BBa_K1159120
type: ComponentDefinition
persistent identity: BBa K1159120
display id: BBa_K1159120
version: 1
was generated by: iGEM to SBOL conversion
description: Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)
created: 2013-09-15T11:00:00Z
modified: 2015-06-11T02:00:45Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Red light triggered TEV Protease system with FRET Reporter for plants translation unit (PhyB/PIF6 version)
discontinued: false
dominant: false
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159112
component: BBa_K1159994
component: BBa_K1159116
sequence annotation: BBa_K1159116
sequence annotation: BBa_K1159994
sequence annotation: BBa_K1159112
sequence: https://synbiohub.org/public/igem/BBa_K1159120_sequence/1

BBa_K1159112
type: Component
persistent identity: component2348473
display id: component2348473
version: 1
top level: BBa_K1159120
definition: BBa_K1159112
access: public

BBa_K1159994
type: Component
persistent identity: component2348462
display id: component2348462
version: 1
top level: BBa_K1159120
definition: BBa_K1159994
access: public

BBa_K1159116
type: Component
persistent identity: component2348460
display id: component2348460
version: 1
top level: BBa_K1159120
definition: BBa_K1159116
access: public

BBa_K1159116
type: SequenceAnnotation
persistent identity: annotation2348460
display id: annotation2348460
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348460/range2348460/1
component: BBa_K1159116

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persistent identity: range2348460
display id: range2348460
version: 1
top level: BBa_K1159120
direction: direction/reverse
start: 1
end: 5269
orientation: inline

BBa_K1159994
type: SequenceAnnotation
persistent identity: annotation2348462
display id: annotation2348462
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348462/range2348462/1
component: BBa_K1159994

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type: Range
persistent identity: range2348462
display id: range2348462
version: 1
top level: BBa_K1159120
direction: direction/reverse
start: 5270
end: 5284
orientation: inline

BBa_K1159112
type: SequenceAnnotation
persistent identity: annotation2348473
display id: annotation2348473
version: 1
top level: BBa_K1159120
location: https://synbiohub.org/public/igem/BBa_K1159120/annotation2348473/range2348473/1
component: BBa_K1159112

https://synbiohub.org/public/igem/BBa_K1159120/annotation2348473/range2348473/1
type: Range
persistent identity: range2348473
display id: range2348473
version: 1
top level: BBa_K1159120
direction: direction/reverse
start: 5285
end: 6751
orientation: inline

BBa_K1159994
type: ComponentDefinition
persistent identity: BBa K1159994
display id: BBa_K1159994
version: 1
was generated by: iGEM to SBOL conversion
description: 10/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[10] coding/non-coding Biobrick and RFC[25] Biobrick (not RFC[25] N-part!).
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 10/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159994_sequence/1

10/25 scar
type: SequenceAnnotation
persistent identity: annotation2342865
display id: annotation2342865
version: 1
top level: BBa_K1159994
location: https://synbiohub.org/public/igem/BBa_K1159994/annotation2342865/range2342865/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2342865
display id: range2342865
version: 1
top level: BBa_K1159994
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K1159112
type: ComponentDefinition
persistent identity: BBa K1159112
display id: BBa_K1159112
version: 1
was generated by: iGEM to SBOL conversion
description: FRET Reporter for TEV Protease activity (eCFP_TEV-site-linker_SYFP2) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a FRET reporter with the chromophores eCFP and SYFP2 that is seperated by a TEV cleavage site. As a FRET pair, excitation of eCFP leads to a energy transfer from eCFP to SYFP2 (FRET) resulting in a fluorescence of SYFP2. In the presence of TEV Protease cleavage actitivy the FRET pair SYFP2 is cleaved off from eCFP and eCFP is not anymore quenched by SYFP2 resulting in the fluorescence of eCFP. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159301
component: BBa_K1159993
component: BBa_K1159318
component: BBa_K1159302
sequence annotation: BBa_K1159302
sequence annotation: BBa_K1159993
sequence annotation: BBa_K1159318
sequence annotation: BBa_K1159301
sequence: https://synbiohub.org/public/igem/BBa_K1159112_sequence/1

BBa_K1159301
type: Component
persistent identity: component2347450
display id: component2347450
version: 1
top level: BBa_K1159112
definition: BBa_K1159301
access: public

BBa_K1159993
type: Component
persistent identity: component2347448
display id: component2347448
version: 1
top level: BBa_K1159112
definition: BBa_K1159993
access: public

BBa_K1159318
type: Component
persistent identity: component2347446
display id: component2347446
version: 1
top level: BBa_K1159112
definition: BBa_K1159318
access: public

BBa_K1159993
type: Component
persistent identity: component2347444
display id: component2347444
version: 1
top level: BBa_K1159112
definition: BBa_K1159993
access: public

BBa_K1159302
type: Component
persistent identity: component2347442
display id: component2347442
version: 1
top level: BBa_K1159112
definition: BBa_K1159302
access: public

BBa_K1159302
type: SequenceAnnotation
persistent identity: annotation2347442
display id: annotation2347442
version: 1
top level: BBa_K1159112
location: https://synbiohub.org/public/igem/BBa_K1159112/annotation2347442/range2347442/1
component: BBa_K1159302

