleavage site and a SV40 nuclear localization signal (NLS). By cleaving the TEV cleavage site with a TEV Protease the nuclease NucA translocates in the nucleus due to its SV40 NLS and degrades the genomic DNA resulting a inevitable induction of programmed cell-death (PCD). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159110
component: BBa_K1159993
component: BBa_K1159314
sequence annotation: BBa_K1159314
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159110
sequence: https://synbiohub.org/public/igem/BBa_K1159111_sequence/1

BBa_K1159110
type: Component
persistent identity: component2347358
display id: component2347358
version: 1
top level: BBa_K1159111
definition: BBa_K1159110
access: public

BBa_K1159993
type: Component
persistent identity: component2347345
display id: component2347345
version: 1
top level: BBa_K1159111
definition: BBa_K1159993
access: public

BBa_K1159314
type: Component
persistent identity: component2347343
display id: component2347343
version: 1
top level: BBa_K1159111
definition: BBa_K1159314
access: public

BBa_K1159314
type: SequenceAnnotation
persistent identity: annotation2347343
display id: annotation2347343
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347343/range2347343/1
component: BBa_K1159314

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347343/range2347343/1
type: Range
persistent identity: range2347343
display id: range2347343
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 1
end: 292
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347345
display id: annotation2347345
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347345/range2347345/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347345/range2347345/1
type: Range
persistent identity: range2347345
display id: range2347345
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 293
end: 298
orientation: inline

BBa_K1159110
type: SequenceAnnotation
persistent identity: annotation2347358
display id: annotation2347358
version: 1
top level: BBa_K1159111
location: https://synbiohub.org/public/igem/BBa_K1159111/annotation2347358/range2347358/1
component: BBa_K1159110

https://synbiohub.org/public/igem/BBa_K1159111/annotation2347358/range2347358/1
type: Range
persistent identity: range2347358
display id: range2347358
version: 1
top level: BBa_K1159111
direction: direction/reverse
start: 299
end: 916
orientation: inline

BBa_K1159115
type: ComponentDefinition
persistent identity: BBa K1159115
display id: BBa_K1159115
version: 1
was generated by: iGEM to SBOL conversion
description: Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based) with downstream IRES
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a light-switchable TEV Protease system consisting of two fusion proteins ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159107 BBa_K1159107]) seperated by an polioviral IRES [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159300 BBa_K1159300] which allows polycistronic expression in eukaryotic systems. You can easily express a third protein by simply clone your desired protein in the suffix of this part because this part ends with a polioviral IRES which allows cap-independent translation. After addition of your desired protein you can express this system by inserting your favored promoter/terminator in the prefix/suffix your construct. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159300
component: BBa_K1159996
component: BBa_K1159113
sequence annotation: BBa_K1159113
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence: https://synbiohub.org/public/igem/BBa_K1159115_sequence/1

BBa_K1159300
type: Component
persistent identity: component2347782
display id: component2347782
version: 1
top level: BBa_K1159115
definition: BBa_K1159300
access: public

BBa_K1159996
type: Component
persistent identity: component2347780
display id: component2347780
version: 1
top level: BBa_K1159115
definition: BBa_K1159996
access: public

BBa_K1159113
type: Component
persistent identity: component2347778
display id: component2347778
version: 1
top level: BBa_K1159115
definition: BBa_K1159113
access: public

BBa_K1159113
type: SequenceAnnotation
persistent identity: annotation2347778
display id: annotation2347778
version: 1
top level: BBa_K1159115
location: https://synbiohub.org/public/igem/BBa_K1159115/annotation2347778/range2347778/1
component: BBa_K1159113

https://synbiohub.org/public/igem/BBa_K1159115/annotation2347778/range2347778/1
type: Range
persistent identity: range2347778
display id: range2347778
version: 1
top level: BBa_K1159115
direction: direction/reverse
start: 1
end: 4625
orientation: inline

BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2347780
display id: annotation2347780
version: 1
top level: BBa_K1159115
location: https://synbiohub.org/public/igem/BBa_K1159115/annotation2347780/range2347780/1
component: BBa_K1159996

https://synbiohub.org/public/igem/BBa_K1159115/annotation2347780/range2347780/1
type: Range
persistent identity: range2347780
display id: range2347780
version: 1
top level: BBa_K1159115
direction: direction/reverse
start: 4626
end: 4642
orientation: inline

BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2347782
display id: annotation2347782
version: 1
top level: BBa_K1159115
location: https://synbiohub.org/public/igem/BBa_K1159115/annotation2347782/range2347782/1
component: BBa_K1159300

https://synbiohub.org/public/igem/BBa_K1159115/annotation2347782/range2347782/1
type: Range
persistent identity: range2347782
display id: range2347782
version: 1
top level: BBa_K1159115
direction: direction/reverse
start: 4643
end: 5269
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159300
type: ComponentDefinition
persistent identity: BBa K1159300
display id: BBa_K1159300
version: 1
was generated by: iGEM to SBOL conversion
description: Polioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotes
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:55:50Z
mutable provenance: Human Poliovirus 1 Mahoney Strain
owned by: james
owned by: myers
mutable description: This part contains the Internal Ribosome Entry Site (IRES) of the human poliovirus 1 Mahoney strain which allows polycistronic expression systems in eukaryotic systems. This part is flanked by RFC[10] pre- and suffix. By cloning this part betweens CDSs, many proteins/peptides can expressed by a single promoter and terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Polioviral IRES
sequence: https://synbiohub.org/public/igem/BBa_K1159300_sequence/1

Polioviral IRES
type: SequenceAnnotation
persistent identity: annotation2343585
display id: annotation2343585
version: 1
top level: BBa_K1159300
location: https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
type: Range
persistent identity: range2343585
display id: range2343585
version: 1
top level: BBa_K1159300
direction: direction/forward
start: 1
end: 627
orientation: inline

BBa_K1159996
type: ComponentDefinition
persistent identity: BBa K1159996
display id: BBa_K1159996
version: 1
was generated by: iGEM to SBOL conversion
description: 25/10 NC Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[10] non-coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/10 NC scar
sequence: https://synbiohub.org/public/igem/BBa_K1159996_sequence/1

25/10 NC scar
type: SequenceAnnotation
persistent identity: annotation2342869
display id: annotation2342869
version: 1
top level: BBa_K1159996
location: https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
type: Range
persistent identity: range2342869
display id: range2342869
version: 1
top level: BBa_K1159996
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_K1159110
type: ComponentDefinition
persistent identity: BBa K1159110
display id: BBa_K1159110
version: 1
was generated by: iGEM to SBOL conversion
description: Thermonuclease with N-terminal SV40 NLS and flexible linker with TEV site in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS) and a flexible linker with a TEV cleavage site. Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159109
component: BBa_K1159993
component: BBa_K1159310
sequence annotation: BBa_K1159310
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159109
sequence: https://synbiohub.org/public/igem/BBa_K1159110_sequence/1

BBa_K1159109
type: Component
persistent identity: component2347172
display id: component2347172
version: 1
top level: BBa_K1159110
definition: BBa_K1159109
access: public

BBa_K1159993
type: Component
persistent identity: component2347165
display id: component2347165
version: 1
top level: BBa_K1159110
definition: BBa_K1159993
access: public

BBa_K1159310
type: Component
persistent identity: component2347163
display id: component2347163
version: 1
top level: BBa_K1159110
definition: BBa_K1159310
access: public

BBa_K1159310
type: SequenceAnnotation
persistent identity: annotation2347163
display id: annotation2347163
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347163/range2347163/1
component: BBa_K1159310

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347163/range2347163/1
type: Range
persistent identity: range2347163
display id: range2347163
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 1
end: 102
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347165
display id: annotation2347165
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347165/range2347165/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347165/range2347165/1
type: Range
persistent identity: range2347165
display id: range2347165
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 103
end: 108
orientation: inline

BBa_K1159109
type: SequenceAnnotation
persistent identity: annotation2347172
display id: annotation2347172
version: 1
top level: BBa_K1159110
location: https://synbiohub.org/public/igem/BBa_K1159110/annotation2347172/range2347172/1
component: BBa_K1159109

https://synbiohub.org/public/igem/BBa_K1159110/annotation2347172/range2347172/1
type: Range
persistent identity: range2347172
display id: range2347172
version: 1
top level: BBa_K1159110
direction: direction/reverse
start: 109
end: 618
orientation: inline

BBa_K1159314
type: ComponentDefinition
persistent identity: BBa K1159314
display id: BBa_K1159314
version: 1
was generated by: iGEM to SBOL conversion
description: TMD with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25] N-Part
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T01:53:32Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Transmembrane Domain with N-terminal Signal Peptide of SERK Receptor from ''P. patens'' in RFC[25]. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible.
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K1159305
component: BBa_K1159993
component: BBa_K1159303
sequence annotation: BBa_K1159303
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159305
sequence: https://synbiohub.org/public/igem/BBa_K1159314_sequence/1

BBa_K1159305
type: Component
persistent identity: component2347285
display id: component2347285
version: 1
top level: BBa_K1159314
definition: BBa_K1159305
access: public

BBa_K1159993
type: Component
persistent identity: component2347283
display id: component2347283
version: 1
top level: BBa_K1159314
definition: BBa_K1159993
access: public

