LOCUS       BBa_K1159202             412 bp    DNA     linear       25-Nov-2024
DEFINITION  Secretory SpyCatcher (IgKappa-SigP_SpyCatcher) in RFC[25] N-Part
ACCESSION   BBa_K1159202
VERSION     BBa_K1159202.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     CDS             74..412
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    173..175
                     /label=Iso --> Glu
     misc_feature    278..280
                     /label=Met --> Tyr
     misc_feature    164..166
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             74..412
                     /label=BBa_K1159200
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgttgata ccttatcagg tttatcaagt gagcaaggtc agtccggtga
      121 tatgacaatt gaagaagata gtgctaccca tattaaattc tcaaaacgtg atgaggacgg
      181 caaagagtta gctggtgcaa ctatggagtt gcgtgattca tctggtaaaa ctattagtac
      241 atggatttca gatggacaag tgaaagattt ctacctgtat ccaggaaaat atacatttgt
      301 cgaaaccgca gcaccagacg gttatgaggt agcaactgct attaccttta cagttaatga
      361 gcaaggtcag gttactgtaa atggcaaagc aactaaaggt gacgctcata tt
//