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type: Range
persistent identity: range2347442
display id: range2347442
version: 1
top level: BBa_K1159112
direction: direction/reverse
start: 1
end: 714
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347444
display id: annotation2347444
version: 1
top level: BBa_K1159112
location: https://synbiohub.org/public/igem/BBa_K1159112/annotation2347444/range2347444/1
component: BBa_K1159993

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type: Range
persistent identity: range2347444
display id: range2347444
version: 1
top level: BBa_K1159112
direction: direction/reverse
start: 715
end: 720
orientation: inline

BBa_K1159318
type: SequenceAnnotation
persistent identity: annotation2347446
display id: annotation2347446
version: 1
top level: BBa_K1159112
location: https://synbiohub.org/public/igem/BBa_K1159112/annotation2347446/range2347446/1
component: BBa_K1159318

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type: Range
persistent identity: range2347446
display id: range2347446
version: 1
top level: BBa_K1159112
direction: direction/reverse
start: 721
end: 747
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347448
display id: annotation2347448
version: 1
top level: BBa_K1159112
location: https://synbiohub.org/public/igem/BBa_K1159112/annotation2347448/range2347448/1
component: BBa_K1159993

https://synbiohub.org/public/igem/BBa_K1159112/annotation2347448/range2347448/1
type: Range
persistent identity: range2347448
display id: range2347448
version: 1
top level: BBa_K1159112
direction: direction/reverse
start: 748
end: 753
orientation: inline

BBa_K1159301
type: SequenceAnnotation
persistent identity: annotation2347450
display id: annotation2347450
version: 1
top level: BBa_K1159112
location: https://synbiohub.org/public/igem/BBa_K1159112/annotation2347450/range2347450/1
component: BBa_K1159301

https://synbiohub.org/public/igem/BBa_K1159112/annotation2347450/range2347450/1
type: Range
persistent identity: range2347450
display id: range2347450
version: 1
top level: BBa_K1159112
direction: direction/reverse
start: 754
end: 1467
orientation: inline

BBa_K1159116
type: ComponentDefinition
persistent identity: BBa K1159116
display id: BBa_K1159116
version: 1
was generated by: iGEM to SBOL conversion
description: Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based) with downstream IRES
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a light-switchable TEV Protease system consisting of two fusion proteins ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159108 BBa_K1159108]) seperated by an polioviral IRES [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159300 BBa_K1159300] which allows polycistronic expression in eukaryotic systems. You can easily express a third protein by simply clone your desired protein in the suffix of this part because this part ends with a polioviral IRES which allows cap-independent translation. After addition of your desired protein you can express this system by inserting your favored promoter/terminator in the prefix/suffix your construct. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159114
component: BBa_K1159300
component: BBa_K1159996
sequence annotation: BBa_K1159114
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence: https://synbiohub.org/public/igem/BBa_K1159116_sequence/1

BBa_K1159114
type: Component
persistent identity: component2347823
display id: component2347823
version: 1
top level: BBa_K1159116
definition: BBa_K1159114
access: public

BBa_K1159300
type: Component
persistent identity: component2347827
display id: component2347827
version: 1
top level: BBa_K1159116
definition: BBa_K1159300
access: public

BBa_K1159996
type: Component
persistent identity: component2347825
display id: component2347825
version: 1
top level: BBa_K1159116
definition: BBa_K1159996
access: public

BBa_K1159114
type: SequenceAnnotation
persistent identity: annotation2347823
display id: annotation2347823
version: 1
top level: BBa_K1159116
location: https://synbiohub.org/public/igem/BBa_K1159116/annotation2347823/range2347823/1
component: BBa_K1159114

https://synbiohub.org/public/igem/BBa_K1159116/annotation2347823/range2347823/1
type: Range
persistent identity: range2347823
display id: range2347823
version: 1
top level: BBa_K1159116
direction: direction/reverse
start: 1
end: 4625
orientation: inline

BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2347825
display id: annotation2347825
version: 1
top level: BBa_K1159116
location: https://synbiohub.org/public/igem/BBa_K1159116/annotation2347825/range2347825/1
component: BBa_K1159996

https://synbiohub.org/public/igem/BBa_K1159116/annotation2347825/range2347825/1
type: Range
persistent identity: range2347825
display id: range2347825
version: 1
top level: BBa_K1159116
direction: direction/reverse
start: 4626
end: 4642
orientation: inline

BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2347827
display id: annotation2347827
version: 1
top level: BBa_K1159116
location: https://synbiohub.org/public/igem/BBa_K1159116/annotation2347827/range2347827/1
component: BBa_K1159300

https://synbiohub.org/public/igem/BBa_K1159116/annotation2347827/range2347827/1
type: Range
persistent identity: range2347827
display id: range2347827
version: 1
top level: BBa_K1159116
direction: direction/reverse
start: 4643
end: 5269
orientation: inline

BBa_K1159993
type: ComponentDefinition
persistent identity: BBa K1159993
display id: BBa_K1159993
version: 1
was generated by: iGEM to SBOL conversion
description: 25/25 Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/25 scar
sequence: https://synbiohub.org/public/igem/BBa_K1159993_sequence/1