BBa_K1159303
type: Component
persistent identity: component2347281
display id: component2347281
version: 1
top level: BBa_K1159314
definition: BBa_K1159303
access: public

BBa_K1159303
type: SequenceAnnotation
persistent identity: annotation2347281
display id: annotation2347281
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
component: BBa_K1159303

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347281/range2347281/1
type: Range
persistent identity: range2347281
display id: range2347281
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347283
display id: annotation2347283
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347283/range2347283/1
type: Range
persistent identity: range2347283
display id: range2347283
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 101
end: 106
orientation: inline

BBa_K1159305
type: SequenceAnnotation
persistent identity: annotation2347285
display id: annotation2347285
version: 1
top level: BBa_K1159314
location: https://synbiohub.org/public/igem/BBa_K1159314/annotation2347285/range2347285/1
component: BBa_K1159305

https://synbiohub.org/public/igem/BBa_K1159314/annotation2347285/range2347285/1
type: Range
persistent identity: range2347285
display id: range2347285
version: 1
top level: BBa_K1159314
direction: direction/reverse
start: 107
end: 292
orientation: inline

BBa_K1159113
type: ComponentDefinition
persistent identity: BBa K1159113
display id: BBa_K1159113
version: 1
was generated by: iGEM to SBOL conversion
description: Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF3 based)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a light-switchable TEV Protease system consisting of two fusion proteins ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159107 BBa_K1159107]) seperated by an polioviral IRES [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159300 BBa_K1159300] which allows polycistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159996
component: BBa_K1159106
component: BBa_K1159107
component: BBa_K1159994
component: BBa_K1159300
sequence annotation: BBa_K1159106
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence annotation: BBa_K1159994
sequence annotation: BBa_K1159107
sequence: https://synbiohub.org/public/igem/BBa_K1159113_sequence/1

BBa_K1159996
type: Component
persistent identity: component2347553
display id: component2347553
version: 1
top level: BBa_K1159113
definition: BBa_K1159996
access: public

BBa_K1159106
type: Component
persistent identity: component2347551
display id: component2347551
version: 1
top level: BBa_K1159113
definition: BBa_K1159106
access: public

BBa_K1159107
type: Component
persistent identity: component2347568
display id: component2347568
version: 1
top level: BBa_K1159113
definition: BBa_K1159107
access: public

BBa_K1159994
type: Component
persistent identity: component2347557
display id: component2347557
version: 1
top level: BBa_K1159113
definition: BBa_K1159994
access: public

BBa_K1159300
type: Component
persistent identity: component2347555
display id: component2347555
version: 1
top level: BBa_K1159113
definition: BBa_K1159300
access: public

BBa_K1159106
type: SequenceAnnotation
persistent identity: annotation2347551
display id: annotation2347551
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347551/range2347551/1
component: BBa_K1159106

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347551/range2347551/1
type: Range
persistent identity: range2347551
display id: range2347551
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 1
end: 3195
orientation: inline

BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2347553
display id: annotation2347553
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347553/range2347553/1
component: BBa_K1159996

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347553/range2347553/1
type: Range
persistent identity: range2347553
display id: range2347553
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3196
end: 3212
orientation: inline

BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2347555
display id: annotation2347555
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347555/range2347555/1
component: BBa_K1159300

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347555/range2347555/1
type: Range
persistent identity: range2347555
display id: range2347555
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3213
end: 3839
orientation: inline

BBa_K1159994
type: SequenceAnnotation
persistent identity: annotation2347557
display id: annotation2347557
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347557/range2347557/1
component: BBa_K1159994

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347557/range2347557/1
type: Range
persistent identity: range2347557
display id: range2347557
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3840
end: 3854
orientation: inline

BBa_K1159107
type: SequenceAnnotation
persistent identity: annotation2347568
display id: annotation2347568
version: 1
top level: BBa_K1159113
location: https://synbiohub.org/public/igem/BBa_K1159113/annotation2347568/range2347568/1
component: BBa_K1159107

https://synbiohub.org/public/igem/BBa_K1159113/annotation2347568/range2347568/1
type: Range
persistent identity: range2347568
display id: range2347568
version: 1
top level: BBa_K1159113
direction: direction/reverse
start: 3855
end: 4625
orientation: inline

BBa_K1159303
type: ComponentDefinition
persistent identity: BBa K1159303
display id: BBa_K1159303
version: 1
was generated by: iGEM to SBOL conversion
description: Signal Peptide of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:49Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: Consensus Sequence for ''P. patens''
sequence annotation: SERK Signal Peptide
sequence: https://synbiohub.org/public/igem/BBa_K1159303_sequence/1

Consensus Sequence for ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2341512
display id: annotation2341512
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341512/range2341512/1
type: Range
persistent identity: range2341512
display id: range2341512
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 1
end: 4
orientation: inline

