BBa_K1159205 1 BBa_K1159205 Secretory SpyTag (SERK-SigP_SpyTag) in RFC[25] N-Part 2013-09-16T11:00:00Z 2015-06-11T01:59:37Z Synthetic This part encodes the SpyTag [http://parts.igem.org/Part:BBa_K1159201 BBa_K1159201] with a N-terminal signal peptide for secretion (SERK receptor signal peptide from ''Physcomitrella patens''). This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 4206 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2348900 1 BBa_K1159993 component2348898 1 BBa_K1159303 component2348903 1 BBa_K1159201 annotation2348900 1 BBa_K1159993 range2348900 1 101 106 annotation2348903 1 BBa_K1159201 range2348903 1 107 145 annotation2348898 1 BBa_K1159303 range2348898 1 1 100 BBa_K1159311 1 BBa_K1159311 GFPmut1 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z ''Aequarea victoria'' This part codes for GFPmut1 which is flanked by RFC[25] pre- and suffix. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2343590 1 GFPmut1 range2343590 1 1 711 BBa_K1159309 1 BBa_K1159309 19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25] 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic This part encodes a 19 amino acids long linker with a N-terminal ''Strep''-tag II and a C-terminal cleavage site for the TEV Protease. For further protein fusions this part is flanked by RFC[25} pre- and suffix. false false _1471_ 0 11507 9 In stock false x false TU Munich 2013 annotation2342591 1 TEV cleavage site range2342591 1 34 54 annotation2342578 1 ''Strep''-tag II range2342578 1 7 30 BBa_K1159207 1 BBa_K1159207 Membrane-anchored SpyTag in RFC[25] N-Part 2013-09-16T11:00:00Z 2015-06-11T01:58:39Z Synthetic This part codes for a membrane-anchored version of SpyTag [http://parts.igem.org/wiki/index.php?title=Part:BBa_K1159201 BBa_K1159201] for the chassis ''Physcomitrella patens''. Please see subparts for for further description. This construct is flanked by RFC[25] N-part prefix and suffix. Note: This means only protein fusions to the C-terminus of this part is possible, adding promoters (typically RFC[10]) into the prefix or terminators/IRES (typically RFC[10]) into the suffix of this part is nevertheless possible. false false _1471_ 4206 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG component2349003 1 BBa_K1159316 component2348984 1 BBa_K1159205 component2348986 1 BBa_K1159993 annotation2348984 1 BBa_K1159205 range2348984 1 1 145 annotation2349003 1 BBa_K1159316 range2349003 1 152 1147 annotation2348986 1 BBa_K1159993 range2348986 1 146 151 BBa_K1159993 1 BBa_K1159993 25/25 Assembly Scar 2013-09-14T11:00:00Z 2015-05-08T01:09:31Z Synthetic Assembly scar between RFC[25] Biobrick and RFC[25] Biobrick. false false _1471_ 0 11507 9 Not in stock false x false TU Munich 2013 annotation2342863 1 25/25 scar range2342863 1 1 6 BBa_K1159201 1 BBa_K1159201 Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343481 1 SpyTag range2343481 1 1 39 annotation2343512 1 Reactive Asp for isopeptide forming reaction range2343512 1 19 21 BBa_K1159303 1 BBa_K1159303 Signal Peptide of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:49Z ''Physcomitrella patens'' This part codes for the signal peptide from the SERK receptor (Somatic Embryogenesis RECEPTOR KINAS) of ''Physocmitrella patens''. The signal peptide induces translocation of the protein into the Endoplasmatic Reticulum (ER) for secretion or membrane integration. Protein fused to the C-terminus of this part should be secreted or otherwise if the part fused region contains a transmembrane domain it will be integrated in cytoplasmatic membrane. This part starts with a consensus sequence for ''Physcomitrella patens'' which allows efficient translation start. Furthermore, this part is flanked by RFC[25] N-part pre- and suffix for fusions to the C-terminus of this part. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2341512 1 Consensus Sequence for ''P. patens'' range2341512 1 1 4 annotation2341513 1 SERK Signal Peptide range2341513 1 5 100 BBa_K1159305 1 BBa_K1159305 Transmembrane domain of SERK Receptor from <i>Physcomitrella patens</i> in RFC[25] 2013-09-13T11:00:00Z 2015-06-11T01:54:11Z ''Physcomitrella patens'' This part is encoding the transmembrane region of the SERK Receptor from ''Physcomitrella patens''. For membrane translocation a N-terminal signal peptide is still necessary for translocation through the ER membrane. This part is flanked by RFC[25] pre- and suffix. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2343588 1 Transmembrane Domain of SERK Receptor from ''P. patens'' range2343588 1 1 186 BBa_K1159316 1 BBa_K1159316 SERK-Receptor TMD with C-terminal GFP and N-terminal <i>Strep</i>-tag II TEV-site linker in RFC[25] 2013-09-15T11:00:00Z 2015-06-11T01:53:10Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from ''Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion and a N-terminal fusion to a linker containing ''Strep''-tag II and a TEV cleavage site; SERK TMD and GFPmut1 are seperated by a middle long linker. This part is flanked by RFC[25] pre- and suffix for further protein fusions. Note: A signal peptide is needed for membrane integration. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346350 1 BBa_K1159309 component2346352 1 BBa_K1159993 component2346363 1 BBa_K1159315 annotation2346352 1 BBa_K1159993 range2346352 1 58 63 annotation2346363 1 BBa_K1159315 range2346363 1 64 996 annotation2346350 1 BBa_K1159309 range2346350 1 1 57 BBa_K243005 1 MiddleLink Middle Linker ( Gly-Gly-Ser-Gly)x2 2009-10-13T11:00:00Z 2015-05-08T01:11:37Z Oligos synthesized by sigma. Hybridized by PCR. This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly . false false _352_ 0 4732 9 It's complicated false None. false Freiburg Bioware09 annotation2041884 1 MiddleLinker range2041884 1 1 24 BBa_K1159315 1 BBa_K1159315 TMD of SERK Receptor from <i>Physcomitrella patens</i> with C-terminal GFP fusion in RFC[25] 2013-09-15T11:00:00Z 2015-05-28T03:06:34Z Synthetic This part codes for the Transmembrane Domain of SERK Receptor from <i>Physcomitrella patens'' with a C-terminal (intracellular) GFPmut1 fusion; SERK TMD and GFPmut1 are seperated by a middle long linker. Still, a signal peptide is needed for membrane integration. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 component2346338 1 BBa_K243005 component2346334 1 BBa_K1159305 component2346340 1 BBa_K1159993 component2346336 1 BBa_K1159993 component2346342 1 BBa_K1159311 annotation2346342 1 BBa_K1159311 range2346342 1 223 933 annotation2346340 1 BBa_K1159993 range2346340 1 217 222 annotation2346338 1 BBa_K243005 range2346338 1 193 216 annotation2346336 1 BBa_K1159993 range2346336 1 187 192 annotation2346334 1 BBa_K1159305 range2346334 1 1 186 BBa_K1159207_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaagaccggcagcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159993_sequence 1 accggc BBa_K1159315_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159303_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacacc BBa_K243005_sequence 1 ggtggttctggtggtggttctggt BBa_K1159309_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcgga BBa_K1159205_sequence 1 aacaatgcctggtgaagttgcttggtggaggcctcttttccttatcgctcttatgcctatcggagtgctctctaacgctgagggtgatgctcttaacaccaccggcgctcatattgtcatggttgatgcttacaagccaactaag BBa_K1159311_sequence 1 agtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159305_sequence 1 cctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggct BBa_K1159316_sequence 1 agcgcttggtctcatcctcaatttgaaaagggtgaaaatctttattttcaaagcggaaccggccctggacaacctccttttcctcctcctcctccttttacaccacctcctccacaaactcctaacggtgcttctggtgagaactctactggtgctattgctggtggtgttgctgctggtgcagctcttttgtttgctgctcctgctattggattcgcttggtggagaagaagaaggcctattgaggctaccggcggtggttctggtggtggttctggtaccggcagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttcaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgagctctacaaa BBa_K1159201_sequence 1 gctcatattgtcatggttgatgcttacaagccaactaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z