25/25 scar
type: SequenceAnnotation
persistent identity: annotation2342863
display id: annotation2342863
version: 1
top level: BBa_K1159993
location: https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1159993/annotation2342863/range2342863/1
type: Range
persistent identity: range2342863
display id: range2342863
version: 1
top level: BBa_K1159993
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_K1159300
type: ComponentDefinition
persistent identity: BBa K1159300
display id: BBa_K1159300
version: 1
was generated by: iGEM to SBOL conversion
description: Polioviral Internal Ribosome Entry Site (IRES) for polycistronic expression in eukaryotes
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:55:50Z
mutable provenance: Human Poliovirus 1 Mahoney Strain
owned by: james
owned by: myers
mutable description: This part contains the Internal Ribosome Entry Site (IRES) of the human poliovirus 1 Mahoney strain which allows polycistronic expression systems in eukaryotic systems. This part is flanked by RFC[10] pre- and suffix. By cloning this part betweens CDSs, many proteins/peptides can expressed by a single promoter and terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Polioviral IRES
sequence: https://synbiohub.org/public/igem/BBa_K1159300_sequence/1

Polioviral IRES
type: SequenceAnnotation
persistent identity: annotation2343585
display id: annotation2343585
version: 1
top level: BBa_K1159300
location: https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1159300/annotation2343585/range2343585/1
type: Range
persistent identity: range2343585
display id: range2343585
version: 1
top level: BBa_K1159300
direction: direction/forward
start: 1
end: 627
orientation: inline

BBa_K1159301
type: ComponentDefinition
persistent identity: BBa K1159301
display id: BBa_K1159301
version: 1
was generated by: iGEM to SBOL conversion
description: Super Yellow Fluorescent Protein 2 (SYFP2) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2016-01-26T02:17:14Z
mutable provenance: ''Aequorea victoria''
owned by: james
owned by: myers
mutable description: Improved part of BBa_K864100 which originally cannot be fused to other protein domains. This improved version is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: SYFP2
sequence: https://synbiohub.org/public/igem/BBa_K1159301_sequence/1

SYFP2
type: SequenceAnnotation
persistent identity: annotation2343586
display id: annotation2343586
version: 1
top level: BBa_K1159301
location: https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159301/annotation2343586/range2343586/1
type: Range
persistent identity: range2343586
display id: range2343586
version: 1
top level: BBa_K1159301
direction: direction/forward
start: 1
end: 714
orientation: inline

BBa_K1159302
type: ComponentDefinition
persistent identity: BBa K1159302
display id: BBa_K1159302
version: 1
was generated by: iGEM to SBOL conversion
description: Enhanced Cyan Fluorescent Protein (eCFP) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2016-01-26T03:03:36Z
mutable provenance: ''Aequorea victoria''
owned by: james
owned by: myers
mutable description: This Biobricks is a improved version BBa_E0020 which codes for the Enhanced Cyan Fluorescent Protein. We improve the function of the biobrick, to allow protein fusions which is originally not possible due to RFC[10] pre- and suffix. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: eCFP
sequence: https://synbiohub.org/public/igem/BBa_K1159302_sequence/1

eCFP
type: SequenceAnnotation
persistent identity: annotation2343589
display id: annotation2343589
version: 1
top level: BBa_K1159302
location: https://synbiohub.org/public/igem/BBa_K1159302/annotation2343589/range2343589/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1159302/annotation2343589/range2343589/1
type: Range
persistent identity: range2343589
display id: range2343589
version: 1
top level: BBa_K1159302
direction: direction/forward
start: 1
end: 714
orientation: inline

BBa_K1159996
type: ComponentDefinition
persistent identity: BBa K1159996
display id: BBa_K1159996
version: 1
was generated by: iGEM to SBOL conversion
description: 25/10 NC Assembly Scar
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: Assembly scar between RFC[25] Biobrick and RFC[10] non-coding Biobrick.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: 25/10 NC scar
sequence: https://synbiohub.org/public/igem/BBa_K1159996_sequence/1

25/10 NC scar
type: SequenceAnnotation
persistent identity: annotation2342869
display id: annotation2342869
version: 1
top level: BBa_K1159996
location: https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159996/annotation2342869/range2342869/1
type: Range
persistent identity: range2342869
display id: range2342869
version: 1
top level: BBa_K1159996
direction: direction/forward
start: 1
end: 17
orientation: inline

BBa_K1159318
type: ComponentDefinition
persistent identity: BBa K1159318
display id: BBa_K1159318
version: 1
was generated by: iGEM to SBOL conversion
description: TEV cleavage site flanked by Gly for better accessibility in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes the TEV cleavage site Glu-Asn-Leu-Tyr-Phe-Gln-Ser (ENLYFQS) flanked by Gly for better accessibility in RFC[25].
discontinued: false
dominant: false
experience: experience/Works
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: TEV cleavage site
sequence: https://synbiohub.org/public/igem/BBa_K1159318_sequence/1

TEV cleavage site
type: SequenceAnnotation
persistent identity: annotation2347399
display id: annotation2347399
version: 1
top level: BBa_K1159318
location: https://synbiohub.org/public/igem/BBa_K1159318/annotation2347399/range2347399/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1159318/annotation2347399/range2347399/1
type: Range
persistent identity: range2347399
display id: range2347399
version: 1
top level: BBa_K1159318
direction: direction/forward
start: 4
end: 24
orientation: inline