SERK Signal Peptide
type: SequenceAnnotation
persistent identity: annotation2341513
display id: annotation2341513
version: 1
top level: BBa_K1159303
location: https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159303/annotation2341513/range2341513/1
type: Range
persistent identity: range2341513
display id: range2341513
version: 1
top level: BBa_K1159303
direction: direction/forward
start: 5
end: 100
orientation: inline

BBa_K1159305
type: ComponentDefinition
persistent identity: BBa K1159305
display id: BBa_K1159305
version: 1
was generated by: iGEM to SBOL conversion
description: Transmembrane domain of SERK Receptor from Physcomitrella patens in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:54:11Z
mutable provenance: ''Physcomitrella patens''
owned by: james
owned by: myers
mutable description: This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: Transmembrane Domain of SERK Receptor from ''P. patens''
sequence: https://synbiohub.org/public/igem/BBa_K1159305_sequence/1

Transmembrane Domain of SERK Receptor from ''P. patens''
type: SequenceAnnotation
persistent identity: annotation2343588
display id: annotation2343588
version: 1
top level: BBa_K1159305
location: https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159305/annotation2343588/range2343588/1
type: Range
persistent identity: range2343588
display id: range2343588
version: 1
top level: BBa_K1159305
direction: direction/forward
start: 1
end: 186
orientation: inline

BBa_K1159310
type: ComponentDefinition
persistent identity: BBa K1159310
display id: BBa_K1159310
version: 1
was generated by: iGEM to SBOL conversion
description: 34 AA flexible linker with C-terminal TEV cleavage site (GGGGS)x5-TEV-site-linker) in RFC[25]
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a 34 amino acids long flexible linker ((GlyGlyGlyGlySer)x5) with a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000417
role: partType/Protein Domain
sequence annotation: (GlyGlyGlySer)x5
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1159310_sequence/1

(GlyGlyGlySer)x5
type: SequenceAnnotation
persistent identity: annotation2342593
display id: annotation2342593
version: 1
top level: BBa_K1159310
location: https://synbiohub.org/public/igem/BBa_K1159310/annotation2342593/range2342593/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159310/annotation2342593/range2342593/1
type: Range
persistent identity: range2342593
display id: range2342593
version: 1
top level: BBa_K1159310
direction: direction/forward
start: 1
end: 75
orientation: inline

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2342592
display id: annotation2342592
version: 1
top level: BBa_K1159310
location: https://synbiohub.org/public/igem/BBa_K1159310/annotation2342592/range2342592/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159310/annotation2342592/range2342592/1
type: Range
persistent identity: range2342592
display id: range2342592
version: 1
top level: BBa_K1159310
direction: direction/forward
start: 79
end: 99
orientation: inline

BBa_K1159994
type: ComponentDefinition
persistent identity: BBa K1159994
display id: BBa_K1159994
version: 1
was generated by: iGEM to SBOL conversion
description: 10/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] Biobrick (not RFC[25] N-part!).
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: 10/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159994_sequence/1

10/25 scar
type: SequenceAnnotation
persistent identity: annotation2342865
display id: annotation2342865
version: 1
top level: BBa_K1159994
location: https://synbiohub.org/public/igem/BBa_K1159994/annotation2342865/range2342865/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159994/annotation2342865/range2342865/1
type: Range
persistent identity: range2342865
display id: range2342865
version: 1
top level: BBa_K1159994
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K1159106
type: ComponentDefinition
persistent identity: BBa K1159106
display id: BBa_K1159106
version: 1
was generated by: iGEM to SBOL conversion
description: PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This parts encodes are fusion protein consisting the photosensitive core of PhyB (2-908 AA) at the N-terminus and the C-terminal half of TEV Protease at its C-terminus. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions. Expressed with the second essential protein [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] or [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] containing the part which interacts with PhyB and the N-terminal half of the splitted TEV Protease, it form a light-switchable TEV Protease which is activated by red light and inactivated by far-red light. '''''Note: PhyB requires Phycocyanobilin or Phytochromobilin as a cofactor to fulfill its function as photoswitchable protein; you can reach it by adding exogenous Phycocyanobilin/Phytochromobilin in the medium or by inserting genes for the Phycocyanobilin/Phytochromobilin synthesis. See here for more information: [http://2012.igem.org/Team:TU_Munich/Project/Light_Switchable_Promoter#Biosynthesis_of_Phycocyanobilin] '''''
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K801031
component: BBa_K1159102
component: BBa_K1159993
component: BBa_K243029
sequence annotation: BBa_K801031
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159102
sequence: https://synbiohub.org/public/igem/BBa_K1159106_sequence/1

