##gff-version 3
##sequence-region BBa_K1159120 1 6751
BBa_K1159120	.	engineered_region	1	5269	.	+	0	ID=BBa_K1159116;Name=BBa_K1159116
BBa_K1159120	.	engineered_region	1	4625	.	+	0	ID=BBa_K1159114;Name=BBa_K1159114;Parent=BBa_K1159116
BBa_K1159120	.	CDS	3855	4625	.	+	0	ID=BBa_K1159108;Name=BBa_K1159108;Parent=BBa_K1159114
BBa_K1159120	.	sequence_feature	4323	4328	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159108
BBa_K1159120	.	sequence_feature	4323	4328	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	CDS	4329	4625	.	+	0	ID=BBa_K1159104;Name=BBa_K1159104;Parent=BBa_K1159108
BBa_K1159120	.	CDS	4329	4625	.	+	0	ID=annotation2343343;Name=PIF6 (2-100 AA);Parent=BBa_K1159104
BBa_K1159120	.	polypeptide_domain	4215	4322	.	+	0	ID=BBa_K243029;Name=BBa_K243029;Parent=BBa_K1159108
BBa_K1159120	.	sequence_feature	4215	4322	.	+	0	ID=annotation2055435;Name=GSAT Linker;Parent=BBa_K243029
BBa_K1159120	.	CDS	3855	4208	.	+	0	ID=BBa_K1159101;Name=BBa_K1159101;Parent=BBa_K1159108
BBa_K1159120	.	CDS	3855	4208	.	+	0	ID=annotation2341473;Name=N-terminal half of TEV Protease;Parent=BBa_K1159101
BBa_K1159120	.	sequence_feature	4209	4214	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159108
BBa_K1159120	.	sequence_feature	4209	4214	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	CDS	1	3195	.	+	0	ID=BBa_K1159106;Name=BBa_K1159106;Parent=BBa_K1159114
BBa_K1159120	.	polypeptide_domain	1	2721	.	+	0	ID=BBa_K801031;Name=BBa_K801031;Parent=BBa_K1159106
BBa_K1159120	.	conserved_region	328	330	.	+	0	ID=annotation2198859;Name=Essential Gly for photoconvertible binding of PIF3/6;Parent=BBa_K801031
BBa_K1159120	.	conserved_region	1039	1041	.	+	0	ID=annotation2198861;Name=Essential Gly for photoconvertible binding of PIF3/6;Parent=BBa_K801031
BBa_K1159120	.	conserved_region	1066	1068	.	+	0	ID=annotation2198864;Name=Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin;Parent=BBa_K801031
BBa_K1159120	.	CDS	1	2721	.	+	0	ID=annotation2343344;Name=PhyB (2-908 AA) photosensitive core;Parent=BBa_K801031
BBa_K1159120	.	conserved_region	325	327	.	+	0	ID=annotation2198856;Name=Essential Arg for photoconvertible binding of PIF3/6;Parent=BBa_K801031
BBa_K1159120	.	conserved_region	1051	1053	.	+	0	ID=annotation2198862;Name=Essential Arg for photoconvertible binding of PIF3/6;Parent=BBa_K801031
BBa_K1159120	.	polypeptide_domain	2728	2835	.	+	0	ID=BBa_K243029;Name=BBa_K243029;Parent=BBa_K1159106
BBa_K1159120	.	sequence_feature	2728	2835	.	+	0	ID=annotation2055435;Name=GSAT Linker;Parent=BBa_K243029
BBa_K1159120	.	sequence_feature	2722	2727	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159106
BBa_K1159120	.	sequence_feature	2722	2727	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	CDS	2842	3195	.	+	0	ID=BBa_K1159102;Name=BBa_K1159102;Parent=BBa_K1159106
BBa_K1159120	.	sequence_alteration	3142	3144	.	+	0	ID=annotation2341475;Name=Ser --> Val for autolysis resistance;Parent=BBa_K1159102
BBa_K1159120	.	CDS	2842	3195	.	+	0	ID=annotation2341474;Name=C-terminal half of TEV Protease;Parent=BBa_K1159102
BBa_K1159120	.	sequence_feature	2836	2841	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159106
BBa_K1159120	.	sequence_feature	2836	2841	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	sequence_feature	3196	3212	.	+	0	ID=BBa_K1159996;Name=BBa_K1159996;Parent=BBa_K1159114
BBa_K1159120	.	sequence_feature	3196	3212	.	+	0	ID=annotation2342869;Name=25/10 NC scar;Parent=BBa_K1159996
BBa_K1159120	.	ribosome_entry_site	3213	3839	.	+	0	ID=BBa_K1159300;Name=BBa_K1159300;Parent=BBa_K1159114
BBa_K1159120	.	ribosome_entry_site	3213	3839	.	+	0	ID=annotation2343585;Name=Polioviral IRES;Parent=BBa_K1159300
BBa_K1159120	.	sequence_feature	3840	3854	.	+	0	ID=BBa_K1159994;Name=BBa_K1159994;Parent=BBa_K1159114
BBa_K1159120	.	sequence_feature	3840	3854	.	+	0	ID=annotation2342865;Name=10/25 scar;Parent=BBa_K1159994
BBa_K1159120	.	sequence_feature	4626	4642	.	+	0	ID=BBa_K1159996;Name=BBa_K1159996;Parent=BBa_K1159116
BBa_K1159120	.	sequence_feature	4626	4642	.	+	0	ID=annotation2342869;Name=25/10 NC scar;Parent=BBa_K1159996
BBa_K1159120	.	ribosome_entry_site	4643	5269	.	+	0	ID=BBa_K1159300;Name=BBa_K1159300;Parent=BBa_K1159116
BBa_K1159120	.	ribosome_entry_site	4643	5269	.	+	0	ID=annotation2343585;Name=Polioviral IRES;Parent=BBa_K1159300
BBa_K1159120	.	sequence_feature	5270	5284	.	+	0	ID=BBa_K1159994;Name=BBa_K1159994
BBa_K1159120	.	sequence_feature	5270	5284	.	+	0	ID=annotation2342865;Name=10/25 scar;Parent=BBa_K1159994
BBa_K1159120	.	CDS	5285	6751	.	+	0	ID=BBa_K1159112;Name=BBa_K1159112
BBa_K1159120	.	sequence_feature	6032	6037	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159112
BBa_K1159120	.	sequence_feature	6032	6037	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	CDS	5285	5998	.	+	0	ID=BBa_K1159302;Name=BBa_K1159302;Parent=BBa_K1159112
BBa_K1159120	.	CDS	5285	5998	.	+	0	ID=annotation2343589;Name=eCFP;Parent=BBa_K1159302
BBa_K1159120	.	CDS	6038	6751	.	+	0	ID=BBa_K1159301;Name=BBa_K1159301;Parent=BBa_K1159112
BBa_K1159120	.	CDS	6038	6751	.	+	0	ID=annotation2343586;Name=SYFP2;Parent=BBa_K1159301
BBa_K1159120	.	sequence_feature	5999	6004	.	+	0	ID=BBa_K1159993;Name=BBa_K1159993;Parent=BBa_K1159112
BBa_K1159120	.	sequence_feature	5999	6004	.	+	0	ID=annotation2342863;Name=25/25 scar;Parent=BBa_K1159993
BBa_K1159120	.	CDS	6005	6031	.	+	0	ID=BBa_K1159318;Name=BBa_K1159318;Parent=BBa_K1159112
BBa_K1159120	.	sequence_feature	6008	6028	.	+	0	ID=annotation2347399;Name=TEV cleavage site;Parent=BBa_K1159318
>BBa_K1159120
gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactc
ctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaat
gagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatca
ttcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctc
gaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggtta
cagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatg
ggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattacct
tgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgt
tgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcg
gttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtga
gggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaa
acgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaag
cagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtcta
tgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtc
tttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctt
tggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgc
aggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagac
actgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaa
tgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatg
ttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccgg
tgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcga
tctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatc
ctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgat
tcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtg
gttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctca
ttgagactgcaactgttcctatattcgctgtggatgccggaggctgcatcaatggatggaacgctaagattgcagagtt
gacaggtctctcagttgaagaagctatggggaagtctctggtttctgatttaatatacaaagagaatgaagcaactgtc
aataagcttctttctcgtgctttgagaggggacgaggaaaagaatgtggaggttaagctgaaaactttcagccccgaac
tacaagggaaagcagtttttgtggttgtgaatgcttgttccagcaaggactacttgaacaacattgtcggcgtttgttt
tgttggacaagacgttacgagtcagaaaatcgtaatggataagttcatcaacatacaaggagattacaaggctattgta
catagcccaaaccctctaatcccgccaatttttgctgctgacgagaacacgtgctgcctggaatggaacatggcgatgg
aaaagcttacgggttggtctcgcagtgaagtgattgggaaaatgattgtcggggaagtgtttgggagctgttgcatgct
aaagggtcctgatgctttaaccaagttcatgattgtattgcataatgcgattggtggccaagatacggataagttccct
ttcccattctttgaccgcaatgggaagtttgttcaggctctattgactgcaaacaagcgggttagcctcgagggaaagg
ttattggggctttctgtttcttgcaaatcccgagcaccggcggtggttctgccggtggctccggttctggctccagcgg
tggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggcaccggcaag
agcatgtctagcatggtgtcagacactagctgcacattcccttcatctgatggcatattctggaagcattggattcaaa
ccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaattt
caccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgg
gttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatggtgaaacctgaagagcctt
ttcagccagttaaggaagcgactcaactcatgaataccggttaatactagagttaaaacagctctggggttgtacccac
cccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaact
tagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtga
tgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacct
cggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccgg
tgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagag
cctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtg
attggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggc
tgcttatggtgacaatcacagattgttatcataaagcgaattggatttactagatggccggcggagaaagcttgtttaa
ggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttg
tatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaat
cactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattat
tcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtctt
gtgacaaccaacttccaaactaccggcggtggttctgccggtggctccggttctggctccagcggtggcagctctggtg
cgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggcaccggcatgttcttaccaaccga
ttattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaaga
tccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatga
agagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgc
tgcccatgaaacaaacatgttggaaagcaataaacatgttgacaccggttaatactagagttaaaacagctctggggtt
gtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttc
ccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttc
cccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccag
taccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatcc
ctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggt
gtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcaca
aaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttatttt
attgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatttactagatggccggcgtgagcaag
ggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgt
ccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgcc
ctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgac
ttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagaccc
gcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaa
catcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatc
aaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccccca
tcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaa
gcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaccggc
ggagagaatttgtattttcagtcaggaaccggcgttagcaagggcgaagaactttttacaggcgtagtaccgatcttag
ttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagct
gaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgtt
cagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcagg
agcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaa
tcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcc
cacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacg
gtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccatta
tctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgct
gccggtattaccctgggtatggatgagctgtataaa