BBa_K1159114
type: ComponentDefinition
persistent identity: BBa K1159114
display id: BBa_K1159114
version: 1
was generated by: iGEM to SBOL conversion
description: Eukaryotic light-switchable TEV Protease translational unit (PhyB/PIF6 based)
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part encodes a light-switchable TEV Protease system consisting of two fusion proteins ([http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159106 BBa_K1159106] and [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159108 BBa_K1159108]) seperated by an polioviral IRES [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159300 BBa_K1159300] which allows polycistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K1159108
component: BBa_K1159994
component: BBa_K1159300
component: BBa_K1159996
component: BBa_K1159106
sequence annotation: BBa_K1159106
sequence annotation: BBa_K1159996
sequence annotation: BBa_K1159300
sequence annotation: BBa_K1159994
sequence annotation: BBa_K1159108
sequence: https://synbiohub.org/public/igem/BBa_K1159114_sequence/1

BBa_K1159108
type: Component
persistent identity: component2347610
display id: component2347610
version: 1
top level: BBa_K1159114
definition: BBa_K1159108
access: public

BBa_K1159994
type: Component
persistent identity: component2347599
display id: component2347599
version: 1
top level: BBa_K1159114
definition: BBa_K1159994
access: public

BBa_K1159300
type: Component
persistent identity: component2347597
display id: component2347597
version: 1
top level: BBa_K1159114
definition: BBa_K1159300
access: public

BBa_K1159996
type: Component
persistent identity: component2347595
display id: component2347595
version: 1
top level: BBa_K1159114
definition: BBa_K1159996
access: public

BBa_K1159106
type: Component
persistent identity: component2347593
display id: component2347593
version: 1
top level: BBa_K1159114
definition: BBa_K1159106
access: public

BBa_K1159106
type: SequenceAnnotation
persistent identity: annotation2347593
display id: annotation2347593
version: 1
top level: BBa_K1159114
location: https://synbiohub.org/public/igem/BBa_K1159114/annotation2347593/range2347593/1
component: BBa_K1159106

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BBa_K1159996
type: SequenceAnnotation
persistent identity: annotation2347595
display id: annotation2347595
version: 1
top level: BBa_K1159114
location: https://synbiohub.org/public/igem/BBa_K1159114/annotation2347595/range2347595/1
component: BBa_K1159996

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persistent identity: range2347595
display id: range2347595
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BBa_K1159300
type: SequenceAnnotation
persistent identity: annotation2347597
display id: annotation2347597
version: 1
top level: BBa_K1159114
location: https://synbiohub.org/public/igem/BBa_K1159114/annotation2347597/range2347597/1
component: BBa_K1159300

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persistent identity: range2347597
display id: range2347597
version: 1
top level: BBa_K1159114
direction: direction/reverse
start: 3213
end: 3839
orientation: inline

BBa_K1159994
type: SequenceAnnotation
persistent identity: annotation2347599
display id: annotation2347599
version: 1
top level: BBa_K1159114
location: https://synbiohub.org/public/igem/BBa_K1159114/annotation2347599/range2347599/1
component: BBa_K1159994

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persistent identity: range2347599
display id: range2347599
version: 1
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start: 3840
end: 3854
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BBa_K1159108
type: SequenceAnnotation
persistent identity: annotation2347610
display id: annotation2347610
version: 1
top level: BBa_K1159114
location: https://synbiohub.org/public/igem/BBa_K1159114/annotation2347610/range2347610/1
component: BBa_K1159108

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persistent identity: range2347610
display id: range2347610
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top level: BBa_K1159114
direction: direction/reverse
start: 3855
end: 4625
orientation: inline

BBa_K1159106
type: ComponentDefinition
persistent identity: BBa K1159106
display id: BBa_K1159106
version: 1
was generated by: iGEM to SBOL conversion
description: PhyB(2-908 AA)_36AALinker_C-TEV-Protease (First part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This parts encodes are fusion protein consisting the photosensitive core of PhyB (2-908 AA) at the N-terminus and the C-terminal half of TEV Protease at its C-terminus. Both parts are seperated by a 36 amino acids long flexible linker. This part is flanked by RFC[25] pre- and suffix to allow further protein fusions. Expressed with the second essential protein [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] or [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159315] containing the part which interacts with PhyB and the N-terminal half of the splitted TEV Protease, it form a light-switchable TEV Protease which is activated by red light and inactivated by far-red light. '''''Note: PhyB requires Phycocyanobilin or Phytochromobilin as a cofactor to fulfill its function as photoswitchable protein; you can reach it by adding exogenous Phycocyanobilin/Phytochromobilin in the medium or by inserting genes for the Phycocyanobilin/Phytochromobilin synthesis. See here for more information: [http://2012.igem.org/Team:TU_Munich/Project/Light_Switchable_Promoter#Biosynthesis_of_Phycocyanobilin] '''''
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
component: BBa_K801031
component: BBa_K1159102
component: BBa_K1159993
component: BBa_K243029
sequence annotation: BBa_K801031
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159102
sequence: https://synbiohub.org/public/igem/BBa_K1159106_sequence/1