BBa_K801031
type: Component
persistent identity: component2346997
display id: component2346997
version: 1
top level: BBa_K1159106
definition: BBa_K801031
access: public

BBa_K1159102
type: Component
persistent identity: component2347006
display id: component2347006
version: 1
top level: BBa_K1159106
definition: BBa_K1159102
access: public

BBa_K1159993
type: Component
persistent identity: component2347003
display id: component2347003
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K243029
type: Component
persistent identity: component2347001
display id: component2347001
version: 1
top level: BBa_K1159106
definition: GSATLinker
access: public

BBa_K1159993
type: Component
persistent identity: component2346999
display id: component2346999
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2346997
display id: annotation2346997
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346997/range2346997/1
component: BBa_K801031

https://synbiohub.org/public/igem/BBa_K1159106/annotation2346997/range2346997/1
type: Range
persistent identity: range2346997
display id: range2346997
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 1
end: 2721
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346999
display id: annotation2346999
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346999/range2346999/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159106/annotation2346999/range2346999/1
type: Range
persistent identity: range2346999
display id: range2346999
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2722
end: 2727
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347001
display id: annotation2347001
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347001/range2347001/1
component: BBa_K243029

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347001/range2347001/1
type: Range
persistent identity: range2347001
display id: range2347001
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2728
end: 2835
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347003
display id: annotation2347003
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347003/range2347003/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347003/range2347003/1
type: Range
persistent identity: range2347003
display id: range2347003
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2836
end: 2841
orientation: inline

BBa_K1159102
type: SequenceAnnotation
persistent identity: annotation2347006
display id: annotation2347006
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347006/range2347006/1
component: BBa_K1159102

https://synbiohub.org/public/igem/BBa_K1159106/annotation2347006/range2347006/1
type: Range
persistent identity: range2347006
display id: range2347006
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2842
end: 3195
orientation: inline

BBa_K1159107
type: ComponentDefinition
persistent identity: BBa K1159107
display id: BBa_K1159107
version: 1
was generated by: iGEM to SBOL conversion
description: N-TEV-Protease_36AALinker_PIF3 (Second part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This parts encodes a fusion protein consisting the N-terminal half of the TEV Protease as its terminus and first 100 amino acids of PIF3 as its C-terminus. PIF3 interacts with PhyB in the presence of red light. This reaction is reversible by far-red light. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions. By expressing this part together with the second essential protein [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] that contains the part, which interacts with PIF3, and the C-terminal half of the splitted TEV Protease, they form together a light-switchable TEV Protease system which can be activated by red light and inactivated by far-red light.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159993
component: BBa_K243029
component: BBa_K1159103
component: BBa_K1159101
sequence annotation: BBa_K1159101
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159103
sequence: https://synbiohub.org/public/igem/BBa_K1159107_sequence/1

BBa_K1159993
type: Component
persistent identity: component2347121
display id: component2347121
version: 1
top level: BBa_K1159107
definition: BBa_K1159993
access: public

BBa_K243029
type: Component
persistent identity: component2347119
display id: component2347119
version: 1
top level: BBa_K1159107
definition: GSATLinker
access: public

BBa_K1159103
type: Component
persistent identity: component2347123
display id: component2347123
version: 1
top level: BBa_K1159107
definition: BBa_K1159103
access: public

BBa_K1159993
type: Component
persistent identity: component2347117
display id: component2347117
version: 1
top level: BBa_K1159107
definition: BBa_K1159993
access: public

BBa_K1159101
type: Component
persistent identity: component2347115
display id: component2347115
version: 1
top level: BBa_K1159107
definition: BBa_K1159101
access: public

BBa_K1159101
type: SequenceAnnotation
persistent identity: annotation2347115
display id: annotation2347115
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347115/range2347115/1
component: BBa_K1159101

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347115/range2347115/1
type: Range
persistent identity: range2347115
display id: range2347115
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 1
end: 354
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347117
display id: annotation2347117
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347117/range2347117/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347117/range2347117/1
type: Range
persistent identity: range2347117
display id: range2347117
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 355
end: 360
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347119
display id: annotation2347119
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347119/range2347119/1
component: BBa_K243029

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347119/range2347119/1
type: Range
persistent identity: range2347119
display id: range2347119
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 361
end: 468
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347121
display id: annotation2347121
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347121/range2347121/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347121/range2347121/1
type: Range
persistent identity: range2347121
display id: range2347121
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 469
end: 474
orientation: inline

