LOCUS       BBa_K1159208             628 bp    DNA     linear       22-Dec-2024
DEFINITION  Secretory SpyTag_nLuc fusion (IgKappa-SigP_SpyTag_nLuc) in RFC[25] 
            N-Part
ACCESSION   BBa_K1159208
VERSION     BBa_K1159208.1  
FEATURES             Location/Qualifiers
     RBS             1..4
                     /label=Consensus Sequence for P. patens
     CDS             5..67
                     /label=Ig Kappa Signal Peptide
     misc_feature    1..67
                     /label=BBa_K1159304
     misc_feature    68..73
                     /label=25/25 scar
     misc_feature    68..73
                     /label=BBa_K1159993
     misc_feature    74..112
                     /label=SpyTag
     misc_feature    92..94
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             74..112
                     /label=BBa_K1159201
     CDS             1..112
                     /label=BBa_K1159203
     misc_feature    113..118
                     /label=25/25 scar
     misc_feature    113..118
                     /label=BBa_K1159993
     CDS             119..628
                     /label=NanoLuc Luciferase
     CDS             119..628
                     /label=BBa_K1159001
ORIGIN
        1 aacaatggaa accgatactc tcctcctctg ggttctcttg ctttgggttc caggatctac
       61 cggcgatacc ggcgctcata ttgtcatggt tgatgcttac aagccaacta agaccggcgt
      121 gttcaccctc gaggatttcg ttggagattg gagacagacc gctggataca accttgatca
      181 ggttttggag cagggtggtg tgtctagtct tttccagaac ctcggagtgt ctgtgacccc
      241 tatccagaga atcgttctct ctggtgagaa cggactcaag atcgatatcc acgtgatcat
      301 cccttacgag ggactctcag gtgatcagat gggacagatc gagaagattt tcaaggtggt
      361 gtaccctgtt gatgatcacc acttcaaggt gatcctccac tacggaactc tcgtgatcga
      421 tggtgtgacc ccaaacatga tcgattactt cggaaggcca tacgagggaa tcgctgtgtt
      481 cgatggaaag aagattaccg ttaccggaac cctctggaac ggaaacaaga ttatcgatga
      541 gaggctcatc aaccctgatg gatctctctt gttcagggtg accatcaacg gtgtgactgg
      601 ttggagactt tgcgagagaa tccttgct
//