BBa_K801031
type: Component
persistent identity: component2346997
display id: component2346997
version: 1
top level: BBa_K1159106
definition: BBa_K801031
access: public

BBa_K1159102
type: Component
persistent identity: component2347006
display id: component2347006
version: 1
top level: BBa_K1159106
definition: BBa_K1159102
access: public

BBa_K1159993
type: Component
persistent identity: component2347003
display id: component2347003
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K243029
type: Component
persistent identity: component2347001
display id: component2347001
version: 1
top level: BBa_K1159106
definition: GSATLinker
access: public

BBa_K1159993
type: Component
persistent identity: component2346999
display id: component2346999
version: 1
top level: BBa_K1159106
definition: BBa_K1159993
access: public

BBa_K801031
type: SequenceAnnotation
persistent identity: annotation2346997
display id: annotation2346997
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346997/range2346997/1
component: BBa_K801031

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type: Range
persistent identity: range2346997
display id: range2346997
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 1
end: 2721
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2346999
display id: annotation2346999
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2346999/range2346999/1
component: BBa_K1159993

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type: Range
persistent identity: range2346999
display id: range2346999
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2722
end: 2727
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347001
display id: annotation2347001
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347001/range2347001/1
component: BBa_K243029

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type: Range
persistent identity: range2347001
display id: range2347001
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2728
end: 2835
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347003
display id: annotation2347003
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347003/range2347003/1
component: BBa_K1159993

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type: Range
persistent identity: range2347003
display id: range2347003
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2836
end: 2841
orientation: inline

BBa_K1159102
type: SequenceAnnotation
persistent identity: annotation2347006
display id: annotation2347006
version: 1
top level: BBa_K1159106
location: https://synbiohub.org/public/igem/BBa_K1159106/annotation2347006/range2347006/1
component: BBa_K1159102

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type: Range
persistent identity: range2347006
display id: range2347006
version: 1
top level: BBa_K1159106
direction: direction/reverse
start: 2842
end: 3195
orientation: inline

BBa_K1159108
type: ComponentDefinition
persistent identity: BBa K1159108
display id: BBa_K1159108
version: 1
was generated by: iGEM to SBOL conversion
description: N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Synthetic
owned by: myers
owned by: james
mutable description: N-TEV-Protease_36AALinker_PIF6 (Second part for a light-switchable TEV-Protease) in RFC[25]
discontinued: false
dominant: false
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_K1159101
component: BBa_K243029
component: BBa_K1159993
component: BBa_K1159104
sequence annotation: BBa_K1159101
sequence annotation: BBa_K1159993
sequence annotation: BBa_K243029
sequence annotation: BBa_K1159104
sequence: https://synbiohub.org/public/igem/BBa_K1159108_sequence/1

BBa_K1159101
type: Component
persistent identity: component2347101
display id: component2347101
version: 1
top level: BBa_K1159108
definition: BBa_K1159101
access: public

BBa_K243029
type: Component
persistent identity: component2347105
display id: component2347105
version: 1
top level: BBa_K1159108
definition: GSATLinker
access: public

BBa_K1159993
type: Component
persistent identity: component2347103
display id: component2347103
version: 1
top level: BBa_K1159108
definition: BBa_K1159993
access: public

BBa_K1159104
type: Component
persistent identity: component2347109
display id: component2347109
version: 1
top level: BBa_K1159108
definition: BBa_K1159104
access: public

BBa_K1159993
type: Component
persistent identity: component2347107
display id: component2347107
version: 1
top level: BBa_K1159108
definition: BBa_K1159993
access: public

BBa_K1159101
type: SequenceAnnotation
persistent identity: annotation2347101
display id: annotation2347101
version: 1
top level: BBa_K1159108
location: https://synbiohub.org/public/igem/BBa_K1159108/annotation2347101/range2347101/1
component: BBa_K1159101

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type: Range
persistent identity: range2347101
display id: range2347101
version: 1
top level: BBa_K1159108
direction: direction/reverse
start: 1
end: 354
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347103
display id: annotation2347103
version: 1
top level: BBa_K1159108
location: https://synbiohub.org/public/igem/BBa_K1159108/annotation2347103/range2347103/1
component: BBa_K1159993

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type: Range
persistent identity: range2347103
display id: range2347103
version: 1
top level: BBa_K1159108
direction: direction/reverse
start: 355
end: 360
orientation: inline

BBa_K243029
type: SequenceAnnotation
persistent identity: annotation2347105
display id: annotation2347105
version: 1
top level: BBa_K1159108
location: https://synbiohub.org/public/igem/BBa_K1159108/annotation2347105/range2347105/1
component: BBa_K243029

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type: Range
persistent identity: range2347105
display id: range2347105
version: 1
top level: BBa_K1159108
direction: direction/reverse
start: 361
end: 468
orientation: inline

BBa_K1159993
type: SequenceAnnotation
persistent identity: annotation2347107
display id: annotation2347107
version: 1
top level: BBa_K1159108
location: https://synbiohub.org/public/igem/BBa_K1159108/annotation2347107/range2347107/1
component: BBa_K1159993

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type: Range
persistent identity: range2347107
display id: range2347107
version: 1
top level: BBa_K1159108
direction: direction/reverse
start: 469
end: 474
orientation: inline