BBa_K1159103
type: SequenceAnnotation
persistent identity: annotation2347123
display id: annotation2347123
version: 1
top level: BBa_K1159107
location: https://synbiohub.org/public/igem/BBa_K1159107/annotation2347123/range2347123/1
component: BBa_K1159103

https://synbiohub.org/public/igem/BBa_K1159107/annotation2347123/range2347123/1
type: Range
persistent identity: range2347123
display id: range2347123
version: 1
top level: BBa_K1159107
direction: direction/reverse
start: 475
end: 771
orientation: inline

BBa_K1159109
type: ComponentDefinition
persistent identity: BBa K1159109
display id: BBa_K1159109
version: 1
was generated by: iGEM to SBOL conversion
description: Micrococcal nuclease (Thermonuclease) with N-terminal SV40 NLS in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a micrococcal nuclease (Thermonuclease) with N-terminal SV40 Nuclear Localization Signal (SV40 NLS). Thermonuclease degrades ssDNA/dsDNA/ssRNA/dsRNA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159105
component: BBa_K1159993
component: BBa_K801030
sequence annotation: BBa_K801030
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159105
sequence: https://synbiohub.org/public/igem/BBa_K1159109_sequence/1

BBa_K1159105
type: Component
persistent identity: component2347160
display id: component2347160
version: 1
top level: BBa_K1159109
definition: BBa_K1159105
access: public

BBa_K1159993
type: Component
persistent identity: component2347158
display id: component2347158
version: 1
top level: BBa_K1159109
definition: BBa_K1159993
access: public

BBa_K801030
type: Component
persistent identity: component2347156
display id: component2347156
version: 1
top level: BBa_K1159109
definition: BBa_K801030
access: public

BBa_K801030
type: SequenceAnnotation
persistent identity: annotation2347156
display id: annotation2347156
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347156/range2347156/1
component: BBa_K801030

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347156/range2347156/1
type: Range
persistent identity: range2347156
display id: range2347156
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347158
display id: annotation2347158
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347158/range2347158/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347158/range2347158/1
type: Range
persistent identity: range2347158
display id: range2347158
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 58
end: 63
orientation: inline

BBa_K1159105
type: SequenceAnnotation
persistent identity: annotation2347160
display id: annotation2347160
version: 1
top level: BBa_K1159109
location: https://synbiohub.org/public/igem/BBa_K1159109/annotation2347160/range2347160/1
component: BBa_K1159105

https://synbiohub.org/public/igem/BBa_K1159109/annotation2347160/range2347160/1
type: Range
persistent identity: range2347160
display id: range2347160
version: 1
top level: BBa_K1159109
direction: direction/reverse
start: 64
end: 510
orientation: inline

BBa_K1159102
type: ComponentDefinition
persistent identity: BBa K1159102
display id: BBa_K1159102
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Tabacco Etch Virus
owned by: james
owned by: myers
mutable description: C-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary N-terminal half can be found under BBa_K1159101. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C-terminal half of TEV Protease
sequence annotation: Ser --> Val for autolysis resistance
sequence: https://synbiohub.org/public/igem/BBa_K1159102_sequence/1

C-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341474
display id: annotation2341474
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
type: Range
persistent identity: range2341474
display id: range2341474
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 1
end: 354
orientation: inline

Ser --> Val for autolysis resistance
type: SequenceAnnotation
persistent identity: annotation2341475
display id: annotation2341475
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
type: Range
persistent identity: range2341475
display id: range2341475
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 301
end: 303
orientation: inline

BBa_K801031
type: ComponentDefinition
persistent identity: BBa K801031
display id: BBa_K801031
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochrome B (PhyB, first 908 N-terminal amino acids)
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Based on BBa_K365002
owned by: james
owned by: myers
mutable description: improved version of BBa_K365002 to allow N- and C-terminal protein fusions using RFC 25
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: PhyB (2-908 AA) photosensitive core
sequence annotation: Essential Arg for photoconvertible binding of PIF3/6
sequence annotation: Essential Gly for photoconvertible binding of PIF3/6
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
sequence: https://synbiohub.org/public/igem/BBa_K801031_sequence/1

PhyB (2-908 AA) photosensitive core
type: SequenceAnnotation
persistent identity: annotation2343344
display id: annotation2343344
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
type: Range
persistent identity: range2343344
display id: range2343344
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1
end: 2721
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198856
display id: annotation2198856
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
type: Range
persistent identity: range2198856
display id: range2198856
version: 1
top level: BBa_K801031
direction: direction/forward
start: 325
end: 327
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198859
display id: annotation2198859
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
type: Range
persistent identity: range2198859
display id: range2198859
version: 1
top level: BBa_K801031
direction: direction/forward
start: 328
end: 330
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198861
display id: annotation2198861
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
type: Range
persistent identity: range2198861
display id: range2198861
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1039
end: 1041
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198862
display id: annotation2198862
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
type: Range
persistent identity: range2198862
display id: range2198862
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1051
end: 1053
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
type: SequenceAnnotation
persistent identity: annotation2198864
display id: annotation2198864
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
type: Range
persistent identity: range2198864
display id: range2198864
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1066
end: 1068
orientation: inline

