LOCUS BBa_K1159204 445 bp DNA linear 22-Nov-2024 DEFINITION Secretory SpyCatcher (SERK-SigP_SpyCatcher) in RFC[25] N-Part ACCESSION BBa_K1159204 VERSION BBa_K1159204.1 FEATURES Location/Qualifiers RBS 1..4 /label=Consensus Sequence for ''P. patens'' CDS 5..100 /label=SERK Signal Peptide misc_feature 1..100 /label=BBa_K1159303 misc_feature 101..106 /label=25/25 scar misc_feature 101..106 /label=BBa_K1159993 CDS 107..445 /label=N-terminal FbaB (modified SpyCatcher) misc_feature 206..208 /label=Iso --> Glu misc_feature 311..313 /label=Met --> Tyr misc_feature 197..199 /label=Reactive Lys for isopeptide forming reaction CDS 107..445 /label=BBa_K1159200 ORIGIN 1 aacaatgcct ggtgaagttg cttggtggag gcctcttttc cttatcgctc ttatgcctat 61 cggagtgctc tctaacgctg agggtgatgc tcttaacacc accggcgttg ataccttatc 121 aggtttatca agtgagcaag gtcagtccgg tgatatgaca attgaagaag atagtgctac 181 ccatattaaa ttctcaaaac gtgatgagga cggcaaagag ttagctggtg caactatgga 241 gttgcgtgat tcatctggta aaactattag tacatggatt tcagatggac aagtgaaaga 301 tttctacctg tatccaggaa aatatacatt tgtcgaaacc gcagcaccag acggttatga 361 ggtagcaact gctattacct ttacagttaa tgagcaaggt caggttactg taaatggcaa 421 agcaactaaa ggtgacgctc atatt //