BBa_K1159104
type: SequenceAnnotation
persistent identity: annotation2347109
display id: annotation2347109
version: 1
top level: BBa_K1159108
location: https://synbiohub.org/public/igem/BBa_K1159108/annotation2347109/range2347109/1
component: BBa_K1159104

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type: Range
persistent identity: range2347109
display id: range2347109
version: 1
top level: BBa_K1159108
direction: direction/reverse
start: 475
end: 771
orientation: inline

BBa_K1159101
type: ComponentDefinition
persistent identity: BBa K1159101
display id: BBa_K1159101
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Tabacco Etch Virus''
owned by: james
owned by: myers
mutable description: N-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary C-terminal half can be found under BBa_K1159102. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: N-terminal half of TEV Protease
sequence: https://synbiohub.org/public/igem/BBa_K1159101_sequence/1

N-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341473
display id: annotation2341473
version: 1
top level: BBa_K1159101
location: https://synbiohub.org/public/igem/BBa_K1159101/annotation2341473/range2341473/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range2341473
display id: range2341473
version: 1
top level: BBa_K1159101
direction: direction/forward
start: 1
end: 354
orientation: inline

BBa_K1159102
type: ComponentDefinition
persistent identity: BBa K1159102
display id: BBa_K1159102
version: 1
was generated by: iGEM to SBOL conversion
description: C-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: Tabacco Etch Virus
owned by: james
owned by: myers
mutable description: C-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary N-terminal half can be found under BBa_K1159101. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: C-terminal half of TEV Protease
sequence annotation: Ser --> Val for autolysis resistance
sequence: https://synbiohub.org/public/igem/BBa_K1159102_sequence/1

C-terminal half of TEV Protease
type: SequenceAnnotation
persistent identity: annotation2341474
display id: annotation2341474
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341474/range2341474/1
role: SO:0000316
role: feature/protein

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type: Range
persistent identity: range2341474
display id: range2341474
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 1
end: 354
orientation: inline

Ser --> Val for autolysis resistance
type: SequenceAnnotation
persistent identity: annotation2341475
display id: annotation2341475
version: 1
top level: BBa_K1159102
location: https://synbiohub.org/public/igem/BBa_K1159102/annotation2341475/range2341475/1
role: SO:0001059
role: feature/mutation

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type: Range
persistent identity: range2341475
display id: range2341475
version: 1
top level: BBa_K1159102
direction: direction/forward
start: 301
end: 303
orientation: inline

BBa_K1159104
type: ComponentDefinition
persistent identity: BBa K1159104
display id: BBa_K1159104
version: 1
was generated by: iGEM to SBOL conversion
description: N-terminal Phytochrome Interacting Factor 6 (PIF6) in RFC[25]
created: 2013-09-13T11:00:00Z
modified: 2015-05-08T01:09:31Z
mutable provenance: ''Arabidopsis thaliana''
owned by: james
owned by: myers
mutable description: N-terminal Phytochrome Interacting Factor 6 (PIF6) is known to be recognized by Phytochrome B (PhyB) under red light exposition. This interaction between PhyB and PIF6 can be reverted by irradiation with far-red light. This part contains only the first 100 amino acid residues of PIF6 which is sufficient for light-dependent binding to PhyB. N- and C-terminal protein fusions seems not to affect the binding of PhyB. This part is flanked by RFC[25] pre- and suffix for further protein fusions.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PIF6 (2-100 AA)
sequence: https://synbiohub.org/public/igem/BBa_K1159104_sequence/1

PIF6 (2-100 AA)
type: SequenceAnnotation
persistent identity: annotation2343343
display id: annotation2343343
version: 1
top level: BBa_K1159104
location: https://synbiohub.org/public/igem/BBa_K1159104/annotation2343343/range2343343/1
role: feature/protein
role: SO:0000316

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type: Range
persistent identity: range2343343
display id: range2343343
version: 1
top level: BBa_K1159104
direction: direction/forward
start: 1
end: 297
orientation: inline

BBa_K801031
type: ComponentDefinition
persistent identity: BBa K801031
display id: BBa_K801031
version: 1
was generated by: iGEM to SBOL conversion
description: Phytochrome B (PhyB, first 908 N-terminal amino acids)
created: 2012-09-20T11:00:00Z
modified: 2015-05-08T01:13:24Z
mutable provenance: Based on BBa_K365002
owned by: james
owned by: myers
mutable description: improved version of BBa_K365002 to allow N- and C-terminal protein fusions using RFC 25
discontinued: false
dominant: false
experience: experience/None
group u list: _1057_
m user id: 0
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Dong-Jiunn Jeffery TRUONG
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: PhyB (2-908 AA) photosensitive core
sequence annotation: Essential Arg for photoconvertible binding of PIF3/6
sequence annotation: Essential Gly for photoconvertible binding of PIF3/6
sequence annotation: Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
sequence: https://synbiohub.org/public/igem/BBa_K801031_sequence/1