GSATLinker
type: ComponentDefinition
persistent identity: BBa K243029
display id: BBa_K243029
version: 1
was generated by: iGEM to SBOL conversion
description: GSAT Linker
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Ordered by Mr.Gene.
owned by: james
owned by: myers
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The GSAT linker was create to connect our protein domains Fok_a and Fok_i to an function final construct.
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: GSAT Linker
sequence: https://synbiohub.org/public/igem/BBa_K243029_sequence/1

GSAT Linker
type: SequenceAnnotation
persistent identity: annotation2055435
display id: annotation2055435
version: 1
top level: GSATLinker
location: https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
type: Range
persistent identity: range2055435
display id: range2055435
version: 1
top level: GSATLinker
direction: direction/forward
start: 1
end: 108
orientation: inline

BBa_K1159101
type: ComponentDefinition
persistent identity: BBa K1159101
display id: BBa_K1159101
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Tabacco Etch Virus''
owned by: james
owned by: myers
mutable description: N-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary C-terminal half can be found under BBa_K1159102. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: N-terminal half of TEV Protease
sequence: https://synbiohub.org/public/igem/BBa_K1159101_sequence/1

N-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341473
display id: annotation2341473
version: 1
top level: BBa_K1159101
location: https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
type: Range
persistent identity: range2341473
display id: range2341473
version: 1
top level: BBa_K1159101
direction: direction/forward
start: 1
end: 354
orientation: inline

BBa_K1159103
type: ComponentDefinition
persistent identity: BBa K1159103
display id: BBa_K1159103
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal Phytochrome Interacting Factor 3 (PIF3) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Arabidopsis thaliana''
owned by: james
owned by: myers
mutable description: N-terminal Phytochrome Interacting Factor 3 (PIF3) is known to be recognized by Phytochrome B (PhyB) under red light exposition. This interaction between PhyB and PIF3 can be reverted by irradiation with far-red light. Only the first 100 amino acid residues of PIF3 is necessary for light-dependent binding to PhyB. N- and C-terminal protein fusions seems not to affect the binding of PhyB. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PIF3 (2-100 AA)
sequence: https://synbiohub.org/public/igem/BBa_K1159103_sequence/1

PIF3 (2-100 AA)
type: SequenceAnnotation
persistent identity: annotation2343342
display id: annotation2343342
version: 1
top level: BBa_K1159103
location: https://synbiohub.org/public/igem/BBa_K1159103/annotation2343342/range2343342/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159103/annotation2343342/range2343342/1
type: Range
persistent identity: range2343342
display id: range2343342
version: 1
top level: BBa_K1159103
direction: direction/forward
start: 1
end: 297
orientation: inline

BBa_K1159105
type: ComponentDefinition
persistent identity: BBa K1159105
display id: BBa_K1159105
version: 1
was generated by: iGEM to SBOL conversion
description: Mature Nuclease NucA from Staphylococcus aureus (Thermonuclease) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Staphylococcus aureus''
owned by: james
owned by: myers
mutable description: Thermonuclease is a endo-exonuclease from ''Staphylococcus aureus'' that degrades dsDNA, ssDNA, dsRNA and ssRNA. This part is coding the mature form of the nuclease, also called NucA. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Mature Thermonuclease (NucA)
sequence: https://synbiohub.org/public/igem/BBa_K1159105_sequence/1

Mature Thermonuclease (NucA)
type: SequenceAnnotation
persistent identity: annotation2343335
display id: annotation2343335
version: 1
top level: BBa_K1159105
location: https://synbiohub.org/public/igem/BBa_K1159105/annotation2343335/range2343335/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1159105/annotation2343335/range2343335/1
type: Range
persistent identity: range2343335
display id: range2343335
version: 1
top level: BBa_K1159105
direction: direction/forward
start: 1
end: 447
orientation: inline

BBa_K801030
type: ComponentDefinition
persistent identity: BBa K801030
display id: BBa_K801030
version: 1
was generated by: iGEM to SBOL conversion
description: Polypeptide containing SV40 nuclear localization sequence (SV40 NLS) for nuclear translocation
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: designed by oligo hybridisation based on plasmid "pGADT7 AD" from Clontech, w/ RFC25-pre and suffix for protein fusions
owned by: james
owned by: myers
mutable description: SV40NLS nuclear localization sequence
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: SV40 nuclear localization sequence (recognized by importin alpha)
sequence: https://synbiohub.org/public/igem/BBa_K801030_sequence/1