PhyB (2-908 AA) photosensitive core
type: SequenceAnnotation
persistent identity: annotation2343344
display id: annotation2343344
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K801031/annotation2343344/range2343344/1
type: Range
persistent identity: range2343344
display id: range2343344
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1
end: 2721
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198856
display id: annotation2198856
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198856/range2198856/1
type: Range
persistent identity: range2198856
display id: range2198856
version: 1
top level: BBa_K801031
direction: direction/forward
start: 325
end: 327
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198859
display id: annotation2198859
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198859/range2198859/1
type: Range
persistent identity: range2198859
display id: range2198859
version: 1
top level: BBa_K801031
direction: direction/forward
start: 328
end: 330
orientation: inline

Essential Gly for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198861
display id: annotation2198861
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_K801031/annotation2198861/range2198861/1
type: Range
persistent identity: range2198861
display id: range2198861
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1039
end: 1041
orientation: inline

Essential Arg for photoconvertible binding of PIF3/6
type: SequenceAnnotation
persistent identity: annotation2198862
display id: annotation2198862
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198862/range2198862/1
type: Range
persistent identity: range2198862
display id: range2198862
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1051
end: 1053
orientation: inline

Essential Cys for chromophore attachement of phycocyanobilin/phytochromobilin
type: SequenceAnnotation
persistent identity: annotation2198864
display id: annotation2198864
version: 1
top level: BBa_K801031
location: https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K801031/annotation2198864/range2198864/1
type: Range
persistent identity: range2198864
display id: range2198864
version: 1
top level: BBa_K801031
direction: direction/forward
start: 1066
end: 1068
orientation: inline

GSATLinker
type: ComponentDefinition
persistent identity: BBa K243029
display id: BBa_K243029
version: 1
was generated by: iGEM to SBOL conversion
description: GSAT Linker
created: 2009-10-19T11:00:00Z
modified: 2015-05-08T01:11:37Z
mutable provenance: Ordered by Mr.Gene.
owned by: james
owned by: myers
mutable description: This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. The GSAT linker was create to connect our protein domains Fok_a and Fok_i to an function final construct.
discontinued: false
dominant: true
experience: experience/None
group u list: _352_
m user id: 0
owner id: 4732
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Designed for the fusion of proteins or peptides via in frame cloning, according to RFC25.
star: false
creator: Freiburg Bioware09
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence annotation: GSAT Linker
sequence: https://synbiohub.org/public/igem/BBa_K243029_sequence/1

GSAT Linker
type: SequenceAnnotation
persistent identity: annotation2055435
display id: annotation2055435
version: 1
top level: GSATLinker
location: https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K243029/annotation2055435/range2055435/1
type: Range
persistent identity: range2055435
display id: range2055435
version: 1
top level: GSATLinker
direction: direction/forward
start: 1
end: 108
orientation: inline

https://synbiohub.org/public/igem/BBa_K1159120_sequence/1
type: Sequence
persistent identity: BBa K1159120 sequence
display id: BBa_K1159120_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159994_sequence/1
type: Sequence
persistent identity: BBa K1159994 sequence
display id: BBa_K1159994_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagatggccggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159112_sequence/1
type: Sequence
persistent identity: BBa K1159112 sequence
display id: BBa_K1159112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaccggcggagagaatttgtattttcagtcaggaaccggcgttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159116_sequence/1
type: Sequence
persistent identity: BBa K1159116 sequence
display id: BBa_K1159116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159993_sequence/1
type: Sequence
persistent identity: BBa K1159993 sequence
display id: BBa_K1159993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159300_sequence/1
type: Sequence
persistent identity: BBa K1159300 sequence
display id: BBa_K1159300_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaaacagctctggggttgtacccaccccagaggcccacgtggcggctagtactccggtattgcggtacccttgtacgcctgttttatactcccttcccgtaacttagacgcacaaaaccaagttcaatagaagggggtacaaaccagtaccaccacgaacaagcacttctgtttccccggtgatgtcgtatagactgcttgcgtggttgaaagcgacggatccgttatccgcttatgtacttcgagaagcccagtaccacctcggaatcttcgatgcgttgcgctcagcactcaaccccagagtgtagcttaggctgatgagtctggacatccctcaccggtgacggtggtccaggctgcgttggcggcctacctatggctaacgccatgggacgctagttgtgaacaaggtgtgaagagcctattgagctacataagaatcctccggcccctgaatgcggctaatcccaacctcggagcaggtggtcacaaaccagtgattggcctgtcgtaacgcgcaagtccgtggcggaaccgactactttgggtgtccgtgtttccttttattttattgtggctgcttatggtgacaatcacagattgttatcataaagcgaattggatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159301_sequence/1
type: Sequence
persistent identity: BBa K1159301 sequence
display id: BBa_K1159301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159302_sequence/1
type: Sequence
persistent identity: BBa K1159302 sequence
display id: BBa_K1159302_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159996_sequence/1
type: Sequence
persistent identity: BBa K1159996 sequence
display id: BBa_K1159996_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accggttaatactagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159318_sequence/1
type: Sequence
persistent identity: BBa K1159318 sequence
display id: BBa_K1159318_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagagaatttgtattttcagtcagga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159114_sequence/1
type: Sequence
persistent identity: BBa K1159114 sequence
display id: BBa_K1159114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159106_sequence/1
type: Sequence
persistent identity: BBa K1159106 sequence
display id: BBa_K1159106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159108_sequence/1
type: Sequence
persistent identity: BBa K1159108 sequence
display id: BBa_K1159108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaactaccggcggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggcaccggcatgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159101_sequence/1
type: Sequence
persistent identity: BBa K1159101 sequence
display id: BBa_K1159101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggagaaagcttgtttaaggggccgcgtgattacaacccgatatcgagcaccatttgtcatttgacgaatgaatctgatgggcacacaacatcgttgtatggtattggatttggtcccttcatcattacaaacaagcacttgtttagaagaaataatggaacactgttggtccaatcactacatggtgtattcaaggtcaagaacaccacgactttgcaacaacacctcattgatgggagggacatgataattattcgcatgcctaaggatttcccaccatttcctcaaaagctgaaatttagagagccacaaagggaagagcgcatatgtcttgtgacaaccaacttccaaact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159102_sequence/1
type: Sequence
persistent identity: BBa K1159102 sequence
display id: BBa_K1159102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagagcatgtctagcatggtgtcagacactagctgcacattcccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccattagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaagcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttggcgattaaatgctgactcagtattgtgggggggccataaagttttcatggtgaaacctgaagagccttttcagccagttaaggaagcgactcaactcatgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159104_sequence/1
type: Sequence
persistent identity: BBa K1159104 sequence
display id: BBa_K1159104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttcttaccaaccgattattgttgcaggttaagcgatcaagagtatatggagcttgtgtttgagaatggccagattcttgcaaagggccaaagatccaacgtttctctgcataatcaacgtaccaaatcgatcatggatttgtatgaggcagagtataacgaggatttcatgaagagtatcatccatggtggtggtggtgccatcacaaatctcggggacacgcaggttgttccacaaagtcatgttgctgctgcccatgaaacaaacatgttggaaagcaataaacatgttgac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K801031_sequence/1
type: Sequence
persistent identity: BBa K801031 sequence
display id: BBa_K801031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtttccggagtcgggggtagtggcggtggccgtggcggtggccgtggcggagaagaagaaccgtcgtcaagtcacactcctaataaccgaagaggaggagaacaagctcaatcgtcgggaacgaaatctctcagaccaagaagcaacactgaatcaatgagcaaagcaattcaacagtacaccgtcgacgcaagactccacgccgttttcgaacaatccggcgaatcagggaaatcattcgactactcacaatcactcaaaacgacgacgtacggttcctctgtacctgagcaacagatcacagcttatctctctcgaatccagcgaggtggttacattcagcctttcggatgtatgatcgccgtcgatgaatccagtttccggatcatcggttacagtgaaaacgccagagaaatgttagggattatgcctcaatctgttcctactcttgagaaacctgagattctagctatgggaactgatgtgagatctttgttcacttcttcgagctcgattctactcgagcgtgctttcgttgctcgagagattaccttgttaaatccggtttggatccattccaagaatactggtaaaccgttttacgccattcttcataggattgatgttggtgttgttattgatttagagccagctagaactgaagatcctgcgctttctattgctggtgctgttcaatcgcagaaactcgcggttcgtgcgatttctcagttacaggctcttcctggtggagatattaagcttttgtgtgacactgtcgtggaaagtgtgagggacttgactggttatgatcgtgttatggtttataagtttcatgaagatgagcatggagaagttgtagctgagagtaaacgagacgatttagagccttatattggactgcattatcctgctactgatattcctcaagcgtcaaggttcttgtttaagcagaaccgtgtccgaatgatagtagattgcaatgccacacctgttcttgtggtccaggacgataggctaactcagtctatgtgcttggttggttctactcttagggctcctcatggttgtcactctcagtatatggctaacatgggatctattgcgtctttagcaatggcggttataatcaatggaaatgaagatgatgggagcaatgtagctagtggaagaagctcgatgaggctttggggtttggttgtttgccatcacacttcttctcgctgcataccgtttccgctaaggtatgcttgtgagtttttgatgcaggctttcggtttacagttaaacatggaattgcagttagctttgcaaatgtcagagaaacgcgttttgagaacgcagacactgttatgtgatatgcttctgcgtgactcgcctgctggaattgttacacagagtcccagtatcatggacttagtgaaatgtgacggtgcagcatttctttaccacgggaagtattacccgttgggtgttgctcctagtgaagttcagataaaagatgttgtggagtggttgcttgcgaatcatgcggattcaaccggattaagcactgatagtttaggcgatgcggggtatcccggtgcagctgcgttaggggatgctgtgtgcggtatggcagttgcatatatcacaaaaagagactttcttttttggtttcgatctcacactgcgaaagaaatcaaatggggaggcgctaagcatcatccggaggataaagatgatgggcaacgaatgcatcctcgttcgtcctttcaggcttttcttgaagttgttaagagccggagtcagccatgggaaactgcggaaatggatgcgattcactcgctccagcttattctgagagactcttttaaagaatctgaggcggctatgaactctaaagttgtggatggtgtggttcagccatgtagggatatggcgggggaacaggggattgatgagttaggtgcagttgcaagagagatggttaggctcattgagactgcaactgttcctatatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K243029_sequence/1
type: Sequence
persistent identity: BBa K243029 sequence
display id: BBa_K243029_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtggttctgccggtggctccggttctggctccagcggtggcagctctggtgcgtccggcacgggtactgcgggtggcactggcagcggttccggtactggctctggc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z