SV40 nuclear localization sequence (recognized by importin alpha)
type: SequenceAnnotation
persistent identity: annotation2198843
display id: annotation2198843
version: 1
top level: BBa_K801030
location: https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K801030/annotation2198843/range2198843/1
type: Range
persistent identity: range2198843
display id: range2198843
version: 1
top level: BBa_K801030
direction: direction/forward
start: 22
end: 42
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159117_sequence/1
type: Sequence
persistent identity: BBa K1159117 sequence
display id: BBa_K1159117_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159995_sequence/1
type: Sequence
persistent identity: BBa K1159995 sequence
display id: BBa_K1159995_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159111_sequence/1
type: Sequence
persistent identity: BBa K1159111 sequence
display id: BBa_K1159111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159115_sequence/1
type: Sequence
persistent identity: BBa K1159115 sequence
display id: BBa_K1159115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159300_sequence/1
type: Sequence
persistent identity: BBa K1159300 sequence
display id: BBa_K1159300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159996_sequence/1
type: Sequence
persistent identity: BBa K1159996 sequence
display id: BBa_K1159996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159110_sequence/1
type: Sequence
persistent identity: BBa K1159110 sequence
display id: BBa_K1159110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcaggaaccggcgcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159314_sequence/1
type: Sequence
persistent identity: BBa K1159314 sequence
display id: BBa_K1159314_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159113_sequence/1
type: Sequence
persistent identity: BBa K1159113 sequence
display id: BBa_K1159113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159303_sequence/1
type: Sequence
persistent identity: BBa K1159303 sequence
display id: BBa_K1159303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159305_sequence/1
type: Sequence
persistent identity: BBa K1159305 sequence
display id: BBa_K1159305_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159310_sequence/1
type: Sequence
persistent identity: BBa K1159310 sequence
display id: BBa_K1159310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggaggcggttcaggcggaggtggctctggcggtggcggatcgggaggcggtggctccggaggtggaggctcaggagagaatttgtattttcagtcagga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159994_sequence/1
type: Sequence
persistent identity: BBa K1159994 sequence
display id: BBa_K1159994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159106_sequence/1
type: Sequence
persistent identity: BBa K1159106 sequence
display id: BBa_K1159106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159107_sequence/1
type: Sequence
persistent identity: BBa K1159107 sequence
display id: BBa_K1159107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactaccggcggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggcaccggccctctgtttgaacttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttcaagagctagagagattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159109_sequence/1
type: Sequence
persistent identity: BBa K1159109 sequence
display id: BBa_K1159109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgccaccggcgcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159102_sequence/1
type: Sequence
persistent identity: BBa K1159102 sequence
display id: BBa_K1159102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagagcatgtctagcatggtgtcagacactagctgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatggtgaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801031_sequence/1
type: Sequence
persistent identity: BBa K801031 sequence
display id: BBa_K801031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243029_sequence/1
type: Sequence
persistent identity: BBa K243029 sequence
display id: BBa_K243029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159101_sequence/1
type: Sequence
persistent identity: BBa K1159101 sequence
display id: BBa_K1159101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159103_sequence/1
type: Sequence
persistent identity: BBa K1159103 sequence
display id: BBa_K1159103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctctgtttgaacttttcaggctcaccaaagctaagcttgaatctgctcaagacaggaacccttctccacctgtagatgaagttgtggagctggtgtgggaaaatggtcagatatcaactcaaagtcagtcaagtagatcgaggaacattcctccaccacaagcaaactcttcaagagctagagagattggaaatggctcaaagacgactatggtggacgagatccctatgtcagtgccatcactaatgacgggtttgagtcaagacgatgactttgttccatggttgaatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159105_sequence/1
type: Sequence
persistent identity: BBa K1159105 sequence
display id: BBa_K1159105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaacttcaactaaaaaattacataaagaacctgcgactttaattaaagcgattgatggtgatacggttaaattaatgtacaaaggtcaaccaatgacattcagactattattggttgatacacctgaaacaaagcatcctaaaaaaggtgtagagaaatatggtcctgaagcaagtgcatttacgaaaaaaatggtagaaaatgcaaagaaaattgaagtcgagtttgacaaaggtcaaagaactgataaatatggacgtggcttagcgtatatttatgctgatggaaaaatggtaaacgaagctttagttcgtcaaggcttggctaaagttgcttatgtttacaaacctaacaatacacatgaacaacatttaagaaaaagtgaagcacaagcgaaaaaagagaaattaaatatttggagcgaagacaacgctgattcaggtcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801030_sequence/1
type: Sequence
persistent identity: BBa K801030 sequence
display id: BBa_K801030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcggaattaattcccgagcctccaaaaaagaagagaaaggtcgaattgggtaccgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z